Early life supplementation with a natural blend containing turmeric, thymol, and yeast cell wall components to optimize rumen anatomical and microbiological development and productivity in dairy goats

Belanche, Alejandro; Árturo-Schaan, M.; Leboeuf, L.; Yáñez-Ruíz, D. R.; Martín-García, A. I.

doi:10.3168/jds.2022-22621

Cited by 5 publications

(1 citation statement)

References 62 publications

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“…The ability of anaerobic fungi to form resistant spores that allow them to retain viability in dung, soil and feed, may explain the similar anaerobic fugal concentration and diversity observed across treatments ( 64 ). Moreover this anaerobic fungal concentration was higher (2 logs) than we previously reported in ruminants fed concentrate diets suggesting an active role in fiber degradation ( 65 ). Anaerobic fungi, due to their long life cycle and they ability to digest recalcitrant lignocellulosic substrates, are efficient feed degraders ( 66 ) and able to penetrate the plant cuticle providing additional sites for bacteria to attach to protected plant tissues ( 60 ).…”

Section: Discussionmentioning

confidence: 45%

Enhancing rumen microbial diversity and its impact on energy and protein metabolism in forage-fed goats

Belanche,

Palma-Hidalgo,

Jiménez

et al. 2023

Front. Vet. Sci.

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IntroductionThis study explores if promoting a complex rumen microbiota represents an advantage or a handicap in the current dairy production systems in which ruminants are artificially reared in absence of contact with adult animals and fed preserved monophyte forage.MethodsIn order to promote a different rumen microbial diversity, a total of 36 newborn goat kids were artificially reared, divided in 4 groups and daily inoculated during 10 weeks with autoclaved rumen fluid (AUT), fresh rumen fluid from adult goats adapted to forage (RFF) or concentrate (RFC) diets, or absence of inoculation (CTL). At 6 months of age all animals were shifted to an oats hay diet to determine their ability to digest a low quality forage.Results and discussionEarly life inoculation with fresh rumen fluid promoted an increase in the rumen overall microbial diversity which was detected later in life. As a result, at 6 months of age RFF and RFC animals had higher bacterial (+50 OTUs) and methanogens diversity (+4 OTUs) and the presence of a complex rumen protozoal community (+32 OTUs), whereas CTL animals remained protozoa-free. This superior rumen diversity and presence of rumen protozoa had beneficial effects on the energy metabolism allowing a faster adaptation to the forage diet, a higher forage digestion (+21% NDF digestibility) and an energetically favourable shift of the rumen fermentation pattern from acetate to butyrate (+92%) and propionate (+19%) production. These effects were associated with the presence of certain rumen bacterial taxa and a diverse protozoal community. On the contrary, the presence of rumen protozoa (mostly Entodinium) had a negative impact on the N metabolism leading to a higher bacterial protein breakdown in the rumen and lower microbial protein flow to the host based on purine derivatives urinary excretion (-17% to -54%). The inoculation with autoclaved rumen fluid, as source of fermentation products but not viable microbes, had smaller effects than using fresh inoculum. These findings suggest that enhancing rumen microbial diversity represents a desirable attribute when ruminants are fed forages in which the N supply does not represent a limiting factor for the rumen microbiota.

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Section: Discussionmentioning

confidence: 45%

Enhancing rumen microbial diversity and its impact on energy and protein metabolism in forage-fed goats

Belanche,

Palma-Hidalgo,

Jiménez

et al. 2023

Front. Vet. Sci.

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Co-fermented broth of forage grasses and Chinese herbal medicines improves rumen fermentation, gastrointestinal development, and microbial community in fattening lambs

Li,

Zhang,

Guo

et al. 2024

Preprint

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Digestive problems in fattening lambs have been concerned in recent years. Our study aimed to investigate the effects of a co-fermented broth (FB) from 4 forage grasses and 4 Chinese medicines on rumen fermentation, gastrointestinal development, and microbial community in lambs via in vitro and in vivo tests. In vitro fermentation of 48 h was performed to determine fermentation parameters (gas production, volatile fatty acid, ammonia nitrogen, etc.) and nutrient disappearance rate at various doses of FB supplements, and optimal doses of FB were used for in vivo test for a 120-d period. Ninety weaned lambs were assigned into 3 groups, offered FB in drinking water at 0, 1:500, and 1:1000, and fermentation characteristics, microbial community, and microstructures in rumen and jejunum were evaluated. Results showed that FB improved microbial community composition and diversity via analysis of phylum, family, and genus levels. Particularly, 1:500 FB increased abundances of Firmicutes and Muribaculaceae in rumen, and Actinomycetes and Aeriscardoviain jejunum, while decreased Euryarchaeota and Methanobrevibacter abundances. Meanwhile, FB demonstrated a positive influence in epithelial development of digestive tract, exhibiting great superficial areas of rumen papilla and jejunum villus. Our results suggested that FB supplement could contribute to fermentation and microecological balance.

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Changes in the growth performance, serum biochemistry, rumen fermentation, rumen microbiota community, and intestinal development in weaned goats during rumen-protected methionine treatment

Wang,

Liu,

et al. 2024

Front. Vet. Sci.

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Rumen-protected methionine (RPM) such as coated methionine (CM) and 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester (HMBi) was usually used in dairy cows, but how RPM affects meat goats remains unclear. In this study, thirty weaned male Jianzhou Da’er goats were randomly assigned to one of three treatments: fed basal diet or basal diet supplemented with 0.12% CM or 0.22% HMBi, with the aim of examining their impact on growth performance, serum biochemistry, rumen fermentation, rumen microbiota, and intestinal development in meat goats. The findings indicate that HMBi supplementation led to an increase in body weight, feed intake, and feed-to-gain ratio, whereas CM only resulted in an increase in feed intake (all p < 0.05). Both CM and HMBi resulted in an increase in serum total cholesterol (TC), blood urea nitrogen (BUN), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), albeit with a decrease in serum triglycerides (TG) and β-hydroxybutyric acid (BHB, all p < 0.05). Both CM and HMBi supplementation decreased the rumen butyric acid concentration (both p < 0.05). The 16S rRNA sequencing showed that HMBi supplementation significantly increased the total abundance of Bacteroidetes and Firmicutes. Both CM and HMBi supplements increased the abundance of Rikenella and Proteiniphilum but decreased the abundance of Eisenbergiella, Enterocloster, Massilioclostridium, Eubacterium, Angelakisella, Blastopirellula, Christensenella, and Pseudoruminococcus. CM supplementation specifically increased the abundance of Desulfobulbus, Sodaliphilus, and Coprococcus while decreasing the prevalence of Anaerocella, Mogibacterium, and Collinsella. The supplementation of HMBi significantly enhanced the abundance of Paraprevotella, Bacilliculturomica, Lachnoclostridium, Dysosmobacter, Barnesiella, and Paludibacter, while decreasing the abundance of Butyrivibrio and Pirellula. Moreover, the administration of both CM and HMBi supplementation resulted in an increase in the ammonia-producing and sulfate-reducing bacteria, whereas a decrease was observed in the ammonia-oxidating, health-associated, and disease-associated bacteria. Correlational analysis revealed that TG and BHB had a positive correlation with disease-associated and ammonia-oxidating bacteria, whereas they had a negative correlation with ammonia-producing bacteria. The serum BUN, ALP, and AST were positively correlated with ammonia-producing bacteria but were negatively correlated with ammonia-oxidating bacteria. Furthermore, both CM and HMBi supplementation improve the development of the small intestine, with HMBi having a better effect. In summary, this study indicates that both CM and HMBi supplementation improve lipid metabolism, nitrogen utilization, and intestinal development. The growth promotion effect of HMBi supplementation may be attributed to the increased abundance of volatile fatty acid-producing and nitrogen-utilizing bacteria and improved intestinal development.

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Early life supplementation with a natural blend containing turmeric, thymol, and yeast cell wall components to optimize rumen anatomical and microbiological development and productivity in dairy goats

Cited by 5 publications

References 62 publications

Enhancing rumen microbial diversity and its impact on energy and protein metabolism in forage-fed goats

Enhancing rumen microbial diversity and its impact on energy and protein metabolism in forage-fed goats

Co-fermented broth of forage grasses and Chinese herbal medicines improves rumen fermentation, gastrointestinal development, and microbial community in fattening lambs

Changes in the growth performance, serum biochemistry, rumen fermentation, rumen microbiota community, and intestinal development in weaned goats during rumen-protected methionine treatment

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