Echinostoma revolutum: Development of a high performance DNA-specific primer to demonstrate the epidemiological situations of their intermediate hosts

Anucherngchai, Sothorn; Chontananarth, Thapana

doi:10.1016/j.actatropica.2018.09.014

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…E. revolutum has been shown to endemically infect humans in Southeast Asia [7,42]. In Thailand, it has been found in Northern, Northeastern, and Central parts, i.e., the metacercarial stage from freshwater snails and adult stage from poultry [9,10,17,20,21].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, freshwater snails can be the first and second intermediate host of echinostomes [13][14][15][16]. Many species of freshwater snails have been found to be infected with the metacercariae of echinostomes in Thailand, including Filopaludina spp., Anentome helena, Bithynia spp., and Eyriesia eyriesi [17][18][19][20][21]. The echinostome infections of snails have been recorded in some provinces, including Chiang Mai, Chiang Rai (Mae Lao district), and Lamphun [17][18][19]22].…”

Section: Introductionmentioning

confidence: 99%

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Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand

Butboonchoo

Wongsawad

et al. 2020

Korean J Parasitol

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Echinostome metacercariae were investigated in freshwater snails from 26 districts in 7 provinces of upper northern Thailand. The species identification was carried out based on the morphologies of the metacercariae and adult flukes harvested from experimental hamsters, and on nucleotide sequences of internal transcribed spacer 2 (ITS2) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes. Twenty-four out of 26 districts were found to be infected with echinostome metacercariae in freshwater snails with the prevalence of 40.4%. The metacercariae were found in all 6 species of snails, including Filopaludina martensi martensi (21.9%), Filopaludina doliaris (50.8%), F. sumatrensis polygramma (61.3%), Bithynia siamensis siamensis (14.5%), Bithynia pulchella (38.0%), and Anenthome helena (4.9%). The echinostome metacercariae found in these snails were identified as Echinostoma revolutum (37-collar-spined) and Echinostoma macrorchis (45-collar-spined) morphologically and molecularly. The 2-week-old adult flukes of E. revolutum revealed unique features of the cirrus sac extending to middle of the ventral sucker and smooth testes. E. macrorchis adults revealed the cirrus sac close to the right lateral margin of the ventral sucker and 2 large and elliptical testes with slight indentations and pointed posterior end of the posterior testis. The ITS2 and nad1 sequences confirmed the species identification of E. revolutum, and the sequences of E. macrorchis have been deposited for the first time in Gen-Bank. The presence of the life cycle of E. macrorchis is a new record in Thailand and the snail F. doliaris as their second intermediate host seems to be new among the literature.

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