Ecofriendly high‐performance thin‐layer chromatography‐densitometric method for determination of a new triple pharmaceutical combination targeting Helicobacter pylori eradication along with greenness profile assessment

Abstract

Herein, an ecofriendly, simple, accurate, and cost‐effective high‐performance thin‐layer chromatography‐densitometic method was developed and optimized for separation and estimation of the novel triple therapy combination of Tinidazole, Clarithromycin, and Lansoprazole for treatment of Helicobacter pylori infection. Successful chromatographic separation was established using high‐performance thin‐layer chromatography silica gel 60 F254 plates as stationary phase and a developing system consisted of ethanol‐acetone‐ammonia (10.0:4.0:0.1, by volume). Scanning of the bands was accomplished at 210 nm. The presented method has been fully validated and found to be suitable for application in routine quality control of the studied drugs in their dosage form with average percent recoveries of 99.97 ± 0.62, 100.89 ± 0.51, and 99.78 ± 0.56% for Clarithromycin, Tinidazole and Lansoprazole, respectively. The developed method was assessed with four greenness evaluation tools; National Environmental Method Index, Analytical Eco‐scale, Green Analytical Procedure Index, and Analytical GREEnness Metric tool. The suggested method was demonstrated to be more environmental friendly than the reported high‐performance liquid chromatography one in terms of the use of hazardous chemicals and solvents, energy consumption, and waste generation.

Mini Review on Different Analytical Approaches Applied on some Selected GIT acting Combination

“…At 210 nm, the bands' scanning was completed. The proposed approach has been extensively verified and is appropriate to use in routine quality control of the tested pharmaceuticals in their dosage form, with average percent recoveries of 99.97± 0.62, 100.89± 0.51, and 99.78± 0.56% for Clarithromycin, Tinidazole, and Lansoprazole, correspondingly (38).…”

Ecofriendly chromatographic estimation of spasmolytic pharmaceutical mixture along with official toxic impurity, 3,5‐dichloroaniline: Complete green profile appraisal

“…Green chemistry is considered a great challenging approach for introducing reliable, accurate, and effective methods without producing harm or hazard to humans and the environment via using green solvents, minimizing waste production, sample preparation, and energy of the instrument used [1]. Worldwide attention become focused on developing methods which were following the green analytical procedures [2][3][4][5]. Phloroglucinol (PHG), (1,3,5-trihydroxybenzene) Figure 1A, is antispasmodic drug, which has another uses in pharmaceuticals' synthesis, explosives and in printing as coupling agent.…”