Early apoptosis is associated with improved developmental potential in bovine oocytes

Li, H.J.; Liu, D.J.; Cang, Ming; Wang, L.M.; Jin, Minghui; Ma, Yanmin; Bou, Shorgan

doi:10.1016/j.anireprosci.2008.09.018

Cited by 45 publications

(59 citation statements)

References 41 publications

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“…In this regard, [45]. While these authors believe that quantifying the expression of apoptotic genes in general has little significance for apoptosis detection, in accordance to our finding, Li et al (2008) in their study concluded that expression abundance of the Bax and Bcl-2 genes are good markers for oocyte apoptosis and subsequent embryo development [46]. At the blastocyst stage, results of this study also showed that Bcl-2 mRNA was increased in BCB + blastocysts compared with the control and BCB − blastocysts, and expression of this gene was decreased in BCB − blastocysts in compression with control blastocysts.…”

Section: Discussionsupporting

confidence: 77%

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia

Mohammadi‐Sangcheshmeh

Dehghani-Mohammadabadi

et al. 2013

J Assist Reprod Genet

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Purpose To associate glucose-6-phosphate dehydrogenase (G6PDH) activity in goat oocytes with intracellular glutathione (GSH) content, meiotic competence, developmental potential, and relative abundance of Bax and Bcl-2 genes transcripts. Methods Goat oocytes were exposed to brilliant cresyl blue (BCB) staining test and categorized into BCB + (bluecytoplasm), and BCB − (colorless-cytoplasm) groups. A group of oocytes were not exposed to BCB test and was considered as a control group. After maturation in vitro, a group of oocytes were used for determination of nuclear status and intracellular GSH content while another group was subjected to parthenogenetic activation followed by in vitro embryo culture.Results We found that BCB + oocytes not only yielded higher rate of maturation, but also showed an increased level of intracellular GSH content than BCB − and control oocytes.Furthermore, BCB + oocytes produced more blastocysts than BCB − and control oocytes. Our data revealed that the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes were interacted with G6PDH-activity in mature oocyte, their surrounding cumulus cells, and blastocyst-stage embryos.Conclusions The results of this study demonstrate that selection of goat oocytes based on G6PDH-activity through the BCB test improves their developmental competence, increases intracellular GSH content, and affects the expression of the apoptosis-related genes.

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Section: Discussionsupporting

confidence: 77%

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Abazari-Kia

Mohammadi‐Sangcheshmeh

Dehghani-Mohammadabadi

et al. 2013

J Assist Reprod Genet

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“…3) are linked to apoptosis and/ or oxidative stress, which is associated with insufficient oxygen supply leading to atresia. Even if early atresia has been associated with developmental competence (Blondin & Sirard 1995, Li et al 2009), it has a negative effect if it is a long-lasting process.…”

Section: Gene Analysismentioning

confidence: 99%

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Nivet¹,

Vigneault²,

Blondin³

et al. 2013

Reproduction

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One of the challenges in mammalian reproduction is to understand the basic physiology of oocyte quality. It is believed that the follicle status is linked to developmental competence of the enclosed oocyte. To explore the link between follicles and competence in cows, previous research at our laboratory has developed an ovarian stimulation protocol that increases and then decreases oocyte quality according to the timing of oocyte recovery post-FSH withdrawal (coasting). Using this protocol, we have obtained the granulosa cells associated with oocytes of different qualities at selected times of coasting. Transcriptome analysis was done with Embryogene microarray slides and validation was performed by real-time PCR. Results show that the major changes in gene expression occurred from 20 to 44 h of coasting, when oocyte quality increases. Secondly, among upregulated genes (20-44 h), 25% were extracellular molecules, highlighting potential granulosa signaling cascades. Principal component analysis identified two patterns: one resembling the competence profile and another associated with follicle growth and atresia. Additionally, three major functional changes were identified: i) the end of follicle growth (BMPR1B, IGF2, and RELN), involving interactions with the extracellular matrix (TFPI2); angiogenesis (NRP1), including early hypoxia, and potentially oxidative stress (GFPT2, TF, and VNN1) and ii) apoptosis (KCNJ8) followed by iii) inflammation (ANKRD1). This unique window of analysis indicates a progressive hypoxia during coasting mixed with an increase in apoptosis and inflammation. Potential signaling pathways leading to competence have been identified and will require downstream testing. This preliminary analysis supports the potential role of the follicular differentiation in oocyte quality both during competence increase and decrease phases.Reproduction (2013) 145 555-565

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“…The mRNA content in oocytes is affected by animal nutrition (Pisani et al 2008), follicle diameter (Caixeta et al 2009), IVM culture media (Salhab et al 2011), in vivo and in vitro conditions (Wells & Patrizio 2008) and apoptosis (Li et al 2009). Thus, mRNA stored in oocytes could represent a valuable tool as a molecular marker for oocyte quality.…”

Section: Introductionmentioning

confidence: 99%

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

Catalá¹,

Izquierdo²,

Uzbekova³

et al. 2011

Reproduction

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The aim of this study was to test the Brilliant Cresyl Blue (BCB) stain to select prepubertal sheep oocytes for in vitro blastocyst production. Oocyte diameter, mitochondrial activity, maturation-promoting factor (MPF) activity and mRNA relative expression (RE) of genes related to metabolism (ATPase Na C /K C transporting a 1 (ATP1A1) and cytochrome c oxidase subunit 1 (COX1)) and constitutive function of the cell (cytoplasmic polyadenylation-element-binding protein (CPEB) and S100A10) were assessed. Immature oocytes were exposed to different BCB concentrations (13, 26, 39 and 52 mM) and classified according to their cytoplasm colouration as grown BCBC (blue cytoplasm) and growing BCBK (colourless cytoplasm). Staining oocytes with 13 mM BCB during 60 min allows selection of (BCBC) the largest (123.66 mm) and most competent oocytes to develop to the blastocyst stage (21%) with a higher number of cells (69.71G6.19S.E.M.) compared with non-stained BCBK oocytes (106.82 mm, 9% and 45.91G3.35 S.E.M. respectively). Mitochondrial activity, assessed by MitoTracker Orange CMTMRos probe, was significantly higher in BCBC than in BCBK oocytes after in vitro maturation (3369 and 1565 AU respectively). MPF activity was assessed by CDC2 kinase activity assay showing significantly higher activity at metaphase II stage in BCBC than in BCBK oocytes (1.479G0.09 and 1.184G0.05 optical density respectively). The genes analysed in this work, ATP1A1, COX1, CPEB and S100A10, did not show significant effect in mRNA RE between BCB selected oocytes. In conclusion, BCB stains larger and more competent oocytes to develop to the blastocyst stage with more active mitochondria and MPF activity and higher blastocyst cell number.

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Early apoptosis is associated with improved developmental potential in bovine oocytes

Cited by 45 publications

References 41 publications

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Brilliant Cresyl Blue stain selects largest oocytes with highest mitochondrial activity, maturation-promoting factor activity and embryo developmental competence in prepubertal sheep

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