Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Morton, Ana; Pino, Fernando García del

doi:10.1016/j.jip.2009.08.002

Cited by 41 publications

(38 citation statements)

References 53 publications

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“…SL0708 JIs is affected at temperatures below 20 °C while inexistent at 5° and 10 °C. The results are similar to those obtained by Chen et al (2003), Morton and Garcia-del-Pino (2009) and Saunders and Webster (1999), who report that at temperatures below 15 °C infection is not good, while at temperatures between 15 and 35 °C infectivity is optimal. In the case of Heterorhabditis sp.…”

Section: Discussionsupporting

confidence: 90%

“…It is recognised that temperature is an important factor in the life cycle of entomopathogenic nematodes (Griffin, 1993). Varying temperatures have effects on the viability of the IJs and their ability to reproduce, these effects are observed mainly in extreme temperatures (0 and 40 °C), which are lethal (Rohde et al, 2010;Susurluk, 2008;Morton and Garcia-del-Pino, 2009). As observed in Heterorhabditis sp.…”

Section: Discussionmentioning

confidence: 99%

“…SL0708, exposure to temperatures between 20 and 30 °C does not affect their viability over time, which is within the range established for other heterorhabditidae and some steinernematidae. An example of this is H. bacteriophora, individuals which can be recovered at temperatures up to 37 °C while other species such as Steinernema carpocapsae and Steinernema feltiae can only be recovered at temperatures up to 35 °C (Morton and Garcia-del-Pino, 2009). The infectivity of Heterorhabditis sp.…”

Section: Discussionmentioning

confidence: 99%

“…Most species of Heterorhabditis and Steinernema have been isolated from soil samples using Galleria mellonella larvae (L), (Lepidoptera: Pyralidae), in general, little information is provided on their biology and ecology and they are not complemented in subsequent studies, this may hinder their use as biological control agents in integrated pest management systems despite being obligated pathogens of a wide range of hosts (Hazir et al, 2004, Morton andGarcia-del -Pino, 2009), having a mutualistic relationship with bacteria of the Photorhabdus and Xenorhabdus genera (Adams et al, 2006), high virulence and specific ecological characteristics (Lewis et al, 2006;Rohde et al, 2010;Strauch et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Mejia-Torres

Sáenz

2013

Braz. J. Biol.

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The entomopathogenic nematode Heterorhabditis sp. SL0708 (Rhabditida: Heterorhabditidae) isolated from soil in Alcalá, Valle del Cauca (Colombia) was characterised ecologically using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts. The effect of temperature on the viability, infectivity and reproduction, and of moisture on infectivity and storage in liquid were evaluated in infective juveniles (IJs). Significant differences were found in the viability, infectivity and reproduction of the IJs at different temperatures. No nematodes were recovered at 5 °C and 10 °C, and at 35 °C no infectivity was observed. Average daily nematode recovery was best at 25 °C, and survival of the IJs was low in substrates presenting 13% moisture. The optimal storage temperature for Heterorhabditis sp. SL0708 was between 20 °C and 30 °C, keeping its infectivity for up to 8 weeks.Keywords: Rhabditida, infective juveniles, Heterorhabditidae, temperature, moisture, storage. Caracterização ecológica do nematoide entomopatogênico colombiano Heterorhabditis sp. SL0708Resumo O nematoide entomopatogênico colombiano Heterorhabditis sp. SL0708, isolado do solo de Alcalá, Valle del Cauca (Colombia), foi caracterizado ecologicamente utilizando-se como hospedeiro lagartas de Galleria mellonella (L) (Pyralidae:Galleriinae). O efeito da temperatura na viabilidade, na infecção e na reprodução, e da umidade na infecção e do armazenamento em líquido foi avaliado em juvenis infectantes (JIs). Diferenças significativas na viabilidade, na infecção e na reprodução dos JIs foram observadas nas diferentes temperaturas. Não foram recuperados nematoides a 5 °C e 10 °C, e não se observou infecção a 35 °C. A maior média diária de recuperação de nematoides foi a 25 °C. Por outro lado, a sobrevivência dos JIs foi baixa nos substratos com unidades de 13% e a melhor temperatura de armazenamento para SL0708 foi entre 20 °C e 30 °C, mantendo a infecção até oito semanas.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Mejia-Torres

Sáenz

2013

Braz. J. Biol.

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“…Foraging strategy was considered a species characteristic (Campbell & Gaugler, 1997), but nowadays many authors have proved that nematode's behaviour and virulence go down to the strain level (Wilson et al, 2012). Morton & García-del-Pino (2009) proved that often intra-specific differences are as important as inter-specific. When testing the tolerance and foraging behavior of different strains of S. feltiae towards different abiotic factors, these authors obtained different strain behaviors and virulence within the same species.…”

Section: Discussionmentioning

confidence: 99%

Field efficacy against the hazelnut weevil, Curculio nucum and short-term persistence of entomopathogenic nematodes

Batalla-Carrera¹,

Morton²,

Pino³

2013

Span. j. agric. res.

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The hazelnut weevil, Curculio nucum L. (Coleoptera: Curculionidae) is a pest affecting hazelnut orchards in Europe, with an important economical repercussion. Its potential control, short-term f ield persistence and the vertical distribution of native entomopathogenic nematode strains were tested in Muntanyes de Prades, Tarragona (NE Iberian Peninsula) over two consecutive years. Steinernema feltiae strain D114, Steinernema sp. strain D122 and Heterorhabditis bacteriophora strain DG46 were used in summer and spring applications at a dosage of 5 · 10 5 IJs m -2 . The three nematode species reduced the hazelnut weevil population, ranging from 32% to 88% efficacy, without significant differences in efficacy or between the two applications. Persistence evaluation was carried out during 9 weeks for S. feltiae (D114), Steinernema sp. (D122) and H. bacteriophora (DG46) and showed all species capable of lasting for this period. Nematodes and larval vertical distribution was assessed. Most of the hazelnut weevil stayed within the first 25 cm although some were found as deep as 40 cm. Entomopathogenic nematodes were found along all 40 cm depth. This study proves the suitability of entomopathogenic nematodes to control the hazelnut weevil.Additional key words: hazelnut orchard; biological control; Steinernema; Heterorhabditis; vertical distribution; spring application. * Corresponding author: Fernando.Garcia@uab.cat Received: 18-03-13. Accepted: 13-11-13.Abbreviations used: EPNs (entomopathogenic nematodes); GLZ (generalized linear model); HW (hazelnut weevil); IJ (infective juvenile).

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Commercialization of Biopesticides Based on Entomopathogenic Nematodes

Saleh

Metwally

Abonaem

2019

Cottage Industry of Biocontrol Agents and Their Applications

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Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of Mediterranean areas

Cited by 41 publications

References 53 publications

Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Field efficacy against the hazelnut weevil, Curculio nucum and short-term persistence of entomopathogenic nematodes

Commercialization of Biopesticides Based on Entomopathogenic Nematodes

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