Early Molecular Events during Response to Oxidative Stress in Human Cells by Differential Proteomics

Tell, Gianluca

doi:10.1002/0471973122.ch13

Cited by 4 publications

(3 citation statements)

References 80 publications

(101 reference statements)

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“…A plausible explanation for the co-expression of these two functional groups is that the cytoskeleton is a major target of ROS and reactive nitrogen species (Dalle-Donne et al, 2001;Tell, 2006). Under conditions of oxidative stress, ROS-sensitive cysteine residues of actin either form intermolecular disulfide bridges or undergo reversible Sglutathionylation, which may confer protection against ROS (DalleDonne et al, 2003a;Dalle-Donne et al, 2003b).…”

Section: Discussionmentioning

confidence: 99%

Proteomic response to elevated PCO2 level in eastern oysters, Crassostrea virginica: evidence for oxidative stress

Tomanek

Zuzow

Ivanina

et al. 2011

Journal of Experimental Biology

252

178

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SUMMARYEstuaries are characterized by extreme fluctuations in CO 2 levels due to bouts of CO 2 production by the resident biota that exceed its capacity of CO 2 consumption and/or the rates of gas exchange with the atmosphere and open ocean waters. Elevated partial pressures of CO 2 (P CO2; i.e. environmental hypercapnia) decrease the pH of estuarine waters and, ultimately, extracellular and intracellular pH levels of estuarine organisms such as mollusks that have limited capacity for pH regulation. We analyzed proteomic changes associated with exposure to elevated P CO2 in the mantle tissue of eastern oysters (Crassostrea virginica) after 2weeks of exposure to control (~39Pa P CO2 ) and hypercapnic (~357Pa P CO2 ) conditions using two-dimensional gel electrophoresis and tandem mass spectrometry. Exposure to high P CO2 resulted in a significant proteome shift in the mantle tissue, with 12% of proteins (54 out of 456) differentially expressed under the high P CO2 compared with control conditions. Of the 54 differentially expressed proteins, we were able to identify 17. Among the identified proteins, two main functional categories were upregulated in response to hypercapnia: those associated with the cytoskeleton (e.g. several actin isoforms) and those associated with oxidative stress (e.g. superoxide dismutase and several peroxiredoxins as well as the thioredoxin-related nucleoredoxin). This indicates that exposure to high P CO2 (~357Pa) induces oxidative stress and suggests that the cytoskeleton is a major target of oxidative stress. We discuss how elevated CO 2 levels may cause oxidative stress by increasing the production of reactive oxygen species (ROS) either indirectly by lowering organismal pH, which may enhance the Fenton reaction, and/or directly by CO 2 interacting with other ROS to form more free radicals. Although estuarine species are already exposed to higher and more variable levels of CO 2 than other marine species, climate change may further increase the extremes and thereby cause greater levels of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Proteomic response to elevated PCO2 level in eastern oysters, Crassostrea virginica: evidence for oxidative stress

Tomanek

Zuzow

Ivanina

et al. 2011

Journal of Experimental Biology

252

178

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“…The increase in a number of tubulin isoforms in M. galloprovincialis at 24°C and 28°C and the subsequent decrease at 32°C (Fig.2, cluster III) illustrates a similar response. Although their importance in contributing to the heat shock response is recognized, the nature and functional relevance of cytoskeletal changes in response to heat and oxidative stress is still poorly understood (Dalle-Donne et al, 2001;Tell, 2006). Increased synthesis of sHsp (Figs2 and 3) can be triggered through the ROS-mediated denaturation of cytoskeletal proteins (Arrigo, 2007;Huot et al, 1996;Lavoie et al, 1995).…”

Section: Hypotheses Integrating the Observed Heat-stress-induced Chanmentioning

confidence: 99%

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

Tomanek

Zuzow

2010

Journal of Experimental Biology

272

273

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SUMMARYThe Mediterranean blue mussel, Mytilus galloprovincialis, an invasive species in California, has displaced the more heat-sensitive native congener, Mytilus trossulus, from its former southern range, possibly due to climate change. By comparing the response of their proteomes to acute heat stress we sought to identify responses common to both species as well as differences that account for greater heat tolerance in the invasive. Mussels were acclimated to 13°C for four weeks and exposed to acute heat stress (24°C, 28°C and 32°C) for 1h and returned to 13°C to recover for 24h. Using two-dimensional gel electrophoresis and tandem mass spectrometry we identified 47 and 61 distinct proteins that changed abundance in M. galloprovincialis and M. trossulus, respectively. The onset temperatures of greater abundance of some members of the heat shock protein (Hsp) 70 and small Hsp families were lower in M. trossulus. The abundance of proteasome subunits was lower in M. galloprovincialis but greater in M. trossulus in response to heat. Levels of several NADH-metabolizing proteins, possibly linked to the generation of reactive oxygen species (ROS), were lower at 32°C in the cold-adapted M. trossulus whereas proteins generating NADPH, important in ROS defense, were higher in both species. The abundance of oxidative stress proteins was lower at 32°C in M. trossulus only, indicating that its ability to combat heat-induced oxidative stress is limited to lower temperatures. Levels of NADdependent deacetylase (sirtuin 5), which are correlated with lifespan, were lower in M. trossulus in response to heat stress. In summary, the expression patterns of proteins involved in molecular chaperoning, proteolysis, energy metabolism, oxidative damage, cytoskeleton and deacetylation revealed a common loci of heat stress in both mussels but also showed a lower sensitivity to high-temperature damage in the warm-adapted M. galloprovincialis, which is consistent with its expanding range in warmer waters. Supplementary material available online at

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“…ROS/RNS can alter all biomolecules, but proteins are particularly sensitive due to the presence of redox susceptible cysteines (Cys) (Ying et al, 2007). ROS-mediated posttranslational modifications play key roles in signalling during normal cell growth (Eaton, 2006;Tell, 2006;Ying et al, 2007), but when ROS levels increase, oxidative damage can cause serious harm or even death of the cell/organisms (Dalle-Donne et al, 2006;Davies, 2005;Eaton, 2006;Ying et al, 2007).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Redox and global interconnected proteome changes in mice exposed to complex environmental hazards surrounding Doñana National Park

Michán

Chicano-Gálvez

Fuentes-Almagro

et al. 2019

Environmental Pollution

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Early Molecular Events during Response to Oxidative Stress in Human Cells by Differential Proteomics

Cited by 4 publications

References 80 publications

Proteomic response to elevated PCO2 level in eastern oysters, Crassostrea virginica: evidence for oxidative stress

Proteomic response to elevated PCO2 level in eastern oysters, Crassostrea virginica: evidence for oxidative stress

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

Redox and global interconnected proteome changes in mice exposed to complex environmental hazards surrounding Doñana National Park

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