Dysglycemia and Dyslipidemia Models in Nonhuman Primates: Part IV. Pancreatic Beta Cell Dysfunction and Diabetes Progression

Abstract

OPEN ACCESS Freely available online J ou rna l o f D ia be tes & M e ta bolism

Effects of mixed meal tolerance test on gastric emptying, glucose and lipid homeostasis in obese nonhuman primates

“…The higher baseline and net AUC of glucose in hyperglycemic group relative to normoglycemic group may indicate more glucose intolerance in the hyperglycemic group. This is consistent with a previous report in cynomolgus monkeys with naturally occurring diabetes 32 , 34 . Both insulin and C-peptide trended lower than basal levels in obese hyperglycemic than normoglycemic group at the 300 min blood sample collection, while glucose levels at 300 min were not different between the two groups.…”

“…In the current study the MMTT plus acetaminophen caused insignificant rise in blood glucose at 30 min measurement accompanied with significant increases in plasma insulin, C-peptide, aGLP-1, tGIP and TG after the meal administration. Baseline levels of overnight fasted glucose and insulin, time courses and AUCs of glucose and insulin for obese hyperglycemic and normoglycemic groups are consistent with reported data in cynomolgus monkey model of spontaneously developed diabetes 32 , 34 . It has been reported that fasted blood glucose > 125 mg/dL (> 7 mmol/L) represents progression from prediabetes to overt diabetes, and this could be hastened in NHPs by feeding the monkeys a high fat high glucose or high-sugar diets 44 .…”

“…The experiments were performed in male obese cynomolgus monkeys (Table 1 ) originally obtained from Jinjiekang monkey farm (Yuanjiang, Yunnan Province, China) and raised in our animal facility for 3 to 7 years (Taicang, Jiangsu Province, China). During the study, the enrolled monkeys were also grouped into those who had diabetes or not, based on our previous criteria 17 , 18 , 32 . Monkeys were individually housed and maintained in accordance with the guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC).…”