Dynamics of changes in the genome of callus tissues of Rauwolfia serpentina upon a switch to conditions of submerged cultivation

Abstract

It is established that switching from surface to submerged cultivation in a liquid medium of a special composition does not induce noticeable changes in the callus tissues of Rauwolfia serpentina in the course of the first several passages. Polymorphism of the RAPD spectra is discovered following 4-6 growth passages of tissues in the submerged culture; this polymorphism may reflect both changes in the DNA sequence and in the genetic structure of the cell population forming the strain. Addition of an intermediate passage in a solid medium of simpler composition prior to a switch to the liquid medium does not exert a substantial influence on the level and nature of the changes in the genome.

Comprehensive study of hormone-independent highly productive strain of Rauvolfia serpentina tissue culture as a source of indole alkaloids

“…Cell biomass of the K-27M strain R. serpentina tissue culture derived by the cell selection methods from the K-27 strain, which was maintained in vitro for more than 35 years, was studied. The details of establishment and charac teristics of the initial K-27 strain were published earlier [3,[16][17][18][19][20][21][22][23][24][25][26][27][28]. The newly established K-27M strain was cultured under thermostatic conditions at 27-28 °C in the dark, in 370 mL glass vessels.…”

“…The genealogy of the К-27 strain of R. serpentina, the details of its establishment and culturing along with the characteristics and peculiarities were reported earlier [3,[7][8][9]. Over more than 35 years, this strain was stable in terms of both productivity, genetic and cytological characteristics, being grown on both liquid and solid media of various composition with or without different organic additives [16,17,26,[18][19][20][21][22][23][24][25].…”