Dynamic thiol–disulfide homeostasis in acute ischemic stroke patients

Abstract

Dynamic thiol-disulfide homeostasis plays a critical role in the cellular protection provided by antioxidation. The aim of this study was to investigate whether there is a change in thiol-disulfide homeostasis in acute ischemic stroke patients. Patients diagnosed with acute ischemic stroke that had undergone magnetic resonance diffusion-weighted imaging within the first 24 h were prospectively included in this study. The thiol, disulfide, and total thiol levels were measured during the first 24 and 72 h, and the National Institutes of Health Stroke Scale (NIHSS), modified Rankin Scale (mRS), and Barthel Index (BI) of the patients were recorded. Overall, the relationships between the thiol-disulfide levels of the patients and the infarct volumes, NIHSS, mRS, and BI scores were investigated. In this study, 54 patients and 53 healthy controls were included. The mean of the native thiol levels in the stroke group was 356.572 ± 61.659 μmol/L (min/max 228.00/546.40), while it was 415.453 ± 39.436 μmol/L (min/max 323.50/488.70) in the control group (p< 0.001). A negative, significant correlation was observed between the infarct volumes and native thiol levels (ρ = -0.378; p = 0.005), and the disulfide levels were similar between the groups (Z = 0.774; p = 0.439). Significant difference was found between the thiol levels of the mild and moderate-severe NIHSS groups (p = 0.026). The changes in the thiol levels under oxidative stress may be associated with the severity of the stroke. Substitution of thiol deficiency and correction of thiol-disulfide imbalance may be beneficial in ischemic stroke.

Evaluation of Dynamic Disulphide/Thiol Homeostasis in Silica Exposed Workers

“…Thiol groups are oxidized to disulphides during oxidative stress, thus thiol/disulphide ratio offers a simple and convenient expression of cellular oxidative stress (30). Recently, thiol disulphide homeostasis has been investigated in many disorders, such as acute myocardial infarction, type 1 diabetes and acute ischaemic stroke (31,32,33). …”

Oxidative injury associated with stenting of asymptomatic carotid artery stenosis

“…Previous studies have shown that serum thiol levels are lower in acute ischaemic stroke patients compared to healthy patients; with an increase in the diameter of the arteries causing the ischaemia, thiol levels decrease further [22][23][24]. Patients with lower thiol levels using diff erent neurological assessment scales (NIHSS, mRS and Barthel scale etc.)…”

Oxidative Stress and Antioxidant Markers in Serum and Red Blood Cell of Patients with Ischemic and Hemorrhagic Stroke: A Case-Control Study

“…Tsai et al (24) reported that the amount of free thiols in acute stroke patients increased by the seventh day to a level similar to that of the control group. Several studies reported that the thiol level in IS patients was significantly lower than in the control (4,24,25). Thiols are organic compounds that contain a sulfhydryl (-SH) group that protects against oxidative stress.…”

“…Bektas et al (25) proposed that thiol/disulfide hemostat is impaired in IS patients and, as the thiol level decreases any medicinal drug that contains the sulfhydryl group can increase H2S levels. Furthermore, H2S is itself is associated with vasodilatation.…”