Dysregulated microRNAs involved in the progression of cervical neoplasm

Zeng, Kangkang; Zheng, Wenjing; Mo, Xiaoning; Liu, Fei; Li, Meiyi; Liu, Zhen; Zhang, Wei; Hu, Xiaoxia

doi:10.1007/s00404-015-3702-5

Cited by 40 publications

(44 citation statements)

References 36 publications

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“…miRNAs bind to the 3'-untranslated region of target mRNA sequences and subsequently repress their translation and expression (4). In addition, miRNAs are closely correlated with the development and progression of cervical cancer (5,6). They serve important roles in regulating the proliferation, apoptosis, invasion and metastasis of cervical cancer cells.…”

Section: Introductionmentioning

confidence: 99%

miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells

Cao

Meng

2017

Molecular Medicine Reports

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The present study aimed to investigate the role and the molecular mechanisms underlying the effects of microRNA-21 (miR-21) on the proliferation, apoptosis and colony formation of cervical cancer cells, and to examine the role of miR-21 in mediating the sensitivity of cervical cancer cells to paclitaxel (PTX). Reverse transcription‑quantitative polymerase chain reaction was employed to determine the level of miR‑21 in various cervical cancer and normal cervical cells. The results revealed that the expression levels of miR-21 in cervical cancer cells were markedly higher when compared with normal cervical cells. Subsequently, a miR‑21 inhibitor or negative control (NC) was transfected into cervical cancer cells. Cell viability, colony formation and apoptosis were then analyzed using an MTT assay, crystal violet and Annexin V-fluorescein isothiocyanate/propidium iodide staining, respectively. The protein expression level of B-cell lymphoma‑2 (Bcl‑2), Bcl‑2‑associated X (Bax), programmed cell death 4 (PDCD4), survivin, c‑myc, phosphatase and tensin homolog (PTEN) and phosphorylated (p)‑AKT were determined by western blot analysis. The sensitivity of cervical cancer cells to PTX (25, 50 and 100 µg/ml) was characterized using an MTT assay. The results demonstrated that the miR-21 inhibitor promoted apoptosis of cervical cancer cells and suppressed their proliferation and colony formation when compared with the NC. In addition, the expression levels of Bcl‑2, survivin, c‑myc and p‑AKT were significantly downregulated in cells transfected with the miR‑21 inhibitor, whilst the expression levels of Bax, PDCD4 and PTEN were significantly upregulated. Furthermore, the miR‑21 inhibitor significantly enhanced the inhibition efficacy of PTX at a range of concentrations in cervical cancer cells. It was concluded that inhibition of miR‑21 suppressed cell proliferation and colony formation through regulating the PTEN/AKT pathway, and improved PTX sensitivity in cervical cancer cells. The results of the present study may contribute to the development of miRNA‑based cervical cancer therapy in the future.

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Section: Introductionmentioning

confidence: 99%

miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells

Cao

Meng

2017

Molecular Medicine Reports

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“…The initiation and progression of cervical cancer is a multi-step process, involving multiple factors and the transformation of normal cervical epithelium into cervical intraepithelial neoplasia, which subsequently transforms into invasive cervical cancer (2,3). A number of studies have demonstrated that persistent infection with high-risk human papillomavirus (HPV) serves an important role in the initiation and progression of cervical cancer (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-10b inhibits proliferation, migration and invasion in cervical cancer cells via direct targeting of insulin-like growth factor-1 receptor

2017

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Abstract. MicroRNAs are deregulated in numerous types of human cancers and have crucial roles in the carcinogenesis and progression of human cancers. MicroRNA-10b (miR-10b) has been studied in several types of human cancer. However, the expression and roles of miR-10b in cervical cancer remain unknown. In the present study, the expression, functions and molecular mechanisms of miR-10b were explored in cervical cancer. The present data revealed that miR-10b was significantly downregulated in cervical cancer tissues and cell lines. In addition, miR-10b overexpression inhibited the proliferation, migration and invasion of cervical cancer cells, while miR-10b under-expression had the opposite effect. Based on bioinformatics analysis, a luciferase reporter assay and western blot analysis, insulin-like growth factor-1 receptor (IGF-1R) was identified as a direct target of miR-10b in cervical cancer. In addition, IGF-1R small interfering RNA-mediated knockdown of IGF-1R also inhibited the proliferation, migration and invasion of the cervical cancer cells. In conclusion, the present study demonstrated that miR-10b serves an important role in cervical cancer progression by targeting IGF-1R.

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“…12Y14 However, the role of miRNAs in cervical cancer development and precise mechanism of its actions remains largely elusive. Several recent microarray analyses showed that miR-195 expression was significantly lower in cervical cancer tissues relative to normal tissues, 15,16 and a large body of studies has found that miR-195 regulates tumor progression in a variety of cancers as a tumor-suppressive miRNA. 17Y19 However, the potential involvement of miR-195 in cervical cancer remains much to be determined.…”

mentioning

confidence: 98%

MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3

Zhou¹,

Han²,

Zhou³

et al. 2016

International Journal of Gynecological Cancer

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Dysregulated microRNAs involved in the progression of cervical neoplasm

Abstract: Our study demonstrated that miRNAs are aberrantly expressed in cervical cancer and cervical preneoplastic lesions. These miRNAs may be involved in the progression of cervical neoplasm as potential tumor suppressor genes or oncogenes.

Cited by 40 publications

References 36 publications

miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells

miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells

MicroRNA-10b inhibits proliferation, migration and invasion in cervical cancer cells via direct targeting of insulin-like growth factor-1 receptor

MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3

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