Through direct visualization at the discrete level, the microexcitations in cold 2 + 1D dusty plasma liquids formed by negatively charged dusts suspended in low pressure gaseous discharges were experimentally investigated, in which the downward ion flow wake field induces strong vertical coupling and chain bundle structure. It is found that the horizontal structure and motion are similar to those of the two-dimensional liquid. Different types of basic cooperative chain excitations: straight vertical chains with small amplitude jittering, chain tilting-restraightening, bundle twisting-restraightening, and chain breaking-reconnection, are observed. The region with good ͑poor͒ horizontal structural order prefers the straight ͑tilted or broken͒ chains with little ͑large͒ titling and tilting rate.