Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential

Abstract

Despite significant developments in its clinical treatment, the reported incidence and mortality of gastric cancer have exhibited marked increases. The molecular mechanisms of gastric cancer initiation and progression remain to be fully elucidated. The aim of the present study was to identify novel microRNAs (miRNAs/miRs) with a role in the peritoneal metastasis of gastric cancer by comparing the miRNA expression in the gastric cancer cell line GC9811 with that in its variant GC9811-P, a sub-cell line with a high potential for peritoneal metastasis. A miRNA microarray analysis identified 153 dysregulated miRNAs, including 74 upregulated and 79 downregulated miRNAs. Of these, four significantly upregulated miRNAs (miR-181a-5p, miR-106b-5p, miR-199a-3p and miR-148a-3p) and four downregulated miRNAs (miR-146a-5p, miR-21-5p, miR-222-3p and miR-221-3p) were selected and further confirmed by reverse transcription-quantitative polymerase chain reaction analysis. Furthermore, knockdown of miR-21-5p promoted the migration and invasion of GC9811 cells. Collectively, the results suggested that the miRNA expression profile in GC9811-P vs. GC9811 cells was altered to favor disease progression, and the dysregulated miRNAs, including miR-21-5p, may therefore provide novel biomarkers and potential therapeutic targets for gastric cancer metastasis.

Changes in microRNA expression associated with metastasis and survival in patients with uveal melanoma

“…Cluster-2 included 2 miRNAs, miR-221-3p and miR-222-3p, which were both downregulated in UM patients with metastasis. In a recent study, miR-221-3p and miR-222-3p were also downregulated in gastric cancer cells with high metastatic potential [18].…”

“…miRNAs typically regulate Research Paper gene expression by altering mRNA stability or repressing translation of mRNA to protein. Several miRNAs play an important role (tumor suppressors or tumor promoters) in cancer development and progression including metastasis [15][16][17][18][19][20]. However, data are limited regarding the role of miRNAs in metastatic UM [21][22][23].…”

<p>MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4</p>

“…1 It is reported that abnormal expression of miRNA is associated with tumorigenesis. [1][2][3] Some miRNA molecules can induce tumorigenesis and promote tumor progression as tumor promoters; on the other hand, some miRNA molecules act as tumor suppressors and inhibit the growth of tumor cells or induce their apoptosis. Therefore, miRNA molecules are considered as novel tumor markers and can be used in the diagnosis, treatment and prognosis prediction of tumors.…”

Pan‐cancer clinical and molecular analysis of racial disparities

“…All differentially expressed miRNAs are listed according to tumor type in Supporting Table 4. [24][25][26][27][28][29][30][31][32][33][34][35][36][37] Investigation of these miRNAs within published data indicates their role in oncogenesis. 2A) We performed pathway analysis to determine the clinical significance and biologic functions of the differentially expressed miRNAs for each cancer type.…”

“…AA miRNA expression data on TNBC were associated with an increase in the angiogenesis pathway, whereas miRNA expression data from AAs with non-TNBC tumor types were associated with a decrease in the angiogenesis pathway ( Table 2). In addition, all UCECs with dysregulated miRNA expression were of the EEA subtype; therefore, 25 Zhang 2017, 26 Sueta 2017 29 Shi 2017, 30 Meijer 2018 36 Duan 2016 37 Abbreviations: AA, African American; BRCA, breast cancer; KIRC, kidney renal clear cell carcinoma; miR, microRNA; NSCLC, non-small-cell lung cancer; PRAD, prostate adenocarcinoma; THCA, thyroid carcinoma; UCEC, uterine corpus endometrial carcinoma.…”