Dynamics and Predictive Potential of Antibodies against Insect-Derived Recombinant Leishmania infantum Proteins during Chemotherapy of Naturally Infected Dogs

Todolí, Felicitat; Galindo, Inmaculada; Gómez-Sebastián, Silvia; Pérez-Filgueira, Mariano; Escribano, José M.; Alberola, Jordi; Rodríguez-Cortés, Alhelí

doi:10.4269/ajtmh.2010.09-0147

Cited by 6 publications

(1 citation statement)

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“…As an alternative, the use of lepidoptera larvae has been reported as a suitable method for inexpensive production of large amounts of recombinant proteins. Moreover, previous studies 34, [40][41][42] show that crude protein extracts without further purification can be used as immunodiagnostic reagents, because the recombinant proteins preserve their antigenic activities. However, some interference of insect proteins, such as cross-reactions, cannot be ruled out, especially in dogs with a positive DTH response against Ni proteins.…”

Section: Discussionmentioning

confidence: 99%

Humoral and In Vivo Cellular Immunity against the Raw Insect-Derived Recombinant Leishmania infantum Antigens KMPII, TRYP, LACK, and papLe22 in Dogs from an Endemic Area

Todolí

Solano‐Gallego²,

Juan³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Leishmania infantum causes visceral leishmaniasis, a severe zoonotic and systemic disease that is fatal if left untreated. Identification of the antigens involved in Leishmania -specific protective immune response is a research priority for the development of effective control measures. For this purpose, we evaluated, in 27 dogs from an enzootic zone, specific humoral and cellular immune response by delayed-type hypersensitivity (DTH) skin test both against total L. infantum antigen and the raw Trichoplusia ni insect-derived kinetoplastid membrane protein-11 (rKMPII), tryparedoxin peroxidase (rTRYP), Leishmania homologue of receptors for activated C kinase (rLACK), and 22-kDa potentially aggravating protein of Leishmania (rpapLe22) antigens from this parasite. rTRYP induced the highest number of positive DTH responses (55% of leishmanin skin test [LST]-positive dogs), showing that TRYP antigen is an important T cell immunogen, and it could be a promising vaccine candidate against this disease. When TRYP-DTH and KMPII-DTH tests were evaluated in parallel, 82% of LST-positive dogs were detected, suggesting that both antigens could be considered as components of a standardized DTH immunodiagnostic tool for dogs.

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Section: Discussionmentioning

confidence: 99%