Early diagnosis of dengue in travelers: Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology

Huhtamo, Eili; Hasu, Essi; Uzcátegui, Nathalie Y.; Erra, Elina; Nikkari, Simo; Kantele, Anu; Vapalahti, Olli; Piiparinen, Heli

doi:10.1016/j.jcv.2009.11.001

Cited by 116 publications

(91 citation statements)

References 16 publications

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“…NS1 rapid test has been proven useful in screening travelers in airports. 27,40 Our study further extends utility of NS1 in dengue diagnosis in travelers 27,40,41 by using a broad range of patients with different immune profiles (primary and secondary) and serum samples obtained at different phases of disease.…”

Section: Discussionmentioning

confidence: 66%

Detection of Dengue Virus Nonstructural Protein 1 (NS1) by Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers

et al. 2013

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Background. Dengue virus ( DENV) nonstructural protein 1 ( NS1) has been used as a novel diagnostic marker during the early phase of DENV infection. Methods. Presence of NS1 antigen was examined using 336 serum samples obtained from 276 travelers returning to Japan from Asia, Central and South America, Pacific Islands, and Africa with dengue. Assay specificity was evaluated using 148 non-dengue samples. Results. Positive rates among four DENV serotypes were 68%-89%. NS1 antigen positive rates were at similar levels between primary infection and secondary infection. NS1 antigen positive rates were 88%-96% on days 1-5, 75%-100% on days 6-10, and 36-60% on ≥day 11. Positive rates using real-time polymerase chain reaction (RT-PCR) were over 70% on days 1-5, but decreased thereafter. Conclusions.The results indicate that NS1 antigen positive rates were higher than those of RT-PCR during longer period of early phase in DENV infection. Thus, NS1 antigen ELISA is a useful tool for confirming DENV infection in international travelers, when it is used in combination with anti-DENV IgM ELISA.

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Section: Discussionmentioning

confidence: 66%

Detection of Dengue Virus Nonstructural Protein 1 (NS1) by Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers

et al. 2013

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“…1). Additional tests revealed that flavivirus antibody-positive human sera were reactive with LAMV and ILOV antigens, including pooled WNV-positive human sera ( n =4) and human sera from patients known to have been infected with any one of the four DENV serotypes ( n =9) (Huhtamo et al, 2010). The antibody tests suggested that ILOV was more antigenically reactive with the flavivirus-antibody-positive human sera than LAMV as determined by serial serum titration comparisons (Supplementary table).…”

Section: Resultsmentioning

confidence: 99%

Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses

et al. 2014

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Novel flaviviruses that are genetically related to pathogenic mosquito-borne flaviviruses (MBFV) have been isolated from mosquitoes in various geographical locations, including Finland. We isolated and characterized another novel virus of this group from Finnish mosquitoes collected in 2007, designated as Ilomantsi virus (ILOV). Unlike the MBFV that infect both vertebrates and mosquitoes, the MBFV-related viruses appear to be specific to mosquitoes similar to the insect-specific flaviviruses (ISFs). In this overview of MBFV-related viruses we conclude that they differ from the ISFs genetically and antigenically. Phylogenetic analyses separated the MBFV-related viruses isolated in Africa, the Middle East and South America from those isolated in Europe and Asia. Serological cross-reactions of MBFV-related viruses with other flaviviruses and their potential for vector-borne transmission require further characterization. The divergent MBFV-related viruses are probably significantly under sampled to date and provide new information on the variety, properties and evolution of vector-borne flaviviruses.

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“…This finding contrasts with dengue virus infections when the viremic phase overlaps the period when the IgM is detectable. 12 Interestingly, the cross-reactive IgM response to West Nile virus, another flavivirus, was not seen for samples from this patient. A similar lack of cross-reactivity to West Nile virus was noted in the Yap Island outbreak, 13 and the case of the recent German traveler.…”

mentioning

confidence: 76%

First Case of Zika Virus Infection in a Returning Canadian Traveler

Fonseca¹,

Meatherall²,

Zarra³

et al. 2014

The American Society of Tropical Medicine and Hygiene

216

178

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A woman who recently traveled to Thailand came to a local emergency department with a fever and papular rash. She was tested for measles, malaria, and dengue. Positive finding for IgM antibody against dengue and a failure to seroconvert for IgG against dengue for multiple blood samples suggested an alternate flavivirus etiology. Amplification of a conserved region of the non-structural protein 5 gene of the genus Flavivirus yielded a polymerase chain reaction product with a matching sequence of 99% identity with Zika virus. A urine sample and a nasopharygeal swab specimen obtained for the measles investigation were also positive for this virus by reverse transcription polymerase chain reaction. Subsequently, the urine sample yielded a Zika virus isolate in cell culture. This case report describes a number of novel clinical and laboratory findings, the first documentation of this virus in Canada, and the second documentation from this region in Thailand.

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Early diagnosis of dengue in travelers: Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology

Cited by 116 publications

References 16 publications

Detection of Dengue Virus Nonstructural Protein 1 (NS1) by Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers

Detection of Dengue Virus Nonstructural Protein 1 (NS1) by Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers

Novel flaviviruses from mosquitoes: Mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses

First Case of Zika Virus Infection in a Returning Canadian Traveler

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