Dynamics of Ventricular Wall and Interventricular Septum Thickness of Rat’s Heart in the Early Postnatal Period in Normal Conditions and after Intranatal Injection of Dexamethasone

Hryhorieva, O. A.; Cherniavskyi, A. V.

doi:10.26693/jmbs03.03.012

Cited by 3 publications

(2 citation statements)

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“…The sheep, as an experimental animal in this area of research, is often used as a model used in biomedical research and is perhaps one of the most influential models of human organ systems. At the same time, morphometric studies of organs and systems in clinically healthy animals are a priority for timely and reliable diagnosis of diseases of various geneses, which are diagnostic criteria as indicators of normalcy for the diagnosis of diseases of contagious and non-contagious pathology ( Hryhorieva & Cherniavskyi, 2018a;Shevchenko, 2018). The mathematical analysis of the structures of morphological objects has been recognized as a modern method that is distinguished by objectivity and reliability, which allows for a deeper understanding of the development of the pathological process and logical interpretation of the results of scientific research D. Baldridge et al (2021) and I.…”

Section: Introductionmentioning

confidence: 99%

Peculiarities of organometry and morphoarchitectonics of the heart of the Domestic ram (Ovis aries L., 1758)

Horalskyi,

Ragulya,

Kolesnik

et al. 2023

Ukrainian journal of veterinary sciences

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Today, cardiovascular diseases are the dominant diseases of animals, which most often lead to their death both in Ukraine and worldwide. Therefore, morphological studies of the heart at the cellular, tissue, and organ levels are essential in clinical cardiology for in vivo ultrasound diagnosis of functional and organic heart lesions. The aim of the study was to perform a histological and cytomorphometric assessment of the morphological structures of the sheep heart using macroscopic, histological, and morphometric methods. Using morphological methods, the hearts of sexually mature clinically healthy animals (n=5) belonging to the class Mammalia – Mammals, species Ovis aries L., 1758 – domestic sheep (ram) were studied. The absolute and relative heart weights of mature sheep were determined, which are, respectively, 208.4±9.82 g and 0.44±0.007%, and the weight without epicardial fat is 175.0±8.17 g. The heart of sheep, according to the development index (145.5±4.02%), belongs to the expanded-shortened anatomical type. The most developed components are the ventricles. At the same time, the ratio of the mass of the heart ventricles to its net mass is 1:0.78, respectively, the ratio of the mass of the atria is 1:0.22, and the ratio of the mass of the atrial myocardium to the mass of the ventricular myocardium is 1:0.29. The largest volume was found in the cardiomyocytes of the left ventricle – 3982.99±423.96 μm3 , the smaller – in the right ventricle – 2463.02±318.04 μm3 and the smallest – in the atrial cardiomyocytes – 1215.93±176.94 μm3 . The volumes of cardiomyocyte nuclei in the left (53.42±5.18 μm3 ), right (52.85±4.33 μm3 ) ventricles and atria (50.16±4.57 μm3 ) were almost identical. Nuclear-cytoplasmic ratio was the lowest in cardiomyocytes of the left ventricle – 0.0136±0.0062, slightly higher in cardiomyocytes of the right ventricle – 0.0219±0.0079 and the highest – 0.0430±0.0096 in atrial cardiomyocytes. The obtained results on the macroand microscopic structure of the heart of bighorn sheep (Ovis aries L., 1758) complement the information on the morphology of the sheep heart in the relevant sections of histology and comparative anatomy and are necessary for clinical cardiology

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Section: Introductionmentioning

confidence: 99%

Peculiarities of organometry and morphoarchitectonics of the heart of the Domestic ram (Ovis aries L., 1758)

Horalskyi,

Ragulya,

Kolesnik

et al. 2023

Ukrainian journal of veterinary sciences

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Section: Introductionmentioning

confidence: 99%

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2023

Ukrainian journal of veterinary sciences

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Morphometric parameters of the heart of domestic sheep Ovis aries L., 1758

Ragulya,

Goralskyi,

Sokulskyi

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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The cardiovascular system is one of the most critical animal systems. Its main functions are to supply organs and tissues with oxygen and nutrients and to remove metabolic products from tissues. Diseases of the cardiovascular system of animals cause significant economic damage, including reduced productivity, reproductive qualities, and the development of concomitant diseases. The prevention, diagnosis, surgery, and treatment of such pathologies are only possible by knowing the morphofunctional parameters of comparative anatomy, histology, and physiology. The heart plays a vital role in blood circulation and regulates the proper functioning and development of all organs of animal and human organisms. The study of the heart structure reveals topography features, age-related morphology, and development of this organ in domestic animals and remains relevant. The article is a fragment of the scientific developments of the Department of Normal and Pathological Morphology, Hygiene and Forensics of Polissia National University on the following topics: “Features of the morphology of the heart of domestic mammals” (state registration number 0121U108884); “Development, morphology and histochemistry of animal organs in normal and pathological conditions”, state registration number 0113U000900. In the study, sexually mature clinically healthy animals (n = 5) belonging to the class Mammalia – Mammals, species Ovis aries L., 1758 – domestic sheep (ram) were investigated. The study aimed to evaluate the morphological structures of the heart of mature sheep using macro- and microscopic, morphometric, and statistical research methods. The heart of a sexually mature domestic sheep was subjected to anatomical dissection. Sections were stained with hematoxylin and eosin to study the cyto- and histoarchitectonics of the heart for microscopic examination of transverse striated myocardial muscle tissue, detection of cardiomyocyte cytostructure, and Heidenhain staining of histological specimens were used. The morphology of the heart in sheep has a similar organization plan, topographic location of the organ, and anatomical and histological structure. However, there are some striking species and morphological features. The heart of the domestic sheep belongs to the expanded-shortened anatomical type (according to its development index – 145.5 ± 4.02 %). According to the studies, the absolute and relative weight of the heart of mature sheep is, accordingly, 208.4 ± 9.82 g and 0.44 ± 0.007 %, and the weight without epicardial fat is 175.0 ± 8.17 g. It has been shown that the microscopic structure of the ventricles and atria of the sheep heart differ in cytometric parameters. Cardiomyocytes of the left ventricle have the most significant volume (3982.99 ± 423.96 μm3), the smaller – of the right ventricle (2463.02 ± 318.04 μm3). The lowest index was observed in atrial cardiomyocytes (1215.93 ± 176.94 μm3). The volumes of cardiomyocyte nuclei in the left ventricle were (53.42 ± 5.18 μm3) and in the right ventricle (52.85 ± 4.33 μm3). The volume of atrial nuclei (50.16 ± 4.57 μm3) is almost the same. Such ambiguous morphometric parameters of cardiomyocytes and their nuclei volumes are directly reflected in their nuclear-cytoplasmic ratio, which is the smallest in cardiomyocytes of the left ventricle (0.0136 ± 0.0062), larger in cardiomyocytes of the right ventricle (0.0219 ± 0.0079) and the largest (0.0430 ± 0.0096) in atrial cardiomyocytes. The obtained results of the study of the macro- and microscopic structure of the heart of domestic sheep significantly supplement the information on heart morphology in the relevant sections of comparative anatomy and histology and are a significant contribution to clinical cardiology.

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Dynamics of Ventricular Wall and Interventricular Septum Thickness of Rat’s Heart in the Early Postnatal Period in Normal Conditions and after Intranatal Injection of Dexamethasone

Abstract: Примітка: * показники статистично достовірні порівняно з контрольною групою.

Cited by 3 publications

References 3 publications

Peculiarities of organometry and morphoarchitectonics of the heart of the Domestic ram (Ovis aries L., 1758)

Peculiarities of organometry and morphoarchitectonics of the heart of the Domestic ram (Ovis aries L., 1758)

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Morphometric parameters of the heart of domestic sheep Ovis aries L., 1758

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