Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

Abstract

<jats:p>The body of broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P &lt; 0.05) and albumin – by 17.0 % (P &lt; 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P &lt; 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.</jats:p>

Erythron and protein system in piglets blood under stress influence

“…An increase in the number of bifidobacteria, which is the basis of the perineal and cavity microflora, led to an increase in the vitality of vitamin products B6 and B 12 in process of vitality, which had a positive effect on the intensity of oxidative-reducing processes in the system of erythron and hemopoiesis of piglets in the S group. Adaptation of the system of protein metabolism in animals of this group was shown by an increase in the synthetic function of hepatocytes, transport and immune function of the blood, due to the basic properties of amber acid, as the main component of the "Biovir" (Stoyanovskyy et al, 2020). It is a universal biogenic metabolism stimulator in cells, that is, the natural endogenous substrate of oxidation-reduction processes (Krebs cycle) without additional energy expenditure, which leads to the activation of biosynthesis of proteins at different levels (Roman et al, 2020).…”

“…The use of the additive was positive for leveling changes in the protein metabolism system due to the development of an adaptive syndrome, which was manifested by an increase in the content of total protein by 9.4%, βglobulins -by 18.3 % (P < 0.05), γ-globulins -by 27,2-26.8 % (P < 0.05) compared with control. Our previous studies (Stoyanovskyy et al, 2020) showed that peptides of cell walls of lactobacillus and bifidobacteria supplements of "Biovir" had immunostimulatory properties for lymphoid tissue of the intestinal mucosa of piglets and served as substrate for the growth and development of its microbiocenosis. An increase in the number of bifidobacteria, which is the basis of the perineal and cavity microflora, led to an increase in the vitality of vitamin products B6 and B 12 in process of vitality, which had a positive effect on the intensity of oxidative-reducing processes in the system of erythron and hemopoiesis of piglets in the S group.…”

Overview: prevalence and structure of metabolic diseases of laying chickens, their influence on egg quality and condition of young chickens

“…Виникнення метаболічних хвороб пов'язано з якістю та режимом годівлі птиці на великих промислових комплексах (Yousefi et al, 2005;Vodolazhchenko, 2011;Verner, 2013;Novozhylova, 2014). Використання для годівлі птиці різноманітних сорбентів, пре-та пробіотиків, біогенних стимуляторів росту, біологічно активних речовин стало невід'ємною складовою рецептур комбікормів (Leeson, 1999;Mashkin, 2010;Stoyanovskyy et al, 2020;Yaremchuk et al, 2020). Проте навіть такі заходи не убезпечують птицю від метаболічних хвороб.…”

The Intensity of Lipid Peroxide Oxidation Processes and the System State of Antioxidant Protection of Broiler Chicken Due to the Action of the Synbiotic Preparation in Complex with the Disinfectant

“…It became known that probiotics can modulate the redox status of the recipient due to their ability to chelate metal ions, and antioxidant systems, thus regulating signaling pathways and enzymes that produce reactive forms of oxygen and intestinal microbiota. 10,11 There is scientific interest in finding potential probiotic strains that may exhibit powerful antioxidant properties along with health benefits. In vitro and in vivo research have ascertained that probiotics exhibit antioxidant potential.…”