Early Career Scientists’ Guide to the Red Blood Cell – Don’t Panic!

Bogdanova, Anna; Kaestner, Lars

doi:10.3389/fphys.2020.00588

Cited by 9 publications

(8 citation statements)

References 54 publications

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“…More importantly, our synthesis proved that erythrocyte metabolism is more than just an “internal” biochemical matter, but a matter of controlling one of the most important biological processes: loading, transportation, and delivery of oxygen. Erythrocyte biology research is at a crucial juncture, 27 where physiology approaches, computational modelling methods, and sophisticated analytical tools are being implemented. We propose that characterizing the dynamics of the erythrocyte network (e.g., the biochemical pathways activated by stimuli such as oxidative stress) could reveal important insights for personalized and/or transfusion medicine.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, in fields like environmental and exercise physiology, which focus heavily on oxygen, improvements in oxygen transport and release have been attributed exclusively to increased erythrocyte count or haemoglobin concentration. In the same vein, erythrocyte experts [21][22][23][24][25][26][27][28] have repeatedly emphasized that older literature tended to oversimplify the erythrocyte as an inert cell. As our understanding of the complexity of erythrocyte metabolism expands, these views have become obsolete.…”

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confidence: 99%

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Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

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Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map. The focus of this map is on the main biochemical pathways consisting of glycolysis, the pentose phosphate pathway, redox metabolism, oxygen metabolism, purine/nucleoside metabolism, and membrane transport. Other recently emerging pathways are also curated, like the methionine salvage pathway, the glyoxalase system, carnitine metabolism, and the lands cycle, as well as remnants of the carboxylic acid metabolism. An additional goal of this review is to present the dynamics of erythrocyte metabolism, providing key numbers used to perform basic quantitative analyses. By synthesizing experimental and computational data, we conclude that glycolysis, pentose phosphate pathway, and redox metabolism are the foundations of erythrocyte metabolism. Additionally, the erythrocyte can sense oxygen levels and oxidative stress adjusting its mechanics, metabolism, and function. In conclusion, fine‐tuning of erythrocyte metabolism controls one of the most important biological processes, that is, oxygen loading, transport, and delivery.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

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“…While the response of U937 cells to hypertonic shrinkage or hypotonic swelling is very rapid (minutes), for erythrocytes these responses can be much slower. There are indications that human erythrocytes are among the very few cell types that lack volume regulatory mechanisms such as RVI or RVD [ 24 , 33 , 34 ]. Experimental studies of the response of the human erythrocytes to hypotonic swelling have been contradictory [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Erythrocytes have recently been shown to cause vascular dysfunction in COVID-19 [ 32 ]. Despite years of research, RBCs remain “a mysterious and fascinating study objects” [ 33 ]. Recently, mainly as a result of researches focused on the disease, there has been a significant increase in the amount of data on the presence and activity of signaling pathways that regulate the function of erythrocytes [ 34 – 37 ].…”

Section: Introductionmentioning

confidence: 99%

Unidirectional fluxes of monovalent ions in human erythrocytes compared with lymphoid U937 cells: Transient processes after stopping the sodium pump and in response to osmotic challenge

et al. 2023

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Recently, we have developed software that allows, using a minimum of required experimental data, to find the characteristics of ion homeostasis and a list of all unidirectional fluxes of monovalent ions through the main pathways in the cell membrane both in a balanced state and during the transient processes. Our approach has been successfully validated in human proliferating lymphoid U937 cells during transient processes after stopping the Na/K pump by ouabain and for staurosporine-induced apoptosis. In present study, we used this approach to find the characteristics of ion homeostasis and the monovalent ion fluxes through the cell membrane of human erythrocytes in a resting state and during the transient processes after stopping the Na/K pump with ouabain and in response to osmotic challenge. Due to their physiological significance, erythrocytes remain the object of numerous studies, both experimental and computational methods. Calculations showed that, under physiological conditions, the K+ fluxes through electrodiffusion channels in the entire erythrocyte ion balance is small compared to the fluxes through the Na/K pump and cation–chloride cotransporters. The proposed computer program well predicts the dynamics of the erythrocyte ion balance disorders after stopping the Na/K pump with ouabain. In full accordance with predictions, transient processes in human erythrocytes are much slower than in proliferating cells such as lymphoid U937 cells. Comparison of real changes in the distribution of monovalent ions under osmotic challenge with the calculated ones indicates a change in the parameters of the ion transport pathways through the plasma membrane of erythrocytes in this case. The proposed approach may be useful in studying the mechanisms of various erythrocyte dysfunctions.

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“…It has become increasingly apparent that red cells are not simply quiescent carrier vehicles for haemoglobin (Hb) but rather have the ability to contribute to a rich complexity of responses. Physiologically, they participate in the overall homeostasis of organisms (eg from this journal: Pretini et al, 2019 ; Bogadnova and Kaestner, 2020 ), whilst, pathologically, their abnormal behaviour may contribute to a number of disease states (eg from elsewhere: Gallagher, 2017 ; Bernard, 2021 ). In humans, much evidence pertaining to the diversity of red cell function has been recently derived from a better appreciation of red cell phenotype reflects genotype in both healthy individuals and in patients with various disease states.…”

Section: Introductionmentioning

confidence: 99%

Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?

Hannemann

Gibson

2022

Front. Physiol.

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Red cells from LK sheep represent an important paradigm for control of KCl cotransport activity, as well as being important to sheep erythroid function. A previous report (Godart et al., 1997) suggested that autologous plasma markedly inhibits red cell KCC activity and identified the presence of the bicarbonate/CO2 buffer system as the probable cause. Findings were restricted, however, to red cells from patients with sickle cell disease (SCD) swollen anisotonically and carried out at a very high O2 tension (c.700 mmHg). It was therefore important to investigate the generality of the effect described and whether it was also relevant to the two main stimuli for KCC activity encountered most often by circulating red cells in vivo - low pH in active muscle beds during exercise and high urea concentrations in the renal medulla during antidiuresis. Results confirm that inhibition was significant in response to anisotonic swelling with KCC activity in MOPS-buffered saline (MBS) vs. bicarbonate-buffered saline (BBS) and in MBS vs. plasma both reduced (by about 25 and 50%, respectively). By contrast, however, inhibition was absent at low pH and in high concentrations of urea. These findings suggest therefore that red cell KCC activity represents an important membrane permeability in vivo in red cells suspended in plasma. They are relevant, in particular, to sheep red cells, and may also be important by extension to those of other species and to the abnormal red cells found in human patients with SCD.

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Early Career Scientists’ Guide to the Red Blood Cell – Don’t Panic!

Cited by 9 publications

References 54 publications

Erythrocyte metabolism

Erythrocyte metabolism

Unidirectional fluxes of monovalent ions in human erythrocytes compared with lymphoid U937 cells: Transient processes after stopping the sodium pump and in response to osmotic challenge

Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?

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