Early-cleavage is a reliable predictor for embryo implantation in the GnRH agonist protocols but not in the GnRH antagonist protocols

Yang, Wen-Jui; Hwu, Yuh‐Ming; Lee, Robert Kuo-Kuang; Li, Sheng-Hsiang; Fleming, Steven D.

doi:10.1186/1477-7827-7-20

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…The first cleavage can be examined with the breakdown of the pronuclear membrane (the start of the M phase of the first cell cycle) and directly with the presence of cleavage, because the duration of the M phase is relatively constant (3-4 hours) [ 30 , 31 ]. It is known that early cleavage is a strong indicator of the quality and the viability of the embryos [ 30 , 32 , 33 ], although a recent study showed higher implantational potential for early-cleavage embryos only with the use of GnRH agonists [ 34 ]. We observed significantly higher number of blastomers in the antagonist group on day 2, while the higher presence of early cleavage in this group did not reach statistical significance.…”

Section: Discussionmentioning

confidence: 99%

Impact of GnRH analogues on oocyte/embryo quality and embryo development in in vitro fertilization/intracytoplasmic sperm injection cycles: a case control study

Murber

Fancsovits

Ledó

et al. 2009

Reprod Biol Endocrinol

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Background: Despite the clinical outcomes of ovarian stimulation with either GnRH-agonist or GnRH-antagonist analogues for in vitro fertilization (IVF) being well analysed, the effect of analogues on oocyte/embryo quality and embryo development is still not known in detail. The aim of this case-control study was to compare the efficacy of a multiple-dose GnRH antagonist protocol with that of the GnRH agonist long protocol with a view to oocyte and embryo quality, embryo development and IVF treatment outcome.

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Section: Discussionmentioning

confidence: 99%

Impact of GnRH analogues on oocyte/embryo quality and embryo development in in vitro fertilization/intracytoplasmic sperm injection cycles: a case control study

Murber

Fancsovits

Ledó

et al. 2009

Reprod Biol Endocrinol

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“…Patients ( n = 40) undergoing intracytoplasmic sperm injection (ICSI) treatments at the Center of Reproductive Medicine, Mackay Memorial Hospital, Taiwan received controlled ovarian hyperstimulation using the gonadotropin-releasing hormone antagonist protocol as described previously [ 27 ]. All patients were younger than 38 years.…”

Section: Methodsmentioning

confidence: 99%

Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development

Lin

Hwu

et al. 2015

Reprod Biol Endocrinol

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BackgroundGJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyte maturation, fertilization, and early embryo development.MethodsIn total, 308 cumulus cell samples separated from individual cumulus–oocyte complex were obtained from 40 patients undergoing the intracytoplasmic sperm injection treatment procedure. Gene expression levels (mRNA levels) in cumulus cells were assessed using quantitative real-time polymerase chain reaction.ResultsGene expression levels of GJA1 and SERPINE2 in cumulus cells surrounding mature oocytes were significantly lower than those in cumulus cells enclosing immature oocytes. PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes. GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.ConclusionsGJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation. PRSS35 may be correlated with oocyte fertilization potential. However, GJA1, PRSS35, PTX3, and SERPINE2 may not be considered as marker genes for predicting embryo morphology.

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“…In vitro studies have shown that GNRH antagonist is responsible for an inhibitory effect on pre-implantation development of mouse embryos . In an attempt to explain such results, Yang et al (2009) have recently hypothesised that GNRH antagonists could interfere with cell growth by decreasing the synthesis of insulinlike growth factor (IGF) and epidermal growth factor receptors, which are involved in the MAP kinasemediated mitogenic cascade. However, the developmental potential of human pre-implantation embryos does not seem to be limited by putative detrimental effects of GNRH antagonist (Yang et al 2009).…”

Section: How Does Ovarian Stimulation Affect Early Oocyte and Embryo mentioning

confidence: 99%

“…In an attempt to explain such results, Yang et al (2009) have recently hypothesised that GNRH antagonists could interfere with cell growth by decreasing the synthesis of insulinlike growth factor (IGF) and epidermal growth factor receptors, which are involved in the MAP kinasemediated mitogenic cascade. However, the developmental potential of human pre-implantation embryos does not seem to be limited by putative detrimental effects of GNRH antagonist (Yang et al 2009). Additionally, high doses of GNRH antagonist were shown not to harm the implantation potential of embryos in frozenthawed cycles (Kol et al 1999), and a recent metaanalysis showed no significant differences in live birth rates following co-treatment with GNRH agonists versus GNRH antagonists (Kolibianakis et al 2006).…”

Section: How Does Ovarian Stimulation Affect Early Oocyte and Embryo mentioning

confidence: 99%

The impact of ovarian stimulation for IVF on the developing embryo

Santos¹,

Kuijk²,

Macklon³

2010

Reproduction

175

128

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The use of assisted reproductive technologies (ART) has been increasing over the past three decades, and, in developed countries, ART account for 1-3% of annual births. In an attempt to compensate for inefficiencies in IVF procedures, patients undergo ovarian stimulation using high doses of exogenous gonadotrophins to allow retrieval of multiple oocytes in a single cycle. Although ovarian stimulation has an important role in ART, it may also have detrimental effects on oogenesis, embryo quality, endometrial receptivity and perinatal outcomes. In this review, we consider the evidence for these effects and address possible underlying mechanisms. We conclude that such mechanisms are still poorly understood, and further knowledge is needed in order to increase the safety of ovarian stimulation and to reduce potential effects on embryo development and implantation, which will ultimately be translated into increased pregnancy rates and healthy offspring.

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Early-cleavage is a reliable predictor for embryo implantation in the GnRH agonist protocols but not in the GnRH antagonist protocols

Abstract: Background: To test if early-cleavage was a strong predictor of pregnancy in patients receiving either a GnRH agonist long protocol or a GnRH antagonist protocol for in-vitro fertilization treatment (IVF) and intracytoplasmic sperm injection (ICSI).

Cited by 8 publications

References 27 publications

Impact of GnRH analogues on oocyte/embryo quality and embryo development in in vitro fertilization/intracytoplasmic sperm injection cycles: a case control study

Impact of GnRH analogues on oocyte/embryo quality and embryo development in in vitro fertilization/intracytoplasmic sperm injection cycles: a case control study

Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development

The impact of ovarian stimulation for IVF on the developing embryo

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