Early candidate biomarkers found from urine of glioblastoma multiforme rat before changes in MRI

Ni, Yanying; Zhang, Fanshuang; An, Manxia; Yin, Wei; Gao, Youhe

doi:10.1007/s11427-017-9201-0

Cited by 50 publications

(47 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glutamate is the major excitatory neurotransmitter in the central nervous system, and abnormally increased glutamate levels destroy normal tissues . These results suggest that this effect may be due to differences in the invasion abilities and patterns of W256 and C6 cells, as C6 cells diffusely infiltrated the brain parenchyma and displayed strongly invasive extension …”

Section: Resultsmentioning

confidence: 96%

“…The subcutaneous W256 model and the intracerebral C6 model were used in the Wu et al and Ni et al studies, respectively. The right flanks of male Wistar rats (n = 10) were subcutaneously injected with 2 × 10 6 viable W256 cells in 200 μL of phosphate‐buffered saline (PBS), and urine samples were collected on days 0, 4, 6, 9, and 14 after injection.…”

Section: Methodsmentioning

confidence: 99%

“…Data from the Wu et al and Ni et al studies were used in this study for comparison of the different tumor models. In the intracerebral C6 model, the raw data collected by LC‐MS/MS on days 2, 6 10, and 13 from three intracerebral C6 rats and on day 2 from three control rats were reanalyzed using Scaffold for better and consistent comparison.…”

Section: Methodsmentioning

confidence: 99%

“…During collection, rats were individually placed in metabolic cages overnight naturally for 10 hours to collect urine samples, and no water or food was provided to avoid urine contamination. The subcutaneous W256 model and the intracerebral C6 model were used in the Wu et al 10 and Ni et al 11 studies, respectively. The right flanks of male Wistar rats (n = 10) were subcutaneously injected with 2 × 10 6 viable W256 cells in 200 μL of phosphate-buffered saline (PBS), and urine samples were collected on days 0, 4, 6, 9, and 14 after injection.…”

Section: Animal Modelsmentioning

confidence: 99%

“…We recently demonstrated that urinary proteins could enable the early detection of cancer in a Walker 256 (W256) tumor‐bearing model using a urinary proteomics approach . In a glioma rat model injected with C6 cells, changes in urinary proteins were found earlier than magnetic resonance imaging (MRI) changes . Therefore, we asked whether inoculating the same tumor cells into different tissues would cause different urinary changes and whether different tumor cells inoculated in the same organ would cause different urinary proteome changes.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Zhang

Meng

et al. 2019

Cancer Medicine

Self Cite

View full text Add to dashboard Cite

Background Patients with primary and metastatic brain cancer have an extremely poor prognosis, mostly due to the late diagnosis of disease. Urine, which lacks homeostatic mechanisms, is an ideal biomarker source that accumulates early and highly sensitive changes to provide information about the early stage of disease. Methods A rat model mimicking the local tumor growth process in the brain was established with intracerebral Walker 256 (W256) cell injection. Urine samples were collected on days 3, 5, and 8 after injection, and then analyzed by liquid chromatography coupled with tandem mass spectrometry. Results In the intracerebral W256 model, no obvious clinical manifestations or abnormal magnetic resonance imaging (MRI) signals were found on days 3 or 5; at these time points, 9 proteins were changed significantly in the urine of all eight tumor rats. On day 8, when tumors were detected by MRI, 25 differential proteins were identified, including 10 that have been reported to be closely related to brain metastasis or primary tumors. The differential urinary proteome was compared with those from the subcutaneous W256 model and the intracerebral C6 model. Few differential proteins overlapped, and specific differential protein patterns were observed among the three models. Conclusions These findings demonstrate that early changes in the urine proteome can be detected in the intracerebral W256 model. The urinary proteome can reflect the difference when tumor cells with different growth characteristics are inoculated into the brain and when identical tumor cells are inoculated into different areas, specifically, the subcutis and the brain.

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Animal Modelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations