Early Infective Endocarditis Due to Staphylococcus aureus Following Dental Procedures

Kasmi, Gentian; Refatllari, Etleva; Dumani, Selman; Refatllari, Ali

doi:10.7754/clin.lab.2014.140502

Cited by 6 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although bacteria from the HACEK group were not discovered with 16S PCR IE, they should be covered by a POCT system, despite their rareness (Table 2). The detection of 5 staphylococcal cases using multiplex PCR assay is not surprising, as Staphylococcus aureus is described as the most common cause of IE in the developed world [11]. There was only a slight difference in the absolute numbers of staphylococcal cases using 16S rDNA PCR/sequencing (6 cases, although Staphylococcus spp in 6 cases.…”

Section: Discussionmentioning

confidence: 98%

Proof of Concept Evaluation of a New Diagnostic POCT Device for Culture- Independent Microbiological Diagnosis of Infective Endocarditis

Karrasch¹,

Pfister²,

Diab³

et al. 2019

Arch Clin Microbiol

View full text Add to dashboard Cite

Introduction: Accurate and fast microbiological diagnosis of infective endocarditis (IE) is of vital importance for patient outcome.Material & Methods: Forty culture-negative heart valves were evaluated with a new POCT multiplex-PCR cartridge (Unyvero ™ , Curetis AG, Holzgerlingen, Germany), advertised to detect several Gram positive/negative bacteria and fungi, together with several antibiotic resistance genes. Those POCT results were compared to conventional 16S rDNA PCR/sequencing results. Results: POCT multiplex-PCR was positive in 13 cases [Staphylococcus aureus (n=5), Enterococcus spp. / E. faecalis (n=5), ConS (n=1), Granulicatella adjacens (n=1), Abiotrophia adjacens (n=1)]. Antibiotic resistances were found in 44 specimens, from which 2 specimens were without any pathogen identification. 16S rDNA PCR was positive in 20 cases. Consecutive sequencing identified those as Staphylococcus spp. (n=6), Enterococcus faecalis (n=4), Streptococcus spp. (n=4), Leifsonia shinshuensis (n=1),Granulicatella elegans / G. adjacens (n=2), Abiotrophia adjacens (n=1). One case was positive in 16S PCR without any reliable signal in sequencing. When comparing both methods, identification was consistent in 9 cases and divergent in other 9 cases. Discussion: This POCT cartridge is easy to integrate into the daily microbiology laboratory work flow, and is less laborious than 16S sequencing PCR. For the application in routine IE diagnosis, the system needs to be optimized to include targets for viridans streptococci and HACEK group. In addition, problems with invalid resistance and pathogen target detection need to be fixed by the producer. Conclusion:The analyzed POCT system might be a future diagnostic tool for IE detection following assay optimization.

show abstract

Section: Discussionmentioning

confidence: 98%

Proof of Concept Evaluation of a New Diagnostic POCT Device for Culture- Independent Microbiological Diagnosis of Infective Endocarditis

Karrasch¹,

Pfister²,

Diab³

et al. 2019

Arch Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Daneben werden auch Staphylokokken als ursächlich angesehen, Staphylococcus aureus sogar als die häufigste Ursache für die IE in vielen Regionen der Industrieländer [28].…”

Section: Merkeunclassified

Zahnsanierung vor Herzklappenersatz – ein CME-Artikel der S2k-Leitlinie

Ritschl¹,

Wolff²,

Deppe³

2019

Zahnmedizin up2date

View full text Add to dashboard Cite

“…Zatiaľ čo dôležitosť stafylokokov ako medicínsky významných patogénov je známa už mnoho rokov, existuje pomerne málo detailných štúdií o distribúcii stafylokokov v orálnej dutine. Je zjavné, že ústna dutina môže byť popri nazálnej dutine dôležitým rezervoárom stafylokokových infekcií -vrátane meticilín rezistentných, ktoré za istých podmienok môžu spôsobiť závažné ochorenia organizmu, vrátane infekčnej endokarditídy [11]. Meticilín rezistentné kmene S. aureus (MRSA), ktoré vykazujú mnohopočetnú rezistenciu voči bežne používaným antibiotikám sú jedným z najvýznamnejších gram-pozitívnych rezistentných patogénov.…”

Section: úVod a Cieľ šTúdieunclassified

“…Podľa doporučení tejto organizácie, ako aj Inštitútu pre klinické a laboratórne štandardy [5] cov, vrátane meticilín rezistentných kmeňov, je okrem iných orgánov, ako je koža, hltan, sliznica nosa apod., aj dutina ústna [2,4,16]. Navyše, ako naznačujú ďalšie štúdie, S. aureus sa môže pravdepodobne z ústnej dutiny diseminovať aj do iných častí tela a spôsobiť tu závažné infekcie [11,21]. Čo sa týka zisteného percenta pozitívnych nálezov v nosovej dutine, naše výsledky sú podobné tým, ktoré zistili Mehraj a kol.…”

Section: Diskový Difúzny Testunclassified

Oral Carriage of Staphylococcus aureus among Dental Patients in Dependence on Conditions in Oral Cavity

Kaiglová¹,

Špajdelová²,

Stanko³

et al. 2016

Czech Dental Journal

View full text Add to dashboard Cite

Early Infective Endocarditis Due to Staphylococcus aureus Following Dental Procedures

Cited by 6 publications

References 0 publications

Proof of Concept Evaluation of a New Diagnostic POCT Device for Culture- Independent Microbiological Diagnosis of Infective Endocarditis

Proof of Concept Evaluation of a New Diagnostic POCT Device for Culture- Independent Microbiological Diagnosis of Infective Endocarditis

Zahnsanierung vor Herzklappenersatz – ein CME-Artikel der S2k-Leitlinie

Oral Carriage of Staphylococcus aureus among Dental Patients in Dependence on Conditions in Oral Cavity

Contact Info

Product

Resources

About