Dynamic Light Scattering and X-ray Photoelectron Spectroscopy Characterization of PEGylated Polymer Nanocarriers: Internal Structure and Surface Properties

Celasco, Edvige; Valente, Ilaria; Marchisio, Daniele; Barresi, Antonello

doi:10.1021/la501198v

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…This quantitative analysis was carried out by monitoring the peaks in the N1s region of the spectrum (400 eV), and normalizing the peaks with the sensitivity factor of the XPS analyzer. The methodology through which the peaks were integrated and the geometry was optimized has been extensively described in our previous work [55]. The surface composition estimated by this method was then compared with the overall loading capacity to understand whether caffeine was more concentrated in the outer layer or evenly distributed in the core.…”

Section: Resultsmentioning

confidence: 99%

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

et al. 2018

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Flash nanoprecipitation (FNP) is a widely used technique to prepare particulate carriers based on various polymers, and it was proven to be a promising technology for the industrial production of drug loaded nanoparticles. However, up to now, only its application to hydrophobic compounds has been deeply studied and the encapsulation of some strongly hydrophilic compounds, such as caffeine, remains a challenge. Caffeine loaded poly-ε-caprolactone (PCL) nanoparticles were produced in a confined impinging jet mixer using acetone as the solvent and water as the antisolvent. Caffeine was dissolved either in acetone or in water to assess the effects of two different process conditions. Nanoparticles properties were assessed in terms of loading capacity (LC%), encapsulation efficiency (EE%), and in vitro release kinetics. Samples were further characterized by dynamic light scattering, scanning electron microscopy, X-ray photo electron spectroscopy, and infrared spectroscopy to determine the size, morphology, and structure of nanoparticles. FNP was proved an effective technique for entrapping caffeine in PCL and to control its release behavior. The solvent used to solubilize caffeine influences the final structure of the obtained particles. It was observed that the active principle was preferentially adsorbed at the surface when using acetone, while with water, it was embedded in the matrix structure. The present research highlights the possibility of extending the range of applications of FNP to hydrophilic molecules.

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Section: Resultsmentioning

confidence: 99%

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

et al. 2018

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“…XPS has been extensively applied to study surface chemistry in a wide variety of fields, such as electronics, forensics, and biology, to name a few. Nevertheless, since conventional XPS must maintain a UHV environment, the method is limited to solid samples and focuses on examining surfaces of semiconductors, thin films and coating (i.e., metal/metal oxide/DNA film), − biosensors, and dry powder pharmaceutical materials. − …”

Section: Introductionmentioning

confidence: 99%

Surface Analysis of Nanocomplexes by X-ray Photoelectron Spectroscopy (XPS)

Korin

Froumin

Cohen

2017

ACS Biomater. Sci. Eng.

141

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Self-assembled nanocomplexes composed of individual molecules that spontaneously connect via noncovalent interactions have recently emerged as versatile alternatives to conventional controlled drug delivery systems because of their unique bioinspired properties (responsiveness, dynamics, etc.). Characterization of such nanocomplexes typically includes their size distribution, surface charge, morphology, drug entrapment efficiency, and verification of the coexistence of labeled components within the nanocomplexes using a colocalization study. Less common is the direct examination of the molecular interactions between the different components in the coassembled nanocomplex, especially in nanocomplexes composed of hygroscopic components, because convenient methods are still lacking. Here, we present a detailed experimental protocol for determining the surface composition and the chemical bonds by X-ray photoelectron spectroscopy (XPS) after drying the deposit hygroscopic sample overnight under UHV. We applied this method to investigate the surface chemistry of binary Ca 2+ -siRNA nanocomplexes and ternary nanocomplexes of hyaluronan-sulfate (HAS)-Ca 2+ -siRNA, deposited on a wafer. Notably, we showed that the protocol can be implemented to study the surface composition and interactions of the deposited nanocomplexes with a traditional XPS instrument, and it requires only a relatively small amount of the nanocomplex suspension.

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“…Each element demonstrates characteristic peaks and relative abundance when exposed to xrays (Andrade, 1985). Celasco et al (2014) used XPS for measuring surface texture and copolymer thickness in polyethylene glycol (PEG) nanocapsules prepared by solvent displacement method (Celasco et al, 2014). Miao et al (2020) analyzed the elemental composition of PVC MPs before and after electrocatalytic oxidation using high-resolution XPS (Miao et al, 2020).…”

Section: Elemental Compositionmentioning

confidence: 99%

Recent Advances in Photocatalytic Removal of Microplastics: Mechanisms, Kinetic Degradation, and Reactor Design

Hamd

Daher

Tofa³

et al. 2022

Front. Mar. Sci.

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Plastic products are used in almost all aspects of our daily life. Due to their low cost, portability, durability, and resistance to degradation, these products are affecting the health of the environment and biota on a global scale. Thus, the removal and mineralization of microplastics is an important challenge in the 21st century. Advanced oxidation processes (AOPs) have recently been identified as a viable treatment technique for tackling recalcitrant organic molecules and polymers. However, information on kinetic degradation mechanisms and photocatalytic reactor design is insufficient. This review discusses the fundamentals of photocatalysis and photo-Fenton processes in addition to the photocatalytic degradation mechanisms. We also introduce different characterization techniques of the major microplastic pollutants such as PE, PP, PVC, PS, PMMA, and PA66. In addition, a detailed overview of the major existing photocatalytic plants and the scaling-up methods of photoreactors are discussed.

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Dynamic Light Scattering and X-ray Photoelectron Spectroscopy Characterization of PEGylated Polymer Nanocarriers: Internal Structure and Surface Properties

Cited by 9 publications

References 28 publications

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

Surface Analysis of Nanocomplexes by X-ray Photoelectron Spectroscopy (XPS)

Recent Advances in Photocatalytic Removal of Microplastics: Mechanisms, Kinetic Degradation, and Reactor Design

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