Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation

Tzung, Keh-Weei; Goto, Ryoji; Saju, Jolly M.; Sreenivasan, Rajini; Saito, Taiju; Arai, Katsutoshi; Yamaha, Etsuro; Hossain, Mohammad Sorowar; Calvert, Meredith; Orbán, László

doi:10.1016/j.stemcr.2014.10.011

Cited by 152 publications

(119 citation statements)

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“…Furthermore, there is a strong correlation between the number of germ cells an individual has during gonadal development and their eventual sexual fate (X. G. Wang et al, 2007a, 2007b; Tzung et al, 2015). These data suggest that a threshold level of oocytes is required for ovarian fate and therefore female sex-determination.…”

Section: Discussionmentioning

confidence: 99%

“…In zebrafish, early germ cell numbers (counted prior to histologically apparent sex-differentiation) correlate with sex. Individuals with more germ cells were more likely to develop as females than males (Tzung et al, 2015). However, it is not clear whether this is a causative or consequential effect of sex determination, as the timing of sex determination in zebrafish is not well defined.…”

Section: Introductionmentioning

confidence: 99%

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Dmrt1 is necessary for male sexual development in zebrafish

Webster

Schach

Ordaz

et al. 2017

Developmental Biology

242

178

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The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is a key regulator of sex determination and/or gonadal sex differentiation across metazoan animals. This is unusual given that sex determination genes are typically not well conserved. The mechanisms by which zebrafish sex is determined have remained elusive due to the lack of sex chromosomes and the complex polygenic nature of sex determination in domesticated strains. To investigate the role of dmrt1 in zebrafish sex determination and gonad development, we isolated mutations disrupting this gene. We found that the majority of dmrt1 mutant fish develop as fertile females suggesting a complete male-to-female sex reversal in mutant animals that would have otherwise developed as males. A small percentage of mutant animals became males, but were sterile and displayed testicular dysgenesis. Therefore zebrafish dmrt1 functions in male sex determination and testis development. Mutant males had aberrant gonadal development at the onset of gonadal sex-differentiation, displaying reduced oocyte apoptosis followed by development of intersex gonads and failed testis morphogenesis and spermatogenesis. By contrast, female ovaries developed normally. We found that Dmrt1 is necessary for normal transcriptional regulation of the amh (anti-Müllerian hormone) and foxl2 (forkhead box L2) genes, which are thought to be important for male or female sexual development respectively. Interestingly, we identified one dmrt1 mutant allele that cooperates with a linked segregation distorter locus to generate an apparent XY sex determination mechanism. We conclude that dmrt1 is dispensable for ovary development but necessary for testis development in zebrafish, and that dmrt1 promotes male development by transcriptionally regulating male and female genes as has been described in other animals. Furthermore, the strong sex-ratio bias caused by dmrt1 reduction-of-function points to potential mechanisms through which sex chromosomes may evolve.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dmrt1 is necessary for male sexual development in zebrafish

Webster

Schach

Ordaz

et al. 2017

Developmental Biology

242

178

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show abstract

“…ZGLP1 is required for ovary or granulosa cell development in various species (34,35), suggesting that this DEG possibly plays a role in RI, which will expedite incipient SS; other DEGs are listed in Dataset S1.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome, genetic editing, and microRNA divergence substantiate sympatric speciation of blind mole rat, Spalax

Wang

Knisbacher³

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Incipient sympatric speciation in blind mole rat, Spalax galili, in Israel, caused by sharp ecological divergence of abutting chalkbasalt ecologies, has been proposed previously based on mitochondrial and whole-genome nuclear DNA. Here, we present new evidence, including transcriptome, DNA editing, microRNA, and codon usage, substantiating earlier evidence for adaptive divergence in the abutting chalk and basalt populations. Genetic divergence, based on the previous and new evidence, is ongoing despite restricted gene flow between the two populations. The principal component analysis, neighbor-joining tree, and genetic structure analysis of the transcriptome clearly show the clustered divergent two mole rat populations. Gene-expression level analysis indicates that the population transcriptome divergence is displayed not only by soil divergence but also by sex. Gene ontology enrichment of the differentially expressed genes from the two abutting soil populations highlights reproductive isolation. Alternative splicing variation of the two abutting soil populations displays two distinct splicing patterns. L-shaped F ST distribution indicates that the two populations have undergone divergence with gene flow. Transcriptome divergent genes highlight neurogenetics and nutrition characterizing the chalk population, and energetics, metabolism, musculature, and sensory perception characterizing the abutting basalt population. Remarkably, microRNAs also display divergence between the two populations. The GC content is significantly higher in chalk than in basalt, and stress-response genes mostly prefer nonoptimal codons. The multiple lines of evidence of ecologicalgenomic and genetic divergence highlight that natural selection overrules the gene flow between the two abutting populations, substantiating the sharp ecological chalk-basalt divergence driving sympatric speciation.natural selection | ecological adaptive speciation | DNA editing | microRNA regulation | nonoptimal codon usage

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“…Gene ontology terms of these differentially expressed genes included biological processes such as reproduction (GO:0000003), sexual reproduction (GO:0019953), the reproductive process (GO:0022414), the reproductive developmental process (GO:0003006), gamete generation (GO:0007276), fertilization (GO:0009566) and the reproductive cellular process (GO:0048610). Twenty-one of these transcripts were also shown to have sex-related roles or differential expression in the gonads in previous studies (Table 4) [8,[30][31][32][33][34]. This finding showed that the assembled transcriptome could be reliably used as a reference for differential expression analyses.…”

Section: Application Of the Asian Seabass Transcriptome For Rna-seq Ementioning

confidence: 54%

Transcriptome Survey of a Marine Food Fish: Asian Seabass (Lates calcarifer)

Thevasagayam

Sridatta

Jiang

et al. 2015

JMSE

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The Asian seabass (or barramundi; Lates calcarifer) is a marine teleost and a popular food fish in Southeast Asia and Australia. To date, comprehensive genome and OPEN ACCESS J. Mar. Sci. Eng. 2015, 3 383 transcriptome sequence information has not been available for this species in public repositories. Here, we report a comprehensive de novo transcriptome assembly of the Asian seabass. These data will be useful for the development of molecular tools for use in aquaculture of Asian seabass as well as a resource for genome annotation. The transcriptome was obtained from sequences generated from organs of multiple individuals using three different next-generation sequencing platforms (454-FLX Titanium, SOLiD 3+, and paired-end Illumina HiSeq 2000). The assembled transcriptome contains >80% of the expected protein-coding loci, with 58% of these represented by a predicted full-length cDNA sequence when compared to the available Nile tilapia RefSeq dataset. Detailed descriptions of the various steps involved in sequencing and assembling a transcriptome are provided to serve as a helpful guide for transcriptome projects involving de novo assembly of short sequence reads for non-model teleosts or any species of interest.

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Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation

Cited by 152 publications

References 50 publications

Dmrt1 is necessary for male sexual development in zebrafish

Dmrt1 is necessary for male sexual development in zebrafish

Transcriptome, genetic editing, and microRNA divergence substantiate sympatric speciation of blind mole rat, Spalax

Transcriptome Survey of a Marine Food Fish: Asian Seabass (Lates calcarifer)

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