Dynamic Visualization of Local Protein Synthesis in Hippocampal Neurons

Aakalu, Girish; Smith, W. Bryan; Nguyen, Nhien Thi; Jiang, Chenfanfu; Schuman, Erin M.

doi:10.1016/s0896-6273(01)00295-1

Cited by 548 publications

(531 citation statements)

References 42 publications

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“…3A.2). Interestingly, they observed that the fluorescently labeled Kv1.1 was concentrated in stationary "hotspots" in dendrites similar to what was reported with other locally translated protein (Aakalu et al 2001).…”

Section: Single-cell Imagingsupporting

confidence: 79%

“…Schuman and colleagues created a dendritic translation reporter by flanking the coding sequence for GFP with the 5 0 and 3 0 untranslated regions (UTR) of the a subunit of Ca 2þ /calmodulindependent kinase II (aCaMKII) (Fig. 3A.1) (Aakalu et al 2001;Sutton et al 2004). The 3 0 UTR of aCaMKII has been shown to be sufficient for dendritic localization of the mRNA in neurons.…”

Section: Fluorescent Protein Reportersmentioning

confidence: 99%

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Imaging Translation in Single Cells Using Fluorescent Microscopy

Chao

Yoon

Singer

2012

Cold Spring Harbor Perspectives in Biology

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The regulation of translation provides a mechanism to control not only the abundance of proteins, but also the precise time and subcellular location that they are synthesized. Much of what is known concerning the molecular basis for translational control has been gleaned from experiments (e.g., luciferase assays and polysome analysis) that measure average changes in the protein synthesis of a population of cells, however, mechanistic insights can be obscured in ensemble measurements. The development of fluorescent microscopy techniques and reagents has allowed translation to be studied within its cellular context. Here we highlight recent methodologies that can be used to study global changes in protein synthesis or regulation of specific mRNAs in single cells. Imaging of translation has provided direct evidence for local translation of mRNAs at synapses in neurons and will become an important tool for studying translational control.

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Section: Single-cell Imagingsupporting

confidence: 79%

Section: Fluorescent Protein Reportersmentioning

confidence: 99%

Imaging Translation in Single Cells Using Fluorescent Microscopy

Chao

Yoon

Singer

2012

Cold Spring Harbor Perspectives in Biology

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“…Indeed, electron and confocal microscopy experiments indicate that local protein synthesis could be even restricted to individual synaptic spines (Aakalu et al, 2001;Ostroff et al, 2002). Furthermore, the transient activation of local protein synthesis is a particularly well-suited mechanism for coupling tagging with de novo transcription at the cell nucleus.…”

Section: B Regulation Of Local Protein Synthesismentioning

confidence: 99%

Synapse-specific stabilization of plasticity processes: The synaptic tagging and capture hypothesis revisited 10 years later

Barco

Armentia

Alarcón

2008

Neuroscience & Biobehavioral Reviews

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Synapse-specific stabilization of plasticity processes: The synaptic tagging and capture hypothesis revisited ten years later, Neuroscience and Biobehavioral Reviews (2008Reviews ( ), doi:10.1016Reviews ( /j.neubiorev.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d m a n u s c r i p t A c c e p t e d m a n u s c r i p tThe STC hypothesis revisited 2 Abstract A decade ago, the synaptic tagging hypothesis was proposed to explain how newly synthesized plasticity products can be specifically targeted to active synapses. A growing number of studies have validated the seminal findings that gave rise to this model, as well as contributed to unveil and expand the range of mechanisms underlying late-associativity and neuronal computation.Here, we will review what it was learnt during this past decade regarding the cellular and molecular mechanisms underlying synaptic tagging and synaptic capture. The accumulated experimental evidence has widened the theoretical framework set by the synaptic tagging and capture (STC) model and introduced concepts that were originally considered part of alternative models for explaining synapse-specific LTP. As a result, we believe that the STC model, now improved and expanded with these new ideas and concepts, still represents the most compelling hypothesis to explain late-associativity in synapse-specific plasticity processes. We will also discuss the impact of this model in our view of the integrative capability of neurons and associative learning. wordsKeywords: synaptic plasticity; LTP; associative learning; neuronal computation; synaptic tagging; synaptic capture; metaplasticity A c c e p t e d m a n u s c r i p tThe STC hypothesis revisited 3 Contents

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“…An elegant study using the arsenic-based dyes FlAsH and ReAsH showed that transfected tagged GluR1 and GluR2 subunits can be synthesized in a dendritic compartment independent from the cell body, in cultured hippocampal neurons (Ju et al, 2004). Studies using a protein synthesis reporter consisting of GFP flanked by the 5= and 3= untranslated regions from the CaMKII ␣-subunit, which contains information sufficient for the dendritic localization of the mRNA, also showed that BDNF may induce dendritic protein synthesis (Aakalu et al, 2001). The neurotrophin BDNF plays an important role in synaptic plasticity (Carvalho et al, 2008), and studies in hyppocampal synaptoneurosomes showed that BDNF induces local synthesis of GluR1 (Schratt et al, 2004).…”

Section: Role Of Ampars In Ltpmentioning

confidence: 99%