Echinococcus granulosus: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with rEg.myophilin

Sun, Junfeng; Wang, Yana; Li, Zongji; Ma, Rui; Ji, Haiqing; Xiong, Ying; Wang, Yin; Li, Zhaoyu; Zhao, Wei

doi:10.1007/s11259-010-9456-4

Cited by 9 publications

(10 citation statements)

References 19 publications

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“…Furthermore, the immune response and the induced immunoprotection of recombinant Eg.myophilin (rEg.myophilin) were investigated using an experimental model of hydatidosis in mice challenged with E. granulosus protoscoleces (14). It is also known that cytokines and serum antibodies serve an important role in CHD.…”

Section: Cystic Hydatid Disease (Chd) Is a Results Of Infection Withmentioning

confidence: 99%

“…In addition, rEg.myophilin has been reported to show an effectively protective immunity in mice (14). However, these experiments present two deficiencies: E. granulosus protoscoleces were intraperitoneally inoculated (secondary infection) due to the experimental condition at the time of the experiments, and thus the natural infection mode was peroral with E. granulosus eggs.…”

Section: Discussionmentioning

confidence: 99%

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Immunoprotection of recombinant Eg.myophilin against Echinococcus granulosus infection in sheep

Zhu

Gao

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. The aims of the present study were to investigate the immunoprotection of recombinant Echinococcus granulosus myophilin (rEg.myophilin) against the establishment of a challenge oral infection with E. granulosus eggs, as well as to determine the mechanisms underlying this protection. Sheep were subcutaneously immunized two times with rEg.myophilin, followed by the challenge with E. granulosus eggs orally. The animals were sacrificed 44 weeks after infection and the immunoglobulin (Ig) and cytokine levels were analyzed using ELISA. The results identified significant changes in several indexes of animal immune response subsequent to immunization with rEg.myophilin. These changes included reduced number of formed cysts, as well as elevated levels of IgG, IgA and cytokines. The present data suggest that immunization with rEg.myophilin in sheep can successfully reduce the formation of cysts caused by challenge E. granulosus infection and stimulate immune response, suggesting that rEg.myophilin a has potential value as a candidate vaccine against E. granulosus.

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Section: Cystic Hydatid Disease (Chd) Is a Results Of Infection Withmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunoprotection of recombinant Eg.myophilin against Echinococcus granulosus infection in sheep

Zhu

Gao

et al. 2016

Experimental and Therapeutic Medicine

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“…Previous studies have reported that the 14-3-3 protein (15), myophilin (16), ferritin (17) and P29 (18) that are secreted by E. granulosus exhibit marked immunogenic properties. The aim of the present study was to determine whether immunization with rEgGST was able to induce effective protective immunity when compared with a control group.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of protective immunity by vaccination with recombinant Echinococcus granulosus glutathione S-transferase (Chinese strain) in mice

Zhu

Wang

et al. 2015

Experimental and Therapeutic Medicine

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The aim of this study was to investigate the immunoprotective effects of recombinant glutathione S-transferase (rEgGST) against the development of protoscolices (PSCs), and to determine the mechanisms underlying this protection. ICR mice were subcutaneously immunized three times with rEgGST at weeks 0, 2 and 4, followed by the intraperitoneal administration of PSCs at week 10. Six mice in each group were sacrificed at 0, 2, 4, 6, 10, 18 and 30 weeks following the initial vaccination in order to observe the macroscopic and microscopic effects of parasite development. Various analyses were subsequently conducted, including determination of the levels of immunoglobulins (Igs) and cytokines. Significant differences were observed a number of indices of immune response following immunization with rEgGST. These included reduced cyst formation and elevated levels of IgG1, IgG2a, IgG3, IL-2, IL-4, IL-10 and IFN-γ, which indicated an increased percentage of immune helper cells. The results of the present study suggest that immunization with rEgGST in mice is able to successfully reduce the PSC-induced formation of cysts and to stimulate an immune response, suggesting that rEgGST possesses potential value as a candidate vaccine for PSC infection.

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“…Previous studies demonstrated that the native antigen model is not suitable for inducing protective immunity due to an increase in IL-10 levels that results in a Th2 response to different antigens such as EgA31 and EgTrp (Fraize et al 2005). Increased levels of IFN-γ and IL-4 have been observed in mice immunised with recombinant myophilin (Sun et al 2011). However, these proteins have the ability to produce a mixed Th1-Th2 response and the presence of a Th2 response may render the host capable of tolerating the presence of the parasite.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, Eg95 induced a significant immune response in sheep (Woollard et al 2000). Other reports discussed the roles of P14-3-3 (Siles-Lucas et al 2008), recombinant myophilin (Sun et al 2011) and phosphoglucose isomerase as protective antigens. However, none of these proteins has been found to induce relevant protectivity against E. granulosus in its definitive host.…”

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confidence: 99%

Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins

Esmaelizad

Ahmadian

Aghaiypour

et al. 2013

Mem. Inst. Oswaldo Cruz

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In this study, we designed an experiment to predict a potential immunodominant T-cell epitope and evaluate the protectivity of this antigen in immunised mice. The T-cell epitopes of the candidate proteins (EgGST, EgA31, Eg95, EgTrp and P14-3-3) were detected using available web-based databases. The synthesised DNA was subcloned into the pET41a+ vector and expressed in Escherichia coli as a fusion to glutathione-S-transferase protein (GST). The resulting chimeric protein was then purified by affinity chromatography. Twenty female C57BL/6 mice were immunised with the antigen emulsified in Freund's adjuvant. Mouse splenocytes were then cultured in Dulbecco's Modified Eagle's Medium in the presence of the antigen. The production of interferon-γ was significantly higher in the immunised mice than in the control mice (> 1,300 pg/mL), but interleukin (IL)-10 and IL-4 production was not statistically different between the two groups. In a challenge study in which mice were infected with 500 live protoscolices, a high protectivity level (99.6%) was demonstrated in immunised BALB/C mice compared to the findings in the control groups [GST and adjuvant (Adj) ]. These results demonstrate the successful application of the predicted T-cell epitope in designing a vaccine against Echinococcus granulosus in a mouse model.

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Echinococcus granulosus: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with rEg.myophilin

Cited by 9 publications

References 19 publications

Immunoprotection of recombinant Eg.myophilin against Echinococcus granulosus infection in sheep

Immunoprotection of recombinant Eg.myophilin against Echinococcus granulosus infection in sheep

Mechanism of protective immunity by vaccination with recombinant Echinococcus granulosus glutathione S-transferase (Chinese strain) in mice

Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins

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