DYNAMIC MATHEMATICAL MODELLING OF REACTION KINETICS FOR CYCLODEXTRINS PRODUCTION FROM DIFFERENT STARCH SOURCES USING <i>BACILLUS MACERANS</i> CYCLODEXTRIN GLUCANOTRANSFERASE

Abstract

This study relates to the mathematical modelling of enzymatic production of Cyclodextrins (CDs) by Cyclodextrin Glucanotransferase (CGTase) from <i>Bacillus macerans</i>. The experiments were carried out in batch mode using different starch sources and the results were used to estimate unknown parameters using linearization and dynamic simulation methods. α- and β-CD produced from tapioca were found to give the highest Michaelis-Menten constant, K_M,i of 58.23 and 54.07 g L^-1, respectively and maximum velocity, V_max,i of 3.45 and 2.76 g L^-1.min, respectively, while sago resulted in the highest K_M,i and V_max,i values of 342.35 g L^-1 and 5.97 g L^-1.min, respectively, for γ-CD obtained by the linearization method. Value of product inhibition, K_1,i and CD degradation coefficient rate, δ_CD,i, were estimated using dynamic simulation, indicating that exponential reaction kinetics could be fitted better with the experimental data. Sensitivity analysis revealed that the product inhibition parameter in the exponential reaction kinetic equation is more significant in the process. For validation, the production of CDs by fed batch method was undertaken and starch and enzyme were added into the reaction medium. Then, the predicted profiles generated by simulation were compared with the experimental values. The proposed exponential reaction kinetics shows good fitting with the experimental data

Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin

“…Kelly et al ( 2008 ) reported values ranging from 3.0 × 10 −3 /s to 329/s for CGTases produced with CS as carbon source. The CGTase from B. megaterium , using AS as substrate in this work, is in agreement with the Kcat values published elsewhere (Shahrazi et al, 2013 ; Usharani et al, 2014 ). CDs are produced by the catalytic action of CGTase through an intramolecular transglycosylation reaction.…”

Development of Cellulose Nanofibre (Cnf) Derived From Kenaf Bast Fibre and It’s Potential in Enzyme Immobilization Support

“…The percentage of α-CD forming for 1 hour shows drastically increased up to 28 % of production yield and gradually increases up to 48 % after 8 hours of reaction time, as presented in Figure 2. Substrate limitation and product inhibition probably effected to the enzymatic reaction of CGTase, indicating stationary line when the reaction process time was extended [24]. Fig.…”

Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R

“…Because these molecules can form inclusion complexes with many compounds, they are widely used in the food, pharmaceutical, cosmetic, and chemical industries . In the last years, there has been a progressive increase in the number of publications and patents related to the production of CDs . Because the purification of α‐ and γ‐CDs considerably increases the cost of production, 97% of the CDs used in the market are β‐CDs .…”