Dynamic gene expression in fish muscle during recovery growth induced by a fasting-refeeding schedule

Rescan, Pierre‐Yves; Montfort, Jérôme; Rallière, Cécile; Cam, Aurélie Le; Esquerré, Diane; Hugot, Karine

doi:10.1186/1471-2164-8-438

Cited by 97 publications

(96 citation statements)

References 38 publications

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“…Hybridization of microarrays and raw data production " cDNA nylon membrane microarrays were generated by CRB GADIE (http://crb-gadie.inra.fr/) as described previously (Rescan et al 2007). Prehybridization of the membranes was performed at 65 8C for 4 h in 5!…”

Section: Steroid Measurementmentioning

confidence: 99%

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Sambroni¹,

Rolland²,

Lareyre³

et al. 2012

Journal of Molecular Endocrinology

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The general rules established from mammalian species for the regulation of spermatogenesis by gonadotropins may not be fully relevant in fish. Particularly, Fsh is as potent as Lh to stimulate steroidogenesis and the Fsh receptor is expressed in Leydig cells. In seasonal breeders, Fsh is likely the major gonadotropin involved in spermatogenesis onset and Lh is required to support spermatogenesis progression and gamete release. However, the genes that relay the action of Fsh and Lh have been poorly investigated in fish. The present study was aimed at identifying gonadotropin-dependent genes expressed in the testis during fish puberty. We cultured pubertal trout testicular explants for 96 h, with or without gonadotropin, and analyzed transcriptome variations using microarrays. Fsh and Lh had similar effects on a large group of genes while other genes were preferentially regulated by one or the other gonadotropin. We showed that most of the responsive genes were expressed in somatic cells and exhibited relevant patterns during the seasonal reproductive cycle. Some genes preferentially modulated by Lh could be involved in testicular cell fate (pvrl1 and bty) or sperm maturation (ehmt2 and racgap1) and will deserve further examination. Besides Fsh's effects on the steroidogenic pathway, our study demonstrates that Fsh coordinates relevant stimulatory and inhibitory paracrine factors known to regulate early germ cell proliferation and differentiation. Some of these genes belong to major regulatory pathways including the Igf pathway (igf1b/igf3 and igfbp6), the Tgfb pathway (amh, inha, inhba, and fstl3), the Wnt pathway (wisp1), and pleiotrophin (mdka).

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Section: Steroid Measurementmentioning

confidence: 99%

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Sambroni¹,

Rolland²,

Lareyre³

et al. 2012

Journal of Molecular Endocrinology

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“…Fasting induces a loss of muscle mass resulting from the breakdown of muscle proteins, which involves several proteolytic systems such as the autophagy-lysosome and the ubiquitin-proteasome systems (Bonaldo and Sandri, 2013;Bodine and Baehr, 2014). Some studies have addressed changes in transcriptional regulation associated with muscle atrophy (Salem Rescan et al, 2007), but less is known about concomitant changes in muscle fibre production. In this study, we show that GFP-expressing small myofibres are still produced in the muscle of fasted trout and are as numerous as they are in the muscle of well-fed trout.…”

Section: Discussionmentioning

confidence: 99%

Analysis of muscle fibre input dynamics using amyog: GFP transgenic trout model

Rescan

Rallière

Lebret

et al. 2015

Journal of Experimental Biology

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The dramatic increase in myotomal muscle mass in teleosts appears to be related to their sustained ability to produce new fibres in the growing myotomal muscle. To describe muscle fibre input dynamics in trout (Oncorhynchus mykiss), we generated a stable transgenic line carrying green fluorescent protein (GFP) cDNA driven by the myogenin promoter. In this myog:GFP transgenic line, muscle cell recruitment is revealed by the appearance of fluorescent, small, nascent muscle fibres. The myog:GFP transgenic line displayed fibre formation patterns in the developing trout and showed that the production of new fluorescent myofibres (muscle hyperplasia) is prevalent in the juvenile stage but progressively decreases to eventually cease at approximately 18 months post-fertilisation. However, fluorescent, nascent myofibres were formed de novo in injured muscle of aged trout, indicating that the inhibition of myofibre formation associated with trout ageing cannot be attributed to the lack of recruitable myogenic cells but rather to changes in the myogenic cell microenvironment. Additionally, the myog:GFP transgenic line demonstrated that myofibre production persists during starvation.

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“…Recovery growth in muscle in response to fasting and re-feeding was studied in trout using a cDNA microarray and revealed a complex response. The principal groups of upregulated transcripts were involved in protein catabolism, myofibre and muscle remodelling and regulation of transcription (Rescan et al, 2007). Other studies of re-feeding in fish have shown enhanced muscle expression of kinases involved in the mTOR signalling pathway known to promote protein accretion in mammalian muscle (Seiliez et al, 2008).…”

Section: Consequences Of Maternal Nutritionmentioning

confidence: 94%

Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. I. Regulation of myogenesis and environmental impact

Rehfeldt

Pas

Wimmers

et al. 2011

Animal

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Skeletal muscle development in vertebrates – also termed myogenesis – is a highly integrated process. Evidence to date indicates that the processes are very similar across mammals, poultry and fish, although the timings of the various steps differ considerably. Myogenesis is regulated by the myogenic regulatory factors and consists of two to three distinct phases when different fibre populations appear. The critical times when myogenesis is prone to hormonal or environmental influences depend largely on the developmental stage. One of the main mechanisms for both genetic and environmental effects on muscle fibre development is via the direct action of the growth hormone–insulin-like growth factor (GH–IGF) axis. In mammals and poultry, postnatal growth and function of muscles relate mainly to the hypertrophy of the fibres formed during myogenesis and to their fibre-type composition in terms of metabolic and contractile properties, whereas in fish hyperplasia still plays a major role. Candidate genes that are important in skeletal muscle development, for instance, encode for IGFs and IGF-binding proteins, myosin heavy chain isoforms, troponin T, myosin light chain and others have been identified. In mammals, nutritional supply in utero affects myogenesis and the GH–IGF axis may have an indirect action through the partitioning of nutrients towards the gravid uterus. Impaired myogenesis resulting in low skeletal myofibre numbers is considered one of the main reasons for negative long-term consequences of intrauterine growth retardation. Severe undernutrition in utero due to natural variation in litter or twin-bearing species or insufficient maternal nutrient supply may impair myogenesis and adversely affect carcass quality later in terms of reduced lean and increased fat deposition in the progeny. On the other hand, increases in maternal feed intake above standard requirement seem to have no beneficial effects on the growth of the progeny with myogenesis not or only slightly affected. Initial studies on low and high maternal protein feeding are published. Although there are only a few studies, first results also reveal an influence of nutrition on skeletal muscle development in fish and poultry. Finally, environmental temperature has been identified as a critical factor for growth and development of skeletal muscle in both fish and poultry.

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Dynamic gene expression in fish muscle during recovery growth induced by a fasting-refeeding schedule

Cited by 97 publications

References 38 publications

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Analysis of muscle fibre input dynamics using amyog: GFP transgenic trout model

Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. I. Regulation of myogenesis and environmental impact

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