Dynamics of Changes in Metabolic and Hematological Parameters after Transplantation of Pancreatic Islet Cells on TiNi Scaffold in Experimental Diabetes Mellitus

Abstract

The dynamics of the development of the pancreatic islet cells in the porous TiNi scaffold was studied by electron microscopy. Changes in the metabolism of glucose and glycosylated hemoglobin and parameters of the peripheral blood and bone marrow were shown after transplantation of pancreatic islet cells on porous permeable TiNi scaffold during alloxan-induced diabetes. The cells administered on the porous biocompatible scaffold produced more prolonged anti-diabetic effect and normalized hemopoiesis parameters in comparison with their intraperitoneal administration. The experiment on pancreatic islet cells showed that porous permeable TiNi scaffold is a unique cell incubator acceptable for usage for tissue engineering.

The emerging field of pancreatic tissue engineering: A systematic review and evidence map of scaffold materials and scaffolding techniques for insulin-secreting cells

“…Scaffold size, mechanical stability, and diffusion distances affect the choice further. Among the in vivo studies included (n = 91) the ISC/scaffold construct was transplanted into subcutaneous locations (n = 31), 27,51,69,92–94,112,115,120,133,135,145,147,156,157,171,212–225 the peritoneal cavity (n = 19), 18,26,56,63,65–68,101,134,135,173,195,226–232 an omental pouch (n = 14), 57,59,81,118,119,163,174,225,233–238 the renal capsule (n = 18), 54,…”

“…Scaffold size, mechanical stability, and diffusion distances affect the choice further. Among the in vivo studies included (n = 91) the ISC/scaffold construct was transplanted into subcutaneous locations (n = 31), 27,51,69,[92][93][94]112,115,120,133,135,145,147,156,157,171,[212][213][214][215][216][217][218][219][220][221][222][223][224][225] the peritoneal cavity (n = 19), 18,26,56,63,[65][66][67][68]101,134,135,173,195,[226][227][228][229][230][231]…”

Biomaterial-mediated strategies for accurate and convenient diagnosis, and effective treatment of diabetes: advantages, current progress and future perspectives

“…In vivo tests showed that islet cells with the scaffold could significantly reduce the blood glucose level and decrease the concentration of glycosylated hemoglobin compared to intraperitoneal administration of pancreatic islet cells, indicating that the TiNi scaffold could provide a better microenvironment for islet cells to promote insulin secretion, which consequently achieved better blood glucose control. 132 Roshanbinfar et al encapsulated islet b cells in alginate microcapsules. Compared with the islet b cells without alginate encapsulation, the cells in alginate microcapsules demonstrated significantly higher viability, revealing that alginate encapsulation had good biocompatibility and nutrient availability.…”