Early diagnosis of a developing biosystem using acousto-optic imaging

Abstract

Studying the embryo development at an early stage is considered. For this, hyperspectral imaging using an acousto-optical microscope-based system is proposed. Acousto-optic visualization of the early developmental stages of the loach Misgurnus fossilis embryo showed a regular change in the spectral characteristics of different image areas corresponding to the tissues and body fluids, depending on the functional state of the embryo. We show that there is a discrete set of maxima in the optical absorption spectrum with a distance of 20 nm and 30 nm between them. Value 20 nm corresponds to the normal development of biological tissue. The appearance of the value 30 nm between the maxima of neighboring optical absorption bands indicates the development of pathological processes in the biosystem. Such deviations from the norm are characteristic of germinal tissues, and they are absent in the perivitelline fluid. Thus, we demonstrated that for the early diagnosis of the physiological state of a developing biosystem, special attention should be paid to optical absorption spectra.