Dynamic MR imaging and tumor angiogenesis in DMBA-induced rat breast cancer: Three dimensional (3D) reconstructed image analysis of tumor microvessels by confocal laser scanning microscopy

Yasumura, Kazuhiko; Ogawa, Kenji; Nakagawa, Yoichiro; Yuasa, Yuji; Hiramatsu, H; Hiramatsu, Kazuhiro; Osamura, R Y

doi:10.1007/bf02966524

Cited by 3 publications

(9 citation statements)

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“…For instance, Itoh et al (46) were able to obtain three-dimensional reconstructed images of cells in the pituitary gland and their microvascular environment using confocal laser microscopy. Yasumura et al (105) were able to determine by confocal laser microscopy microvessel densities and permeabilities in three-dimensional reconstructed images of rat mammary tumor. In the kidney, the capillary network of the glomerulus (50) and its circulation (70) have been studied.…”

Section: Three-dimensional Analysis Of Serial Sectionsmentioning

confidence: 99%

The use of microcomputed tomography to study microvasculature in small rodents

Bentley

Ortiz

Ritman

et al. 2002

American Journal of Physiology-Regulatory, Integrative and Comp

135

109

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The use of microcomputed tomography to study microvasculature in small rodents. Am J Physiol Regulatory Integrative Comp Physiol 282: R1267-R1279, 2002 10.1152/ajpregu.00560.2001.-Appropriate nephron function is dependent on the intrarenal arrangement of blood vessels. The preferred and primary means to study the architecture of intrarenal circulation has been by filling it with opaque substances such as india ink, radio-opaque contrast material, or various polymers for study by light or scanning electron microscopy. With such methodologies, superficial vessels may obscure deep vessels and little quantitative information may be obtained. Serial-section microtomy has not been practical because of problems relating to alignment and registration of adjacent sections, lost sections, and preparation time and effort. Microcomputed tomography (micro-CT) overcomes such limitations and provides a means to study the three-dimensional architecture of filled vessels within an intact rodent kidney and to obtain more quantitative information. As an example of micro-CT's capabilities, we review the use of micro-CT to study the alterations in renal microvasculature caused by the development of liver cirrhosis after chronic bile duct ligation. In this example, micro-CT evidence shows a selective decrease in cortical vascular filling in the kidney, with a maintenance of medullary vascular filling. These changes may contribute to the salt and water retention that accompanies cirrhosis. These results indicate that micro-CT is a promising method to evaluate renal vascular architecture in the intact rodent kidney relative to physiological and pathological function. kidney; imaging; microcirculation; vasculature APPROPRIATE NEPHRON FUNCTION appears to be dependent on the detailed three-dimensional interrelationship of blood vessels with the tubular components. Many techniques and/or methods have been developed to examine the microanatomic arrangement of pre-and postglomerular vasculature (4,29,36,40,54,55,59,71,98). In this respect, such efforts were prompted by early studies showing that urinary excretion varied in relation to the intrarenal environment and was dependent on the coupling of renal microcirculation and tubular components. In 1947, Trueta and colleagues (96), studying the crush syndrome in rabbits, observed that there was a shift of blood flow from the renal cortex to the medulla during hemorrhage. They suggested this shift to be the major explanation for the lack of urine flow, despite a preservation of renal blood flow (RBF). The shift of blood flow within the renal cortex from superficial short nephrons to deep long nephrons was later observed in sodium-retaining states such as cardiac insufficiency, hepatic cirrhosis, or hypovolemia (1,18,53,104). showed that changes in renal perfusion pressure, within a range (75-130 mmHg) in which RBF remains unchanged (RBF autoregulation), were followed by dramatic changes in sodium excretion. This situation is comparable to that seen in the early stages Address for reprint requ...

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Section: Three-dimensional Analysis Of Serial Sectionsmentioning

confidence: 99%

The use of microcomputed tomography to study microvasculature in small rodents

Bentley

Ortiz

Ritman

et al. 2002

American Journal of Physiology-Regulatory, Integrative and Comp

135

109

View full text Add to dashboard Cite

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“…( 28,29 ) It should be emphasized that the use of confocal laser scanning microscopy (CLSM) together with MRI may have great potential to evaluate antivascular agents in tumor regions in more detail, because the tumor vessel network and hemodynamics can be examined directly. ( 26 )…”

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“…The antivascular effect of TZT‐1027 was evaluated using MRI technique using a 1.5 Telsa system (Signa echo speed, GE Medical Systems, WI, USA), as reported previously. ( 26 ) After anesthesia with pentobarbital was initiated, the tumor‐bearing rats were placed on a phased array extremity coil designed for knee examination. A 27 G butterfly needle was inserted into the tail vein of each rat for remote injection of the contrast medium.…”

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confidence: 99%

“…The images of a subcutaneous tumor and the liver obtained by MRI were evaluated according to the following two approaches: ( 26 ) quantification through the Time‐Intensity curve (TI‐curve) of dynamic MRI and the contrast imaging/effect ratio as determined by the liver/tumor contrast noise ratio (CNR); and qualitative assay by means of positive enhancement integral images (PEIIs, color images).…”

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confidence: 99%

“…The antivascular effect of TZT‐1027 was evaluated using the CLSM technique as reported previously. ( 26 ) A fluorescent probe, FITC (fluorescein isothiocyanate, Dojindo, Japan) conjugated 20% gelatin (Sigma, St. Louis, MO, USA) solution was employed to label the microvessels. The mice bearing MX‐1 tumors were fixed on a hot plate to warm the body under anesthesia with pentobarbital, and the fluorescent probe (37°C) was injected at 1.5 mL/min for 20 min into the left ventricle of the heart in the same manner as a thoracotomy.…”

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