2014
DOI: 10.1007/s13105-013-0280-5
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Early septic shock induces loss of oxidative phosphorylation yield plasticity in liver mitochondria

Abstract: We aimed to study the change in mitochondrial oxidative phosphorylation efficiency occurring at the early stage of septic shock in an experimental model. Thirty-six male Wistar rats were divided into two groups. In the first group, a cecal ligation and puncture (CLP) was carried out to induce septic shock for 5 h. The second group includes sham-operated rats and constitutes the control group. Blood gas analysis, alanine amino transferase, and lactic acid dosages were assayed 5 h after surgery. Liver mitochondr… Show more

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Cited by 15 publications
(28 citation statements)
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“…However, contrary to what could be expected, this was not associated with an increase in oxidative phosphorylation efficiency ATP/O, which could have compensated for energy production (ATP generation) from mitochondrial respiratory chain oxidative loss. This result reinforces and extends previous data [25] on the failure of the mitochondrial oxidative phosphorylation system to adjust its intrinsic coupling efficiency in early septic shock. Furthermore, in line with previous findings that ROS [40], sepsis [41], or endotoxemia [42] negatively affects ATP synthase activity, the late inhibition and depletion of ATP synthase subunit β observed in the present study 36 h after the onset of CLP may also explain mitochondrial uncoupling of ATP production from oxygen consumption.…”
Section: Discussionsupporting
confidence: 92%
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“…However, contrary to what could be expected, this was not associated with an increase in oxidative phosphorylation efficiency ATP/O, which could have compensated for energy production (ATP generation) from mitochondrial respiratory chain oxidative loss. This result reinforces and extends previous data [25] on the failure of the mitochondrial oxidative phosphorylation system to adjust its intrinsic coupling efficiency in early septic shock. Furthermore, in line with previous findings that ROS [40], sepsis [41], or endotoxemia [42] negatively affects ATP synthase activity, the late inhibition and depletion of ATP synthase subunit β observed in the present study 36 h after the onset of CLP may also explain mitochondrial uncoupling of ATP production from oxygen consumption.…”
Section: Discussionsupporting
confidence: 92%
“…Liver mitochondria were prepared using a differential centrifugation protocol [25]. Briefly, liver was immediately dissected and cut up finely with sharp scissors and diluted 1:10 ( w / v ) in ice-cold isolation medium consisting of 250 mM sucrose, 20 mM Tris/HCl, and 1 mM EGTA, pH 7.3.…”
Section: Methodsmentioning
confidence: 99%
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