“…MAC-ELISA Anti-dengue IgG detection is also conducted by fixed IgG via anti-human IgG antibody that was immobilized earlier on the plate wells. However, dengue-specific IgG tests are less specific than IgM because IgG is cross-reactive and has no conserved epitopes among the four serotypes, unlike IgM, which interacts only with epitopes of the infecting serotype present [45]. The performance and the specificity of classical serological techniques for dengue diagnosis depend on many factors, such as the quality of the antigens that are utilized with the ELISA method, the specimen type (e.g., serum, whole blood or saliva), the DENV serotype [10] and the IgM and IgG titers [28].…”