ÊA simple approach for THE DETERMINATION AND CHARACTERIZATION OF TERNARY PHASE DIAGRAMS of aqueous two-phase systems composed of water, poly(ethylene) glycol and sodium carbonate

Capela, Emanuel V.; Santos, João H. P. M.; Boal-Palheiros, Isabel; Carvalho, Pedro J.; Coutinho, J.; Ventura, Sónia P. M.; Freire, Mara G.

doi:10.18260/2-1-370.660-108013

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…The procedure was repeated after diluting the earlier cloud point into the homogenous monophasic solution by dropwise addition of water until the extract volume gets minimal. The mass fractions of SDES and salt observed in each cloud point were plotted individually on the X and Y axes correspondingly to obtain the binodal curve of SDES as described by Capela et al 27 .…”

Section: Methodsmentioning

confidence: 99%

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Muniasamy

Balamurugan

Rajamahendran

et al. 2022

Sci Rep

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Fibrinolytic protease (FLP) is a therapeutic enzyme used in the treatment of thrombolytic diseases. The present study proposed the concept of pH-driven swappable micellar two-phase extraction for the concurrent production and purification of FLP from Bacillus subtilis at cloud point extraction. Extractive fermentation was carried out with a pH swap mechanism and FLP was extracted to the top phase by surfactant deep eutectic solvents (SDES). Shrimp waste was chosen as a sustainable low-cost substrate that yielded a maximum protease of 185 U/mg. Six SDESs were synthesized with nonionic surfactants as hydrogen bond donors and quaternary ammonium salts as hydrogen bond acceptors and their association was confirmed by H1 NMR. Thermophysical investigation of the synthetic SDES was accomplished as a function of temperature. Response surface methodology for extractive fermentation was performed with the concentration of SADES (35% w/v), Na2SO4 (15% w/v) and pH (6.3) as variables and the enzyme activity (248 IU/mg) as a response. Furthermore, purification using gel filtration chromatography was used to quantify the amount of enzyme obtained in the extraction phase (849 IU/ml). After final purification with an anion exchange column, the maximum purity fold (22.32) with enzyme activity (1172 IU/ml) was achieved. The in-vitro fibrinolytic activity has been confirmed using a fibrin plate assay.

show abstract

Section: Methodsmentioning

confidence: 99%

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Muniasamy

Balamurugan

Rajamahendran

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The procedure was repeated after diluting the earlier cloud point to the homogenous monophasic solution by dropwise addition of water until the extract volume was minimal. The mass fractions of SDES and salt observed at each cloud point were plotted individually on the X-and Y-axes to obtain the binodal curve of SDES as described by capela et al (49).…”

Section: Binodal Curve Determination Of Sdesmentioning

confidence: 99%

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Muniasamy

Rathnasamy

2021

Preprint

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Fibrinolytic protease (FLP) is a therapeutic enzyme used in the treatment of thrombolytic diseases. The present study proposed the concept of pH-driven swappable micellar two-phase extraction for the concurrent production and purification of FLP from Bacillus subtilis at cloud point extraction. Extractive fermentation was carried out with a pH swap mechanism, and FLP was extracted to the top phase by surfactant deep eutectic solvents (SADES). Shrimp waste was chosen as a sustainable low-cost substrate that yielded a maximum protease of 185 U/mg. Six SDESs were synthesized with nonionic surfactants as hydrogen bond donors and quaternary ammonium salts as hydrogen bond acceptors, and their association was confirmed by H1 NMR. Thermophysical investigation of the synthetic SADES was accomplished as a function of temperature. Response surface methodology for extractive fermentation was performed with the concentration of SADES (35% w/v), Na2SO4 (15% w/v) and pH (6.3) as variables and the enzyme activity (248 IU/mg) as a response. Furthermore, purification using gel filtration chromatography was used to quantify the amount of enzyme obtained in the extraction phase (849 IU/ml). After final purification with an anion exchange column, the maximum purity fold (22.32) with enzyme activity (1172 IU/ml) was achieved. The in vitro fibrinolytic activity was confirmed using a fibrin plate assay.

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Widening the Stimuli‐Responsiveness of Aqueous Biphasic Systems by Changing the Ionic Liquid Cation Basicity

Rufino,

Capela,

Quental

et al. 2024

Chemistry A European J

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Herein we demonstrate the formation of new stimuli‐responsive aqueous biphasic systems (ABS), able to respond simultaneously to temperature and pH, or just to one stimulus, therefore allowing the design of more sustainable separation processes. This dual behavior is achieved with ABS formed by mono or dicationic protic ionic liquids as phase‐forming components, being defined by the ionic liquid cation chemical structure or its basicity. While ABS comprising monocationic ionic liquids only respond to the effect of temperature, systems comprising dicationic ionic liquids are simultaneously affected by both temperature and pH variations. Dicationic ionic liquids are here identified as the key to unlock a double response to stimuli, which is due to the presence of two pKa values afforded by the cation. The reported findings contribute to increase the customizability of double stimuli‐responsive ABS based on ionic liquids, whose development was up to date limited to ionic liquids bearing pH‐responsive anions, opening the door towards the development of more sustainable separation processes.

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ÊA simple approach for THE DETERMINATION AND CHARACTERIZATION OF TERNARY PHASE DIAGRAMS of aqueous two-phase systems composed of water, poly(ethylene) glycol and sodium carbonate

Abstract: He received his Biochemistry M.S. degree in 2016 from the University of Aveiro, Portugal. His research interests cover the development of new platforms for the purification of biopharmaceuticals using ionic liquids.

Cited by 6 publications

References 26 publications

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Switchable deep eutectic solvent driven micellar extractive fermentation of ultrapure fibrin digesting enzyme from Bacillus subtilis

Widening the Stimuli‐Responsiveness of Aqueous Biphasic Systems by Changing the Ionic Liquid Cation Basicity

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