Eco-friendly textile desizing with indigenously produced amylase from Bacillus cereus AS2

Abstract

Starch is added to the fabric surface to secure weaving process. During finishing these sized particles are removed from the fabric and prepared it for printing and dyeing. Chemicals de-sizing agents damage fabric surfaces and reduce the quality of the product. An alternative to these conventional desizing agents is the use of biological molecules i.e. enzymes. The current study compares traditional de-sizing to bio-based de-sizing methods, as well as the optimization of fabric desizing settings using crude amylase. Amylase-producing Bacillus cereus AS2 was isolated from indigenous soil samples. The maximal fermentative de-sizing capability was discovered at 72 h, with no fabric surface degradation. Chemical desizing showed that the fabric lost all sizing agents to TEGEWA scale 9 within 1 h in presence of 5N HCl. Optimal studies for desizing showed that 1000 IU/ml of amylase resulted in maximum de-sizing within 15 h at 60 °C and 0.5% Triton-X. Water absorbance and weight loss, both parameters were used to check the desizing efficacy and it was found that de-sizing to same scale was occurred in the case of enzyme as well as commercially desized fabric. Enzyme desized cloth was found to be free of any starch particles in SEM micrographs, identical to industrially de-sized fabric, ensuring bioprocess efficacy.

A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing

“…The complex enzymes were prepared by amylase (5 g/L), AlLPMO13 (0 mg/L, 3 mg/L, 5 mg/L and 7 mg/L), H 2 O 2 (0 g/L and 3 g/L) and penetrant JFC (fatty alcohol-polyoxyethylene ether) (5 g/L). The final dry cotton fabrics were used to test the desizing efficacy using the TEGEWA rating system [ 11 ] and measure their capillary effects [ 22 ]. The capillary effects of the treated cotton fabric both on the radial and weft directions were determined by the method reported in our previous reports [ 22 ].…”

“…Starch, as the second major reservoir of carbohydrates, is not only the major constituent of the human diet but also importance in various industries [ 11 ]. Starch and its derivatives account for over 75% of all sizing agents used in the textile industry around the world [ 11 ]. Desizing, souring and bleaching are preparatory stages for wet-processing treatments on cotton fabrics [ 11 , 12 ].…”

“…Starch and its derivatives account for over 75% of all sizing agents used in the textile industry around the world [ 11 ]. Desizing, souring and bleaching are preparatory stages for wet-processing treatments on cotton fabrics [ 11 , 12 ]. The removal of adhesive compounds (starch and its derivatives) from fabric threads is known as desizing.…”

“…The removal of adhesive compounds (starch and its derivatives) from fabric threads is known as desizing. The adhesive compounds must be applied during the weaving process to prevent yarn from damage and breakage [ 11 , 13 ]. Amylase has been used in the textile industry to remove starch residues from fabric [ 11 , 13 ].…”

“…The adhesive compounds must be applied during the weaving process to prevent yarn from damage and breakage [ 11 , 13 ]. Amylase has been used in the textile industry to remove starch residues from fabric [ 11 , 13 ]. To improve the operational efficient and simultaneously reduce its cost, amylases have to be added along with other auxiliary enzymes during the treatment steps [ 13 ].…”