ECM proteins involved in cell migration and vessel formation compromise bovine cloned placentation

Barreto, Rodrigo da Silva Nunes; Matias, Gustavo de Sá Schiavo; Nishiyama, Milton Yutaka; Carreira, Ana Cláudia Oliveira; Miglino, Maurizio

doi:10.1016/j.theriogenology.2022.04.003

Cited by 5 publications

(2 citation statements)

References 69 publications

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“…Among these KEGG terms, ‘Cytokine-cytokine receptor interaction’ is key in luminal epithelium proliferation, trophoblast development, and uNK maturation during pregnancy 39 . ‘ECM-receptor interaction’ is involved in cell migration and vessel formation in placentation 40 , especially in trophoblast giant cell migration 41 . Trophoblast giant cells lie at the edge of junctional zone, next to the maternal decidua in mouse.…”

Section: Resultsmentioning

confidence: 99%

Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature

Zhang,

He,

et al. 2024

Commun Biol

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The placenta is a unique organ for ensuring normal embryonic growth in the uterine. Here, we found that maternal RNA transcription in Dlk1-Dio3 imprinted domain is essential for placentation. PolyA signals were inserted into Gtl2 to establish a mouse model to prevent the expression of maternal RNAs in the domain. The maternal allele knock-in (MKI) and homozygous (HOMO) placentas showed an expanded junctional zone, reduced labyrinth and poor vasculature impacting both fetal and maternal blood spaces. The MKI and HOMO models displayed dysregulated gene expression in the Dlk1-Dio3 domain. In situ hybridization detected Dlk1, Gtl2, Rtl1, miR-127 and Rian dysregulated in the labyrinth vasculature. MKI and HOMO induced Dlk1 to lose imprinting, and DNA methylation changes of IG-DMR and Gtl2-DMR, leading to abnormal gene expression, while the above changes didn’t occur in paternal allele knock-in placentas. These findings demonstrate that maternal RNAs in the Dlk1-Dio3 domain are involved in placental vasculature, regulating gene expression, imprinting status and DNA methylation.

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Section: Resultsmentioning

confidence: 99%

Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature

Zhang,

He,

et al. 2024

Commun Biol

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“…ECM receptors are membrane proteins on the cell surface, and they can interact with ECM structural proteins. Previous research has shown that the ECM–receptor interaction plays an important regulatory role in follicle maturation, ovulation, and regression [ 55 ]. This process involves multiple signaling pathways and molecular mechanisms, which are of great significance for the normal function of the reproductive system.…”

Section: Discussionmentioning

confidence: 99%

Integrated miRNA and mRNA Sequencing Reveals the Sterility Mechanism in Hybrid Yellow Catfish Resulting from Pelteobagrus fulvidraco (♀) × Pelteobagrus vachelli (♂)

Li,

Yang,

et al. 2024

Animals

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The hybrid yellow catfish exhibits advantages over pure yellow catfish in terms of fast growth, fast development, a high feeding rate, and strong immunity; additionally, it is almost sterile, thus ensuring the conservation of the genetic stock of fish populations. To investigate the sterility mechanism in hybrid yellow catfish (P. fulvidraco (♀) × P. vachelli (♂)), the mRNA and miRNA of the gonads of P. fulvidraco, P. vachelli, and a hybrid yellow catfish were analyzed to characterize the differentially expressed genes; this was carried out to help establish gene expression datasets to assist in the further determination of the mechanisms of genetic sterility in hybrid yellow catfish. In total, 1709 DEGs were identified between the hybrid and two pure yellow catfishes. A KEGG pathway analysis indicated that several genes related to reproductive functions were upregulated, including those involved in the cell cycle, progesterone-mediated oocyte maturation, and oocyte meiosis, and genes associated with ECM–receptor interaction were downregulated. The spermatogenesis-related GO genes CFAP70, RSPH6A, and TSGA10 were identified as being downregulated DEGs in the hybrid yellow catfish. Sixty-three DEmiRNAs were identified between the hybrid and the two pure yellow catfish species. The upregulated DEmiRNAs ipu-miR-194a and ipu-miR-499 were found to target the spermatogenesis-related genes CFAP70 and RSPH6A, respectively, playing a negative regulatory role, which may underscore the miRNA–mRNA regulatory mechanism of sterility in hybrid yellow catfish. The differential expression of ipu-miR-196d, ipu-miR-125b, and ipu-miR-150 and their target genes spidr, cep85, and kcnn4, implicated in reproductive processes, was verified via qRT-PCR, consistent with the transcriptome sequencing expression trends. This study provides deep insights into the mechanism of hybrid sterility in vertebrate groups, thereby contributing to achieving a better understanding and management of fish conservation related to hybrid sterility.

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Late-gestation heat stress alters placental structure and function in multiparous dairy cows

Casarotto,

Jones,

Chavatte-Palmer

et al. 2025

Journal of Dairy Science

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ECM proteins involved in cell migration and vessel formation compromise bovine cloned placentation

Cited by 5 publications

References 69 publications

Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature

Maternal RNA transcription in Dlk1-Dio3 domain is critical for proper development of the mouse placental vasculature

Integrated miRNA and mRNA Sequencing Reveals the Sterility Mechanism in Hybrid Yellow Catfish Resulting from Pelteobagrus fulvidraco (♀) × Pelteobagrus vachelli (♂)

Late-gestation heat stress alters placental structure and function in multiparous dairy cows

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