Dynamic live-cell super-resolution imaging with parallelized fluorescence emission difference microscopy

He, Minfei; Han, Yubing; Gan, Yanhong; Zhang, Zhimin; Liu, Wenjie; Xu, Liang; Kuang, Cuifang; Hao, Xiang; Liu, Xu

doi:10.1016/j.optcom.2019.125087

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…[ 11 , 13 ] For instance, structured illumination microscopy (SIM) revealed new, direct, and functional contact forms between the mitochondria and lysosomes, which can increase the local viscosity of mitochondria. [ 14 ] SIM in living cells can also be used to quantify subcellular dynamics, [ 15 ] not to mention support drug screening. [ 16 , 17 ] Since SIM improves the resolution by using optics and analysis and does not require any nonlinear response of fluorophore to the light intensity, [ 18 ] SIM has gained popularity in cell biology.…”

Section: Introductionmentioning

confidence: 99%

A Continuous Add‐On Probe Reveals the Nonlinear Enlargement of Mitochondria in Light‐Activated Oncosis

et al. 2021

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Oncosis, depending on DNA damage and mitochondrial swelling, is an important approach for treating cancer and other diseases. However, little is known about the behavior of mitochondria during oncosis, due to the lack of probes for in situ visual illumination of the mitochondrial membrane and mtDNA. Herein, a mitochondrial lipid and mtDNA dual-labeled probe, MitoMN, and a continuous add-on assay, are designed to image the dynamic process of mitochondria in conditions that are unobservable with current mitochondrial probes. Meanwhile, the MitoMN can induce oncosis in a light-activated manner, which results in the enlargement of mitochondria and the death of cancer cells. Using structured illumination microscopy (SIM), MitoMN-stained mitochondria with a dual-color response reveals, for the first time, how swelled mitochondria interacts and fuses with each other for a nonlinear enlargement to accelerate oncosis into an irreversible stage. With this sign of irreversible oncosis revealed by MitoMN, oncosis can be segregated into three stages, including before oncosis, initial oncosis, and accelerated oncosis.

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