Early exposure to caffeine affects gene expression of adenosine receptors, DARPP-32 and BDNF without affecting sensibility and morphology of developing zebrafish (Danio rerio)

Capiotti, Katiúcia Marques; Menezes, Fabiano Peres; Nazario, Luiza Reali; Pohlmann, Julhana Bianchini; Oliveira, Giovanna Medeiros Tavares de; Fazenda, Lidiane; Bogo, Maurı́cio Reis; Bonan, Carla Denise; Silva, Rosane Souza da

doi:10.1016/j.ntt.2011.08.010

Cited by 54 publications

(36 citation statements)

References 34 publications

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“…In interpreting these results, it is also necessary to take into account that about half of the patients received oral caffeine during detoxification. Although BDNF is increased after caffeine administration to zebrafish whose embryos were previously treated with caffeine (Capiotti et al, 2011) or in caffeine-treated sleep-deprived rats (Alhaider et al, 2011), we did not find differences in BDNF serum levels between caffeine-and placebotreated abstinent cocaine addicts. This may be due to the predominance of cocaine withdrawal over the stimulant effects of caffeine, an antagonist of adenosine receptors, during the first two abstinence weeks.…”

Section: Discussioncontrasting

confidence: 68%

Brain-derived neurotrophic factor serum levels in cocaine-dependent patients during early abstinence

Corominas-Roso

Roncero

Eiroá-Orosa

et al. 2013

European Neuropsychopharmacology

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Cite as: Corominas-Roso, M., Roncero, C., Eiroa-Orosa, F. J., Gonzalvo, B., Grau-Lopez, L., Ribases, M., … Casas, M. (2013). Brain-derived neurotrophic factor serum levels in cocaine-dependent patients during early abstinence. European Neuropsychopharmacology, 23(9) completed a two-week detoxification program at an inpatient facility. Two serum samples were collected for each patient at baseline and at the end of the protocol.Serum samples were also collected for 46 healthy controls (aged 35.52 ± 9.37 years).Demographic, consumption and clinical data were recorded for all patients.Significantly lower serum BDNF levels (p<.0001) were observed for cocaine-dependent patients at baseline compared to healthy controls. Serum BDNF levels increased significantly across 12 days of early abstinence (p = .030). Baseline BDNF levels correlated with craving (p = .034). Post-detoxification BDNF levels correlated with craving (p = .018), loss of control (p < .000), abstinence measures (p = 0.031), depression (p = 0.036), and anxiety (p = 0.036). Post-detoxification BDNF levels also had predictive value for the loss of control measure of craving. Chronic cocaine use is associated with decreased serum BDNF. A progressive increase in serum BDNF levels during early abstinence correlates with cocaine craving and abstinence symptoms and may reflect increasing BDNF levels in different brain regions. These findings suggest that serum BDNF may be a biomarker for cocaine addiction.

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Section: Discussioncontrasting

confidence: 68%

Brain-derived neurotrophic factor serum levels in cocaine-dependent patients during early abstinence

Corominas-Roso

Roncero

Eiroá-Orosa

et al. 2013

European Neuropsychopharmacology

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“…Quantitative PCR was performed using SYBR ® Green I (Invitrogen) to detect double-strand cDNA synthesis. Reactions were done in a volume of 25 μL using 12.5 μL of diluted cDNA, containing SYBR ® Green I 0.2 times diluted (Invitrogen), 100 μM dNTP, 1× PCR buffer, 3 mM MgCl 2 , 0.25 U Platinum ® Taq DNA Polymerase (Invitrogen), and 200 nM of each reverse and forward primers (Table 1) [35][36][37][38]. The PCR cycling conditions were an initial polymerase activation step for 5 min at …”

Section: Gene Expression Analysis By Quantitative Real-time Reverse Tmentioning

confidence: 99%

Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Capiotti

Siebel

Kist

et al. 2016

Purinergic Signalling

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Hyperglycemia is the main feature for the diagnosis of diabetes mellitus (DM). Some studies have demonstrated the relationship between DM and dysfunction on neurotransmission systems, such as the purinergic system. In this study, we evaluated the extracellular nucleotide hydrolysis and adenosine deamination activities from encephalic membranes of hyperglycemic zebrafish. A significant decrease in ATP, ADP, and AMP hydrolyses was observed at 111-mM glucose-treated group, which returned to normal levels after 7 days of glucose withdrawal. A significant increase in ectoadenosine deaminase activity was observed in 111-mM glucose group, which remain elevated after 7 days of glucose withdrawal. The soluble-adenosine deaminase activity was significantly increased just after 7 days of glucose withdrawal. We also evaluated the gene expressions of ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases), ecto-5′-nucleotidase, ADA, and adenosine receptors from encephala of adult zebrafish. The entpd 2a.1, entpd 2a.2, entpd 3, and entpd 8 mRNA levels from encephala of adult zebrafish were decreased in 111-mM glucose-treated and glucose withdrawal groups. The gene expressions of adenosine receptors (adora 1 , adora 2aa , adora 2ab , and adora 2b ) were decreased in 111-mM glucose-treated and glucose withdrawal groups. The gene expression of ADA (ada 2a.1) was decreased in glucose withdrawal group. Maltodextrin, used as a control, did not affect the expression of adenosine receptors, ADA and E-NTPDases 2, 3, and 8, while the expression of ecto-5′-nucleotidase was slightly increased and the E-NTPDases 1 decreased. These findings demonstrated that hyperglycemia might affect the ecto-nucleotidase and adenosine deaminase activities and gene expression in zebrafish, probably through a mechanism involving the osmotic effect, suggesting that the modifications caused on purinergic system may also contribute to the diabetes-induced progressive cognitive impairment.

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“…Adult (<8 months old) male wild type zebrafish with Tuebingen background (Capiotti et al, 2011;Maximino, Lima, Olivera, Picanço-Diniz, & Herculano, 2011) were obtained from a local supplier (Redfish Agroloja, RS, Brazil). Animals were maintained in 20 L housing tanks, divided into six compartments and filled with tap water previously treated with Tetra's AquaSafe Ò (Blacksburg, VA) to neutralize chlorine, chloramines, and heavy metals present in the water that could be harmful to the fish.…”

Section: Animals and Housingmentioning

confidence: 99%

Y-Maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods

Cognato

Bortolotto

Blazina

et al. 2012

Neurobiology of Learning and Memory

158

132

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Early exposure to caffeine affects gene expression of adenosine receptors, DARPP-32 and BDNF without affecting sensibility and morphology of developing zebrafish (Danio rerio)

Cited by 54 publications

References 34 publications

Brain-derived neurotrophic factor serum levels in cocaine-dependent patients during early abstinence

Brain-derived neurotrophic factor serum levels in cocaine-dependent patients during early abstinence

Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Y-Maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods

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