Early cleavage and the maternal zygotic transition in bovine embryos

Barnes, Frank L.; Eyestone, W.H.

doi:10.1016/0093-691x(90)90605-s

Cited by 161 publications

(124 citation statements)

References 42 publications

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“…Few reports have been published on cleavage kinetics of bovine embryos produced in vitro (Sirard and Lambert, 1985; First and Barnes, 1989;Pollard et ah, 1991;Barnes (Massip and Mulnard, 1980;Massip et ah, 1982Massip et ah, , 1983a.…”

Section: Introductionmentioning

confidence: 99%

Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Grisart¹,

Massip²,

Dessy³

1994

Reproduction

124

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Development of bovine embryos produced in vitro from the one-cell to the blastocyst stage in serum-free oviduct-conditioned medium was five\p=n-\eight-cell), the higher the probability that they developed into morula\p=n-\blastocyst: 70% of the embryos reaching the two-cell stage before 30\p=n-\31h after insemination developed into morula\p=n-\blastocyst. We suggest that the lag-phase as well as the link between early cleavage kinetics and further development could be related to the transcriptional activity of the embryo at about the 5\p=n-\8-cell stage.

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Section: Introductionmentioning

confidence: 99%

Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Grisart¹,

Massip²,

Dessy³

1994

Reproduction

124

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“…It means the transition from a program controlled by proteins synthesized from maternal mRNAs stored in the oocyte to a program controlled by proteins synthesized from the embryonic genome (Barnes and Eyestone, 1990). This transition normally occurs from the third to the fourth day as the embryo grows from 8 to 16 cells in the bovines.…”

Section: Discussionmentioning

confidence: 99%

“…Activin A is produced mainly by oviduct epithelial cells in bovines, so the addition of activin A to embryo culture medium in vitro may imitate the physiological environment in the oviduct in vivo (Gandolfi et al, 1995). Activin A is presently also known for its activity on the growth and differentiation of various cell types (Barnes and Eyestone, 1990;Hirai et al, 2007), is present in mammalian oviducts and may physiologically regulate the development of preimplantation embryos (Albano et al, 1993).…”

Section: Recombinant Human Activin a Promotes Development Of Bovine Smentioning

confidence: 99%

Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro

Shao¹,

Lan²,

Liu³

et al. 2010

CZECH J ANIM SCI

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ABSTRACT:To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage based on the traditional culture method, and analyzed the expression level of the genes related to blastocyst hatching (Na/K-ATPase, Glut-1) and related to activin A signalling pathway (ActRII and Smad2). We generated the bovine SCNT embryo using a Holstein cow oocyte as recipient cytoplasm and a foetal ear fibroblast (Holstein cow, 120 days) as donor cell. The embryos were cultured as follows: experiment 1, the addition of activin A at the concentrations of 0 (control), 20 (M1-20), 40 (M1-40) or 80 ng/ml (M1-80) to the media during the first three days and no addition during the subsequent 5 days; experiment 2, no addition of activin A to the media during the first 3 days and the addition of activin A at the concentrations of 0 (control), 20 (M2-20), 40 (M2-40) or 80 ng/ml (M2-80) during the subsequent 5 days. The results indicated that the blastocyst formation rate and hatching rate, and total blastomere numbers as well as ICM/TE obtained in experiment 1 were not significantly different from the control group (P > 0.05). In contrast, these values obtained in experiment 2 were significantly higher than in the control group (P < 0.05). In addition, the relative abundance (ratio to GAPDH mRNA) of each gene (Glut-1, ActR II and Smad2) was not significantly different among the treatments in the experiment. The expression levels of 4 genes (Na/K-ATPase, Glut-1, ActR II and Smad2) in blastocysts obtained in experiment 2 were higher than those obtained in experiment 1. In conclusion, the present study suggests that the addition of activin A to the culture media from day 4 to day 8 can enhance the developmental competence of bovine SCNT embryos.

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“…Recently, it was found that the effect of PCC was likely deleterious to mid to late S and G 2 nuclei (Collas et al, 1992a;Barnes et al, 1993;Campbell et al, 1993), resulting in abnormal chromosomal constitution and incorrect ploidy of reconstructed embryos due to DNA re-replication, as suggested by Campbell et al (1993). In blastomeres of early embryos, almost the whole cell cycle of these cells was occupied by the S phase (Barnes and Eyestone, 1990;Collas et al, 1992a). This explains the poor results of early nuclear transfer studies without cell cycle synchronization of donor cells (see review by Yang, 1991 (6 h) phase (Barnes and Eyestone, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…In blastomeres of early embryos, almost the whole cell cycle of these cells was occupied by the S phase (Barnes and Eyestone, 1990;Collas et al, 1992a). This explains the poor results of early nuclear transfer studies without cell cycle synchronization of donor cells (see review by Yang, 1991 (6 h) phase (Barnes and Eyestone, 1990). This suggests that in our experiment, membrane fusion, induced at 6-7 h after activation, occurred at G1 or early S phase of the recipient oocyte.…”

Section: Discussionmentioning

confidence: 99%

Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 h of age

Du¹,

Jiang²,

Yang³

1995

Reprod. Nutr. Dev.

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Summary ― This research was designed to study in vitro development of bovine nuclear transferred embryos using enucleated young in vitro matured oocytes 24 h of age as recipient cytoplasts activated prior to nuclear transfer. The oocytes were enucleated and then activated with electric pulse at 24 h of age followed by incubation with cycloheximide for 6-7 h before nuclear transfer and membrane fusion with a blastomere of the [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]

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Early cleavage and the maternal zygotic transition in bovine embryos

Cited by 161 publications

References 42 publications

Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Cinematographic analysis of bovine embryo development in serum-free oviduct-conditioned medium

Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro

Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 h of age

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