Dynamics of heart differentiation, visualized utilizing heart enhancer elements of the Drosophila melanogaster bHLH transcription factor Hand

Sellin, Julia; Albrecht, Stefanie; Kölsch, Verena; Paululat, Achim

doi:10.1016/j.modgep.2005.09.012

Cited by 97 publications

(117 citation statements)

References 56 publications

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“…We have previously generated several transgenic Drosophila strains carrying a GFP reporter gene driven by the heart-specific enhancer element of the hand gene (Sellin et al, 2006). One of these transgenic lines (handC-GFP1.1) attracted our attention because 100% of the homozygous (but 0% of the heterozygous) viable adults failed to climb on the vertical walls of the breeding vials, indicating that the transposon integration might have caused a mutation in a climbing relevant gene (see supplementary material Movie 1).…”

Section: An Adhesion Defective Mutant Identified Upon Generation Of Tmentioning

confidence: 99%

“…The climbing mutant (handC-GFP1.1) was discovered upon generation of transgenic fly lines carrying a green fluorescent protein (GFP) reporter (Sellin et al, 2006). We obtained several independent transgenic lines carrying the same construct at different chromosomal locations but none of them displayed climbing disability on smooth vertical surfaces except for the line handC-GFP1.1.…”

Section: Molecular Characterization Of the Climbing Disabled Mutantmentioning

confidence: 99%

“…The handC-GFP1.1 reporter line was generated previously; it carries the complete third intron of the gene hand and drives GFP in, for example, cardiac tissues (Sellin et al, 2006;Popichenko et al, 2007). As this P-element insertion causes a mutation of the Su(z)2 gene (this work), we consider the handC-GFP1.1 line to be a Su(z)2 allele and renamed it Su(z)2 handC-GFP1.…”

Section: Stocksmentioning

confidence: 99%

See 2 more Smart Citations

Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2

Hüsken

Hufnagel

Mende

et al. 2015

Journal of Experimental Biology

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The ability of many insects to walk on vertical smooth surfaces such as glass or even on the ceiling has fascinated biologists for ages and has led to the discovery of highly specialized adhesive organs located at the distal end of the animals' legs. So far, research has primarily focused on structural and ultrastructural investigations leading to a deeper understanding of adhesive organ functionality and to the development of new bioinspired materials. Genetic approaches, e.g. the analysis of mutants, to achieve a better understanding of adhesive organ differentiation, have not been used so far. Here, we describe the first Drosophila melanogaster mutant that develops malformed adhesive organs, resulting in a complete loss of climbing ability on vertical smooth surfaces. Interestingly, these mutants fail to make close contact between the setal tips and the smooth surface, a crucial condition for wet adhesion mediated by capillary forces. Instead, these flies walk solely on their claws. Moreover, we were able to show that the mutation is caused by a P-element insertion into the Su(z)2 gene locus. Remobilization of the P-element restores climbing ability. Furthermore, we provide evidence that the P-element insertion results in an artificial Su(z)2 transcript, which most likely causes a gain of function mutation. We presume that this transcript causes deregulation of yet unknown target genes involved in pulvilli differentiation. Our results nicely demonstrate that the genetically treatable model organism Drosophila is highly suitable for future investigations on adhesive organ differentiation.

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Section: An Adhesion Defective Mutant Identified Upon Generation Of Tmentioning

confidence: 99%

Section: Molecular Characterization Of the Climbing Disabled Mutantmentioning

confidence: 99%

Section: Stocksmentioning

confidence: 99%

See 1 more Smart Citation

Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2

Hüsken

Hufnagel

Mende

et al. 2015

Journal of Experimental Biology

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“…A number of factors that are required for the specification and the differentiation of the different cardiac cell subtypes could be candidate cofactors to modulate Hox activity. 33,38,39,41,42,[74][75][76][77][78][79][80][81][82] (6) At metamorphosis, the activation of the ecdysone-induced signaling pathway produces some changes in the Tin-CMs which are in turn responsible for the switch of abdA activity: AbdA switches from an activator to a repressor, at least regarding Ih and Ndae1 transcription. Knowing the signal, the ecdysone, responsible for the Hox activity modification should facilitate the search of candidate factors involved in this ecdysone-dependent change of abdA activity.…”

Section: A Steroid-dependent Modification Of Hox Activities Drives Thmentioning

confidence: 99%

Downstream of Homeotic Genes: In the Heart of Hox function

Monier

Tevy²,

Perrin³

et al. 2007

Fly

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“…Directionality of haemolymph flow within the Drosophila larval heart tube is regulated by two means: (i) a directional contraction wave running along the heart tube, and (ii) an intracardiac valve cell that separates the posterior heart chamber from the aorta portion (Lehmacher et al, 2012;Miller, 1950;Molina and Cripps, 2001;Rizki, 1978;Sellin et al, 2006;Wu and Sato, 2008;Zeitouni et al, 2007;present study). Experimental data on how the valves act and on the extent to which they contribute to the control of flow properties within the heart are not yet available.…”

Section: Introductionmentioning

confidence: 99%

Formation and function of intracardiac valve cells in the Drosophila heart

Lammers

Abeln

Hüsken

et al. 2017

Journal of Experimental Biology

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Drosophila harbours a simple tubular heart that ensures haemolymph circulation within the body. The heart is built by a few different cell types, including cardiomyocytes that define the luminal heart channel and ostia cells that constitute openings in the heart wall allowing haemolymph to enter the heart chamber. Regulation of flow directionality within a tube, such as blood flow in arteries or insect haemolymph within the heart lumen, requires a dedicated gate, valve or flap-like structure that prevents backflow of fluids. In the Drosophila heart, intracardiac valves provide this directionality of haemolymph streaming, with one valve being present in larvae and three valves in the adult fly. Each valve is built by two specialised cardiomyocytes that exhibit a unique histology. We found that the capacity to open and close the heart lumen relies on a unique myofibrillar setting as well as on the presence of large membranous vesicles. These vesicles are of endocytic origin and probably represent unique organelles of valve cells. Moreover, we characterised the working mode of the cells in real time. Valve cells exhibit a highly flexible shape and, during each heartbeat, oscillating shape changes result in closing and opening of the heart channel. Finally, we identified a set of novel valve cell markers useful for future in-depth analyses of cell differentiation in wild-type and mutant animals.

show abstract

Dynamics of heart differentiation, visualized utilizing heart enhancer elements of the Drosophila melanogaster bHLH transcription factor Hand

Cited by 97 publications

References 56 publications

Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2

Adhesive pad differentiation in Drosophila melanogaster depends on the Polycomb group gene Su(z)2

Downstream of Homeotic Genes: In the Heart of Hox function

Formation and function of intracardiac valve cells in the Drosophila heart

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