E. coli Bacteremia Strains - High diversity and Associations with Agerelated Clinical Phenomena

TB, Melby KK Wyller

doi:10.4172/2327-5073.1000140

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…As markers of genetic relationships, we included 212 community-acquired E. coli bacteremia isolates, 38 other human strains from the E. coli Reference (ECOR)-collection obtained from the Microbial Evolutionary Laboratory (State University of Michigan, USA), four Enterohemorrhagic E. coli (EHEC) strains associated with hemorrhagic uremic syndrome (HUS) from the strain collection at the Norwegian Institute of Public Health, as described (Wester et al, 2013, 2014). The community-acquired E. coli isolates causing blood stream infection (BSI) were classified as non-severe, early organ failure (≥organs affected within 1 day of admittance to hospital), or in-hospital death within 14 days of admission (Wester et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Grevskott¹,

Svanevik²,

Sunde

et al. 2017

Front. Microbiol.

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The mechanisms for the development and spread of antibacterial resistance (ABR) in bacteria residing in environmental compartments, including the marine environment, are far from understood. The objective of this study was to examine the ABR rates in Escherichia coli and other Enterobacteriaceae isolates obtained from marine bivalve mollusks collected along the Norwegian coast during a period from October 2014 to November 2015. A total of 549 bivalve samples were examined by a five times three tube most probable number method for enumeration of E. coli in bivalves resulting in 199 isolates from the positive samples. These isolates were identified by biochemical reactions and matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, showing that 90% were E. coli, while the remaining were species within the genera Klebsiella, Citrobacter, and Enterobacter. All 199 isolates recovered were susceptibility tested following the European Committee on Antimicrobial Susceptibility Testing disk diffusion method. In total, 75 of 199 (38%) isolates showed resistance to at least one antibacterial agent, while multidrug-resistance were seen in 9 (5%) isolates. One isolate conferred resistance toward 15 antibacterial agents. Among the 75 resistant isolates, resistance toward extended-spectrum penicillins (83%), aminoglycosides (16%), trimethoprim (13%), sulfonamides (11%), tetracyclines (8%), third-generation cephalosporins (7%), amphenicols (5%), nitrofurans (5%), and quinolones (5%), were observed. Whole-genome sequencing on a selection of 10 E. coli isolates identified the genes responsible for resistance, including blaCTX-M genes. To indicate the potential for horizontal gene transfer, conjugation experiments were performed on the same selected isolates. Conjugative transfer of resistance was observed for six of the 10 E. coli isolates. In order to compare E. coli isolates from bivalves with clinical strains, multiple-locus variable number tandem repeats analysis (MLVA) was applied on a selection of 30 resistant E. coli isolates. The MLVA-profiles were associated with community-acquired E. coli strains causing bacteremia. Our study indicates that bivalves represent an important tool for monitoring antibacterial resistant E. coli and other members of the Enterobacteriaceae family in the coastal environment.

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Section: Methodsmentioning

confidence: 99%

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Grevskott¹,

Svanevik²,

Sunde

et al. 2017

Front. Microbiol.

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“…Escherichia coli is gram-negative, rod-shaped bacteria that inhabit the intestine of warm blood animals. Its presence in meat sample refer to fecal contamination, and some of its strains can cause many diseases and diarrhea (Lubote et al 2014;Wester et al 2014). Enterobacteriaceae members are the most challenging and prevalent bacterial contaminants in raw and processed meat worldwide.…”

Section: Morphological and Molecular Identification Bacterial Isolatesmentioning

confidence: 99%

Short Communication: Molecular identification and assessment of bacterial contamination of frozen local and imported meat and chicken in Basrah, Iraq using 16S rDNA gene

MOHAMMAD

ALYOUSIF²

2022

Biodiversitas

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Abstract. Mohammad AJ, Alyousif NA. 2022. Short Communication: Molecular identification and assessment of bacterial contamination of frozen local and imported meat and chicken in Basrah, Iraq using 16S rDNA gene. Biodiversitas 23: 1598-1604. Meat is the main food source of daily meals with highly beneficial effects on human health. It is susceptible to the growth and reproduction of various bacteria due to its composition, which leads to its spoilage, and being an important carrier of pathogenic bacteria. The study aimed to isolate and identify the bacteria contamination of frozen local and imported meat and chicken samples using 16S rDNA gene. Twenty meat samples were collected randomly from local and imported meat sold in markets of Basrah province, Iraq. The results showed that the total aerobic bacteria count was within acceptable limits in all samples. Fifty-seven bacterial isolates were isolated and identified by amplification and sequencing of 16S rDNA gene, where the gram-positive bacteria were the most abundant 34(59.6%), and S. pasteuri is the most common species in the samples with 14 (24%) bacterial isolates out of the total isolates. Fifteen isolates were identified as new strains and their sequences were deposited at GenBank (NCBI) under different accession numbers (MZ964582-MZ964596). The meat and chicken have many bacteria on their surface, therefore the products must be frozen, and sanitary precautions should be taken into consideration.

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Individuality, phenotypic differentiation, dormancy and ‘persistence’ in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology

2015

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For bacteria, replication mainly involves growth by binary fission. However, in a very great many natural environments there are examples of phenotypically dormant, non-growing cells that do not replicate immediately and that are phenotypically 'nonculturable' on media that normally admit their growth. They thereby evade detection by conventional culture-based methods. Such dormant cells may also be observed in laboratory cultures and in clinical microbiology. They are usually more tolerant to stresses such as antibiotics, and in clinical microbiology they are typically referred to as 'persisters'. Bacterial cultures necessarily share a great deal of relatedness, and inclusive fitness theory implies that there are conceptual evolutionary advantages in trading a variation in growth rate against its mean, equivalent to hedging one's bets. There is much evidence that bacteria exploit this strategy widely. We here bring together data that show the commonality of these phenomena across environmental, laboratory and clinical microbiology. Considerable evidence, using methods similar to those common in environmental microbiology, now suggests that many supposedly non-communicable, chronic and inflammatory diseases are exacerbated (if not indeed largely caused) by the presence of dormant or persistent bacteria (the ability of whose components to cause inflammation is well known). This dormancy (and resuscitation therefrom) often reflects the extent of the availability of free iron. Together, these phenomena can provide a ready explanation for the continuing inflammation common to such chronic diseases and its correlation with iron dysregulation. This implies that measures designed to assess and to inhibit or remove such organisms (or their access to iron) might be of much therapeutic benefit.

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E. coli Bacteremia Strains - High diversity and Associations with Agerelated Clinical Phenomena

Cited by 4 publications

References 42 publications

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Short Communication: Molecular identification and assessment of bacterial contamination of frozen local and imported meat and chicken in Basrah, Iraq using 16S rDNA gene

Individuality, phenotypic differentiation, dormancy and ‘persistence’ in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology

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