E. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens

Abstract

Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NdV). developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (hN) protein can protect the poultry industry. in the present study, the full-length hN protein was expressed in Escherichia coli (E. coli) Bl21 (dE3) cells, purified via affinity chromatography and detected via western blot analysis using his-specific antibodies. the purified hN protein was further evaluated in chickens to study the immune response against NdV. the successful production of hN-specific igY proved the activity of the purified hN protein. igY was present in the serum of immunized chickens. however, the immune response was higher in chickens immunized with purified hN protein along with complete and incomplete adjuvants than in chickens immunized with only the hN protein.

A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice

“…The expression level of Osr2HN in rice reached 3.7 mg/g. Although a simple comparison is not accurate, the amount of protein production is compared as mL of liquid culture equivalent to 1 g, In E. coli expression system, yields of HN were 0.034 to 0.37 mg/mL ( 48 – 50 ), with production based on the manufacturer’s instruction. This expression level is lower than Osr2HN in rice.…”