Dynamic-SERS Optophysiology: A Nanosensor for Monitoring Cell Secretion Events

Lussier, Félix; Brulé, Thibault; Vishwakarma, Medhavi; Das, Tamal; Spatz, Joachim P.; Masson, Jean-François

doi:10.1021/acs.nanolett.6b01371

Cited by 117 publications

(100 citation statements)

References 47 publications

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“…[ 17–20 ] On the other hand, efforts have been made to computationally decipher the contributions of a number of analytes to the final SERS spectra. [ 21,22 ]…”

Section: Introductionmentioning

confidence: 99%

Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media

Plou

Garcı́a

Charconnet

et al. 2020

Adv Funct Materials

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The composition and intercellular interactions of tumor cells in the tissues dictate the biochemical and metabolic properties of the tumor microenvironment. The metabolic rewiring has a profound impact on the properties of the microenvironment, to an extent that monitoring such perturbations could harbor diagnostic and therapeutic relevance. A growing interest in these phenomena has inspired the development of novel technologies with sufficient sensitivity and resolution to monitor metabolic alterations in the tumor microenvironment. In this context, surface-enhanced Raman scattering (SERS) can be used for the label-free detection and imaging of diverse molecules of interest among extracellular components. Herein, the application of nanostructured plasmonic substrates comprising Au nanoparticles, self-assembled as ordered superlattices, to the precise SERS detection of selected tumor metabolites, is presented. The potential of this technology is first demonstrated through the analysis of kynurenine, a secreted immunomodulatory derivative of the tumor metabolism and the related molecules tryptophan and purine derivatives. SERS facilitates the unambiguous identification of trace metabolites and allows the multiplex detection of their characteristic fingerprints under different conditions. Finally, the effective plasmonic SERS substrate is combined with a hydrogel-based three-dimensional cancer model, which recreates the tumor microenvironment, for the real-time imaging of metabolite alterations and cytotoxic effects on tumor cells.

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“…[ 17–20 ] On the other hand, efforts have been made to computationally decipher the contributions of a number of analytes to the final SERS spectra. [ 21,22 ]…”

Section: Introductionmentioning

confidence: 99%

Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media

Plou

Garcı́a

Charconnet

et al. 2020

Adv Funct Materials

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“…SERS was also applied to detect and quantify three types of bacterial meningitis pathogens in the cerebral spinal fluid [256]. Masson group developed plasmonic nanosensors to detect metabolites that were secreted from cells, such as pyruvate, lactate, ATP, and urea [257,258], or neurotransmitters (dopamine and glutamate) [259] near living cell surface. SERS-detectors may provide quantitative estimation of the analyte or can give qualitative information regarding analyte presence in the studied medium.…”

Section: Sers-detectorsmentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

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This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

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“…127 PCA was used to extract SERS spectra of pyruvate, lactate, ATP, glucose, and urea while dynamic SERS (DSERS) was used to monitor the single molecule fluctuations of the metabolites. 127 Glucose sensing was investigated by Yonzon et al with the use of (1-mercaptoundeca-11-yl)tri(ethylene glycol) modified silver film over nanospheres (AgFON) on copper substrates. 128 A small volume flow cell was used to minimize environmental interferences at the SERS active surface.…”

Section: Metabolitesmentioning

confidence: 99%

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

Nguyen

Peters

Schultz

2017

Reviews in Analytical Chemistry

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Surface enhanced Raman scattering (SERS) has become a powerful technique for trace analysis of biomolecules. The use of SERS-tags has evolved into clinical diagnostics, the enhancement of the intrinsic signal of biomolecules on SERS active materials shows tremendous promise for the analysis of biomolecules and potential biomedical assays. The detection of the de novo signal from a wide range of biomolecules has been reported to date. In this review, we examine different classes of biomolecules for the signals observed and experimental details that enable their detection. In particular, we survey nucleic acids, amino acids, peptides, proteins, metabolites, and pathogens. The signals observed show that the interaction of the biomolecule with the enhancing nanostructure has a significant influence on the observed spectrum. Additional experiments demonstrate that internal standards can correct for intensity fluctuations and provide quantitative analysis. Experimental methods that control the interaction at the surface are providing for reproducible SERS signals. Results suggest that combining advances in methodology with the development of libraries for SERS spectra may enable the characterization of biomolecules complementary to other existing methods.

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Dynamic-SERS Optophysiology: A Nanosensor for Monitoring Cell Secretion Events

Cited by 117 publications

References 47 publications

Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media

Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media

Raman Scattering: From Structural Biology to Medical Applications

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

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