Ecdysone Induced Gene Expression Is Associated with Acetylation of Histone H3 Lysine 23 in Drosophila melanogaster

Abstract

Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limited or no correlation with ecdysone induced transcriptional activity. We found that dCBP, which is encoded by the nejire gene, acetylates H3 lysine 23 in vivo, and silencing of nejire leads to reduced expression of the Eip74EF and Eip75B genes. Our results suggest that acetylation of specific lysine residues of histones contribute specifically to the dynamic regulation of transcription. Furthermore, along with previous studies identify CBP dependent H3 lysine 23 acetylation as an evolutionarily conserved chromatin modification involved in steroid induced gene activation.

20‐Hydroxyecdysone receptor‐activated Bombyx mori CCAAT/enhancer‐binding protein gamma regulates the expression of BmCBP and subsequent histone H3 lysine 27 acetylation in Bo. mori

“…mori . Moreover, in D. melanogaster , H3K23 is one of the acetylation sites of DmCBP (Bodai et al ., ); however, BmCBP did not enhance H3K23 acetylation in this study (Fig. ), suggesting that CBP acetylation sites vary amongst insects.…”

“…germanica metamorphosis (Fernandez-Nicolas and Belles, 2016). Drosophila CBP is also involved in establishing the DNA replication checkpoint during DNA synthesis (Blander et al, 2002;Pichierri and Franchitto, 2004;Smolik and Jones, 2007), and has been found to acetylate histone H3 lysine 23 and regulate the transcription of ecdysteroid pathway genes (Bodai et al, 2012). In Camponotus floridanus (Florida carpenter ant), CBP regulates foraging and scouting behaviours by catalysing histone acetylation (Simola et al, 2016).…”

Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire

“…Also, mammalian Moz targets H3K9 in vivo and regulates the expression of Hox genes, but the global H3K9Ac levels are not significantly affected in the homozygous Moz mutant (Voss et al 2009), indicating that other HATs have overlapping substrate specificity with Moz. In flies, a previous study had reported that the H3K23Ac levels were reduced 35% in nejire (nej) mutant embryos, which lack functional CBP/p300 (Bodai et al 2012). However, knocking down nej by dsRNA in S2 cells severely reduced levels of H3K27Ac but had no obvious effect on global levels of H3K23Ac (Tie et al 2009).…”

“…Another study showed that the plant homeodomain (PHD)-bromodomain of TRIM24, a coactivator for estrogen receptor a in humans, binds to unmodified H3K4 and acetylated H3K23 within the same H3 tail (Tsai et al 2010). Also, the levels of H3K23Ac at two ecdysone-inducible genes, Eip74EF and Eip75B, have been shown to correlate with the transcriptional activity of these two genes at the pupal stage, suggesting the involvement of H3K23Ac in ecdysone-induced transcriptional activation (Bodai et al 2012). In this study, we further provided evidence for the activating role of the Enok-mediated H3K23Ac mark in transcriptional regulation.…”

Mechanisms of transcriptional regulation of ecdysone response

“…FBgn0041092 Bai et al, 2000;Xie et al, 2015;Zhang et al, 2015 Interacts Zraly et al, 2006;Mazina et al, 2018 SWI/SNF Activation and repression of ecdysone-dependent genes Neiire (CBP/p300) FBgn0261617 Kirilly et al, 2011;Bodai et al, 2012;Mazina et al, 2018 Interacts with EcR in ecdysone-dependent way, promotes Histone H3 K27 acetylation at ecdysone-dependent genes, is important for ecdysone-dependent transcription activation…”