Dynamics of Bacterial Community Composition and Activity during a Mesocosm Diatom Bloom

Riemann, Lasse; Steward, Grieg F.; Azam, Farooq

doi:10.1128/aem.66.5.2282-2282.2000

Cited by 213 publications

(364 citation statements)

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“…In the previous analysis of the bulk sediment of Lake Biwa, no sequence of this lineage was detected as a major DGGE band (16). The isolated members of Bacteroidetes catabolize various polymeric substances, and several culture-independent studies have demonstrated the association of Bacteroidetes with the degradation of complex organic materials (4, 25,26). The bacteria detected here may be utilizing sheath material as a substrate.…”

Section: Phylogenetic Affiliations Of Sheath-associated Bacteriamentioning

confidence: 53%

Community Structure of Bacteria Associated with Sheaths of Freshwater and Brackish Thioploca Species

2006

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Bacterial communities associated with sheaths of Thioploca spp. from two freshwater lakes (Lake Biwa, Japan, and Lake Constance, Germany) and one brackish lake (Lake Ogawara, Japan) were analyzed with denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. The comparison between the DGGE band patterns of bulk sediment and Thioploca filaments of Lake Biwa suggested the presence of specific bacterial communities associated with Thioploca sheaths. As members of sheath-associated communities, bacteria belonging to Bacteroidetes were detected from the samples of both freshwater lakes. A DGGE band from Thioploca of Lake Biwa, belonging to candidate division OP8, was quite closely related to another DGGE band detected from that of Lake Constance. In contrast to the case of freshwater lakes, no bacterium of Bacteroidetes or OP8 was detected from Thioploca of Lake Ogawara.However, two DGGE bands from Lake Ogawara, belonging to Chloroflexi, were quite closely related to a DGGE band from Lake Constance. Two DGGE bands obtained from Lake Biwa were closely related to phylogenetically distant dissimilatory

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Section: Phylogenetic Affiliations Of Sheath-associated Bacteriamentioning

confidence: 53%

Community Structure of Bacteria Associated with Sheaths of Freshwater and Brackish Thioploca Species

2006

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“…They are known to be chemoorganotrophic particle colonisers and especially proficient in degrading various polymers such as cellulose, chitin and pectin, but also high-molecular DOM (DeLong et al 1993;Riemann et al 2000;Riemann et al 2006;Kirchman 2002;Cottrell and Kirchman 2000b). In our study, the majority of bacteroidetes phylotypes belong to the Flavobacteriaceae with eight phylotypes related to Polaribacter spp.…”

Section: Discussionmentioning

confidence: 88%

The microbiome of North Sea copepods

et al. 2013

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Copepods can be associated with different kinds and different numbers of bacteria. This was already shown in the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community of four frequently occurring copepod species, Acartia sp., Temora longicornis, Centropages sp. and Calanus helgolandicus from Helgoland Roads (North Sea) over a period of 2 years using DGGE (denaturing gradient gel electrophoresis) and subsequent sequencing of 16S-rDNA fragments. To complement the PCR-DGGE analyses, clone libraries of copepod samples from June 2007 to 208 were generated. Based on the DGGE banding patterns of the two years survey, we found no significant differences between the communities of distinct copepod species, nor did we find any seasonality. Overall, we identified 67 phylotypes ([97 % similarity) falling into the bacterial phyla of Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The most abundant phylotypes were affiliated to the Alphaproteobacteria. In comparison with PCR-DGGE and clone libraries, phylotypes of the Gammaproteobacteria dominated the clone libraries, whereas Alphaproteobacteria were most abundant in the PCR-DGGE analyses.

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“…Other examples are bioassays to study potential limiting factors, such as temperature or organic and inorganic nutrients (Kirchman 1990). It is important to differentiate these experiments from those designed to investigate how prokaryotic assemblage composition responds to factors such as algal blooms (Riemann et al 2000) or different types of grazers (Simek et al 1999). In the latter cases, the experimental variable is phylogenetic composition, whereas in the former cases the variable is metabolic activity.…”

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confidence: 99%

Changes in marine bacterioplankton phylogenetic composition during incubations designed to measure biogeochemically significant parameters

Massana

Pedrós‐Alió

Casamayor

et al. 2001

Limnology & Oceanography

164

148

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Bottle incubations, during which the activity and growth of prokaryotes is monitored during several days, are frequently carried out to study functional aspects of marine prokaryotic assemblages. These experiments will relate directly to in situ activities if all populations grow harmonically during the incubation. We tested whether this was the case by analyzing the composition of bacterial assemblages at the beginning and at the end of the incubation by denaturing gradient gel electrophoresis. Five experiments were done in different Antarctic regions. Bacterial assemblages north and south of the Polar Front were very different. In all cases, the final assemblages were very different from the initial ones, and these changes were often accompanied by a significant decrease of diversity indices. Our experiments included treatments with different temperature and organic matter amendments. Whereas the increase in temperature tested had a minor effect on prokaryotic growth rate and specific composition, the addition of organic matter strongly stimulated growth rate and selected a particular bacterial assemblage in some experiments but not in others. A significant component of bacterial assemblages from waters south of the Polar Front appeared to be Polaribacter franzmannii, a gas vacuolated bacterium of the CytophagaߚFlavobacteriumߚBacteroides group that was originally isolated from Antarctic sea ice. This phylotype was enriched and dominated in almost all final assemblages. Our results indicate that longߚterm bottle incubations mostly measure the activity of a few opportunistic bacteria and not that of the original assemblage. This should be taken into account if data obtained in these experiments are used for balancing whole ecosystem carbon budgets and to derive biogeochemical conclusions.

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Dynamics of Bacterial Community Composition and Activity during a Mesocosm Diatom Bloom

Cited by 213 publications

References 0 publications

Community Structure of Bacteria Associated with Sheaths of Freshwater and Brackish Thioploca Species

Community Structure of Bacteria Associated with Sheaths of Freshwater and Brackish Thioploca Species

The microbiome of North Sea copepods

Changes in marine bacterioplankton phylogenetic composition during incubations designed to measure biogeochemically significant parameters

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