The eastern Pamir Plateau, with a mean altitude of 5000 m, forms a large-scale glacier region with a size of 2054 km 2 that serves as an important environmental climate condition for central Asia. In this study, we estimated the spatio-temporal patterns of glacier activities in this region by using the time series sub-pixel offsets derived from 63 Sentinel-2 optical images between December 31, 2020 and December 31, 2021. Our results indicated the mean glacier flow velocity in this region was 0.531 m/d ± 0.007 m/d in 2021, and the Kongur Tagh Glacier was much more active than the Kingata Glacier and Muztagh Ata Glacier. The time series observations revealed that the glacier motion involves a pre-melting period (January-April) with a velocity of 0.600 m/d ± 0.012 m/d, a melting period (May-August) with a velocity of 0.608 m/d ± 0.003 m/d, and a post-melting period (September-December) with a velocity of 0.659 m/d ± 0.006 m/d. In order to get insights into the characteristics of these glacier activities, we carried out a correlation analysis between the glacier flow velocity change and its potential caused reasons (i.e., topography, temperature, precipitation, glacier surges, debriscover, and glacier thickness), and current results suggest that the glacier flow velocity is influenced by a combination of these factors.