Dysregulated microRNA Expression in Serum of Non-Vaccinated Children with Varicella

Qi, Yuhua; Zhu, Zheng; Shi, Zhiyang; Ge, Yiyue; Zhao, Kun; Zhou, Minghao; Cui, Lunbiao

doi:10.3390/v6041823

Cited by 24 publications

(24 citation statements)

References 38 publications

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“…VZV infection is known to alter miRNA expression in the serum of herpes zoster patients [33,34]. We used small RNA-sequencing to detect miRNAs without the need for annotation and extended quantification over a wider dynamic range.…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

Lim

Song

et al. 2019

Pathogens

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Licensed live attenuated vaccines have been developed to prevent varicella zoster virus (VZV) infection, which causes chickenpox and shingles. The genomic sequences of both clinical- and vaccine-derived VZV strains have been analyzed previously. To further characterize the molecular signatures and complexity of wildtype (clinical) versus attenuated (vaccine-derived) VZV-mediated host cellular responses, we performed high-throughput next generation sequencing to quantify and compare the expression patterns of mRNAs and microRNAs (miRNAs) in primary human dermal fibroblasts (HDFs) infected with wildtype (YC01 low passage) and attenuated (YC01 high passage, SuduVax, and VarilRix) VZV strains. 3D-multidimensional scaling of the differentially expressed genes demonstrated the distinct grouping of wildtype and attenuated strains. In particular, we observed that HDFs infected with attenuated strains had more differentially expressed genes (DEGs) involved in the retinoic-acid inducible gene–I-like receptor and interferon-mediated signaling pathways compared with wildtype strains. Additionally, miRNA expression patterns were profiled following the infection of HDFs with VZV. Small RNA sequencing identified that several miRNAs were upregulated, including miR-146a-5p, which has been associated with other herpesvirus infections, whereas let-7a-3p was downregulated in both wildtype and attenuated VZV-infected cells. This study identified genes and miRNAs that may be essential in VZV pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

Lim

Song

et al. 2019

Pathogens

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“…The Database for Annotation, Visualization and Integrated Discovery (DAVID; Version 6.8) platform was used to analyze the functions of the identified target genes and the diverse signaling pathways in which they participate [16]. …”

Section: Methodsmentioning

confidence: 99%

Evaluation of microRNA Expression in Patients with Herpes Zoster

Huang

Zhang

et al. 2016

Viruses

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Reactivated varicella-zoster virus (VZV), which lies latent in the dorsal root ganglions and cranial nerves before its reactivation, is capable of causing herpes zoster (HZ), but the specific mechanism of virus reactivation and latency remains unknown. It was proposed that circulating microRNAs (miRNAs) in body fluids could potentially indicate infection. However, the connection between herpes zoster and circulating miRNAs has not been demonstrated. In this study, 41 HZ patients without superinfection were selected. The serum miRNA levels were analyzed by TaqMan low density array (TLDA) and confirmed individually by quantitative reverse transcription PCR (RT-qPCR) analysis. Thirty-five age-matched subjects without any infectious diseases or inflammation were selected as controls. The results showed that the serum miRNA expression profiles in 41 HZ patients were different from those of control subjects. Specifically, 18 miRNAs were up-regulated and 126 were down-regulated more than two-fold in HZ patients compared with controls. The subsequent confirmation of these results by qRT-PCR, as well as receiver operating characteristic (ROC) curve analysis, revealed that six kinds of miRNAs, including miR-190b, miR-571, miR-1276, miR-1303, miR-943, and miR-661, exhibited statistically significant enhanced expression levels (more than four-fold) in HZ patients, compared with those of healthy controls and herpes simplex virus (HSV) patients. Subsequently, it is proposed that these circulating miRNAs are capable of regulating numerous pathways and some may even participate in the inflammatory response or nervous system activity. This study has initially demonstrated that the serum miRNA expression profiles in HZ patients were different from those of uninfected individuals. Additionally, these findings also suggest that six of the altered miRNA could be potentially used as biomarkers to test for latent HZ infection.

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“…VZV encodes several miRNAs or small non-coding RNAs that regulate VZV infection in host cells [379]. Studies, using quantitative RT-PCR, have shown several circulating miRNAs in patients with VZV infection including: miR-197; miR-629; miR-363; miR-132; miR-122 as well as miR-1906; miR-571; miR1276; miR-1303; miR-943; and miR-661 [378,381]. Hence, circulating miRNAs can be potentially used as biomarkers of active or latent VZV infection [378,381].…”

Section: Sumo Proteins and Sumoylationmentioning

confidence: 99%

“…Studies, using quantitative RT-PCR, have shown several circulating miRNAs in patients with VZV infection including: miR-197; miR-629; miR-363; miR-132; miR-122 as well as miR-1906; miR-571; miR1276; miR-1303; miR-943; and miR-661 [378,381]. Hence, circulating miRNAs can be potentially used as biomarkers of active or latent VZV infection [378,381]. RNA sequencing analysis has shown that VZV infection has profound effect on differentiating keratinocytes thus promoting blistering and desquamation of the skin through upregulation of kallikreins and serine proteases and alteration of epidermal gene expression [382].…”

Section: Sumo Proteins and Sumoylationmentioning

confidence: 99%

The beneficial effects of varicella zoster virus

Al-Anazi¹,

Wk²,

Al-Jasser³

2019

J Hematol Clin Res

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Varicella zoster virus behaves differently from other herpes viruses as it differs from them in many aspects. Recently, there has been growing evidence on the benefi cial effects of the virus in immune compromised hosts and these effects are translated into prolongation of survival. The reported benefi cial effects of the virus include: (1) stimulation of bone marrow activity in patients with hematologic malignancies and bone marrow failure syndromes, (2) antitumor effects in various hematologic malignancies and solid tumors, and (3) association with graft versus host disease which has anticancer effects. Additionally, there are several reports on the safety of the live-attenuated even in severely immune suppressed individuals and on the emerging role of the virus in cancer immunotherapy. In this review, the following aspects of the virus will be thoroughly discussed: (1) new data on the genetic background, pathogenesis, vaccination, and new therapeutic modalities; (2) bone marrow microenvironment and hematopoiesis; (3) cells involved in the pathogenesis of the virus such as: mesenchymal stem cells, dendritic cells, natural killer cells, T-cells and mononuclear cells; (4) cellular proteins such as open reading frames, glycoproteins, promyelocytic leukemia protein, chaperons, and SUMOs; (5) extracellular vesicles, exosomes, and micro-RNAs; and (6) signaling pathways, cytokines, and interferons.

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Dysregulated microRNA Expression in Serum of Non-Vaccinated Children with Varicella

Cited by 24 publications

References 38 publications

Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

Evaluation of microRNA Expression in Patients with Herpes Zoster

The beneficial effects of varicella zoster virus

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