DYS459, DYS391, DYS388 and DYS19 genetic loci have high allelic frequency in patients with prostate cancer

Abstract

Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian prostate cancer patients and normal control males. The second objective was to evaluate the importance of these loci to develop prostate cancer. Blood samples were collected from 70 patients unrelated males living in middle and south of Iraq. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. Post PCR amplification was detected by using ABI Prism1 3130xl Genetic Analyzer 16-capillary array system, with POP-7™ Polymer and Data Collection Software, Genemapper version 3.5 software. A higher incidence of disease was found among males who had either allele 10 of DYS391 or allele 9 of DYS459. It is likely that Iraqi males who belong to Y-lineages with either allele 10 or allele 9 are more susceptible to develop prostate cancer, while those belonging to lineages with allele 17 of DYS456 and DYS19 are more resistant to the disease. This study shows the influence of genetic elements on prostate cancer, and it seems that DYS391 and DYS459 locus comprising with other loci have the potential to be used as a screening method for prediction of susceptibility to prostate cancer in Iraqi population.