Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence

Grigoryev, Sergei A.; Nikitina, Tatiana; Pehrson, John R.; Singh, Prim B.; Woodcock, Christopher L.

doi:10.1242/cs.01537

Cited by 63 publications

(54 citation statements)

References 62 publications

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“…4D). Heterochromatin-associated protein HP1␣ relocalized from the chromocenters to a more diffuse nuclear staining in non-dividing cells as previously described (Grigoryev et al, 2004). All quiescence-related changes in LAP2␣ and LINT-25 were fully reversible upon readdition of serum to serum-starved cultures (data not shown).…”

supporting

confidence: 74%

“…Pericentric heterochromatin is composed mainly of long stretches of repetitive DNA sequences and is characterized by the existence of several epigenetic 'marks', including methylation of CpG dinucleotides, hypoacetylation and specific methylation of core histones and enrichment in HP1 (Maison and Almouzni, 2004). Although constitutive heterochromatin is presumed to be stable and independent of the state of cell differentiation or proliferation, recent findings have demonstrated a surprisingly dynamic feature of this type of heterochromatin in vivo during transitions between quiescent and cycling states (Baxter et al, 2004;Grigoryev et al, 2004). Repressive factors, such as HP1 and Ikaros, relocate from chromocenters in proliferating cells to take on a diffuse distribution throughout the nucleus in quiescent cells (Grigoryev et al, 2004).…”

Section: How Does Lint-25 Affect Lap2␣?mentioning

confidence: 99%

“…3) we wondered how LINT-25 upregulation relates to the chromatin reorganization reported during cell cycle exit in mouse fibroblasts (Grigoryev et al, 2004). Therefore, we analyzed LINT-25 expression and localization in primary mouse skin fibroblasts.…”

mentioning

confidence: 99%

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LAP2α-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit

Naetar

Hutter

Dorner

et al. 2007

Journal of Cell Science

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Lamina-associated polypeptide 2α (LAP2α) is a nuclear protein dynamically associating with chromatin during the cell cycle. In addition, LAP2α interacts with A-type lamins and retinoblastoma protein and regulates cell cycle progression via the E2F-Rb pathway. Using yeast two-hybrid analysis and three independent in vitro binding assays we identified a new LAP2α interaction partner of hitherto unknown functions, which we termed LINT-25. LINT-25 protein levels were upregulated during G1 phase in proliferating cells and upon cell cycle exit in quiescence, senescence and differentiation. Upon cell cycle exit LINT-25 accumulated in heterochromatin foci, and LAP2α protein levels were downregulated by proteasomal degradation. Although LAP2α was not required for the upregulation and reorganization of LINT-25 during cell cycle exit, transient expression of LINT-25 in proliferating cells caused loss of LAP2α and subsequent cell death. Our data show a role of LINT-25 and LAP2α during cell cycle exit, in which LINT-25 acts upstream of LAP2α.

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supporting

confidence: 74%

Section: How Does Lint-25 Affect Lap2␣?mentioning

confidence: 99%

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LAP2α-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit

Naetar

Hutter

Dorner

et al. 2007

Journal of Cell Science

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“…These results indicated that terminal differentiation is accompanied by a reorganization of pericentric heterochromatin. It has also been demonstrated that distribution of heterochromatin is dependent of the state of cell proliferation-diffuse nuclear staining of HP1α was characteristic for non-dividing cells (Grigoryev et al 2004;Naetar et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

Stachecka

Walczak

Kociucka

et al. 2017

Histochem Cell Biol

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for further study, a comparative characteristic of the nuclear organization in BM-MSCs and AD-MSCs was performed. Nuclear substructures were visualized by indirect immunofluorescence (nucleoli, nuclear speckles, PML bodies, lamins, and HP1α) or fluorescence in situ hybridization (telomeres) on fixed cells at 0, 3, 5, and 7 days of differentiation. Comprehensive characterization of these structures, in terms of their number, size, dynamics, and arrangement in three-dimensional space of the nucleus, was performed. It was found that during differentiation of porcine MSCs into adipocytes, changes of nuclear organization occurred and concerned: (1) the nuclear size and shape; (2) reduced lamin A/C expression; and (3) reorganization of chromocenters. Other elements of nuclear architecture such as nucleoli, SC-35 nuclear speckles, and telomeres showed no significant changes when compared to undifferentiated and mature fat cells. In addition, the presence of a low number of PML bodies was characteristic of the studied porcine mesenchymal stem cell adipogenesis system. It has been shown that the arrangement of selected components of nuclear architecture was very similar in MSCs derived from different sources, whereas adipocyte differentiation involves nuclear reorganization. This study adds new data on nuclear organization during adipogenesis using the pig as a model organism.

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“…Ces chromocentres sont visibles dans tous les types cellulaires, mais leur composition varie généralement d'un type cellulaire à un autre ainsi qu'au cours de la différenciation [19][20][21]. Ces changements peuvent être dépendants du cycle cellulaire [22,23] et sont expérimentalement réversibles. Ainsi, des expériences de reprogrammation nucléaire par fusion cellulaire (hétérokaryons) et clonage ont montré la plasticité de l'organisation nucléaire en général et des centromères en particulier qui adoptent rapidement la configuration associée à leur nouvelle identité cellulaire [24,25].…”

Section: Dynamique Transcriptionnelle De L'hétérochromatineunclassified

L’hétérochromatine constitutive dans tous ses états

Terranova

2008

Med Sci (Paris)

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Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence

Cited by 63 publications

References 62 publications

LAP2α-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit

LAP2α-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit

Nuclear organization during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

L’hétérochromatine constitutive dans tous ses états

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