Dynamic Interface Imprinting: High‐Affinity Peptide Binding Sites Assembled by Analyte‐Induced Recruiting of Membrane Receptors

Abstract

Come together: Dynamic molecular recognition events at biological membrane receptors play a key role in cell signaling. Artificial membranes have been prepared with embedded synthetic receptors which dynamically arrange and selectively respond to external stimuli, such as, small peptide ligands.

New Amphiphilic Bowl-Shaped Receptors on the Basis of Calix[4]arenes in Cone Conformation: Synthesis, Self-Aggregation and Eosin Y Dye Binding

“…1 H NMR spectra is in agreement with literature. [15] 5,17-Di-tert-butyl -11,23-diamino-25,27-dibutyloxy-26,28-dihydroxycalix [4]arene (3). THF solution of compound 2 (0.05 M) was pumped through the flow hydrogenation reactor at a temperature of 120 °C and a hydrogen pressure 30 bar at 1 ml/min flow rate.…”

“…Moreover, such amphiphilic synthetic receptors can be used as a parts of multifunctional phospholipid vesicles, which attract a considerable interest due to their use in molecular recognition, drug delivery, cell mimics, catalysis and many other applications. [1][2][3] Selective functionalization of both upper and lower rim in the cone conformation with maintaining the cavity for better host-guest interactions makes calixarenes one of the best platforms for the amphiphilic receptors design. The unique structure and a variety of stereoisomeric forms of calix [4]arene derivatives led to their wide applications in supramolecular chemistry.…”

Supramolecular Chemistry in the Biomembrane

“…König and co‐workers also tested whether bis‐Zn II cyclen complex 7 , which could bind to phosphoserine and histidine residues in peptides, would provide several binding sites for peptides with both residues, and thus, enhance affinity to vesicle‐bound receptors . If the receptors were embedded into DSPC lipids, a relatively high receptor concentration of 10 mol % was required to observe an increased binding affinity, whereas, in DOPC lipids, a significant enhancement was already observed for only 1 mol % of receptors.…”

Molecularly Imprinted Materials for Selective Biological Recognition

“…The main design obstacles of synthetic materials with microbial identification characteristics are the large target, fragile self‐assembly system and limited material properties . Surface and interface imprinting methods for proteins mentioned above have solved the difficulties of biomacromolecular imprinting . However, it is undeniable that imprinting of larger biological entities with huge sizes is still very challenging.…”