Ecological separation and genetic isolation of Neisseria gonorrhoeae and Neisseria meningitidis

Vázquez, Julio; Fuente, Laura de la; Berrón, S.; O’Rourke, Maria; Smith, Noel H.; Zhou, Jiaji; Spratt, Brian G.

doi:10.1016/0960-9822(93)90001-5

Cited by 59 publications

(48 citation statements)

References 22 publications

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“…However, it would be possible to address some of the questions by analyzing hTf binding properties of hybrid Thp2 hTf binding domains (B16B6/M982). This approach seems to be interesting, because it is believed that genetic modifications frequently occur by DNA uptake and subsequent genetic recombinations in Neisseria species (see reference 24). Also, such stable structures should involve a high number of specific interactions; however, their nature could be significantly different in such divergent Thp2s.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin

et al. 1994

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The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highly structured and extremely stable Tbp2 domain of about 270 to 290 amino acids which is involved in the binding to transferrin and whose position is well conserved has been characterized. The conservation of such a remarkable structure in a very divergent protein domain (there is only 43% amino acid identity within this region) suggests that is plays an essential biological role and raises a number of questions regarding tbp2 evolution.

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Section: Resultsmentioning

confidence: 99%

Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin

et al. 1994

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“…A key finding of this study was the discovery of the gonococcal AniA/NorB denitrification system in the US meningococcal urethritis clade that enabled robust anaerobic growth. Considering the uniformity of housekeeping genes, N. gonorrheae may have evolved from a subset of meningococci that could colonize the genital tract (39). The further speciation of the gonococcus and meningococcus is suggested to be a consequence of the adaptation to different environments (46).…”

Section: Discussionmentioning

confidence: 99%

Emergence of a new Neisseria meningitidis clonal complex 11 lineage 11.2 clade as an effective urogenital pathogen

Tzeng

Bazan

Turner

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

124

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Neisseria meningitidis (Nm) clonal complex 11 (cc11) lineage is a hypervirulent pathogen responsible for outbreaks of invasive meningococcal disease, including among men who have sex with men, and is increasingly associated with urogenital infections. Recently, clusters of Nm urethritis have emerged primarily among heterosexual males in the United States. We determined that nonencapsulated meningococcal isolates from an ongoing Nm urethritis outbreak among epidemiologically unrelated men in Columbus, Ohio, are linked to increased Nm urethritis cases in multiple US cities, including Atlanta and Indianapolis, and that they form a unique clade (the US Nm urethritis clade, US_NmUC). The isolates belonged to the cc11 lineage 11.2/ET-15 with fine type of PorA P1.5–1, 10–8; FetA F3-6; PorB 2–2 and express a unique FHbp allele. A common molecular fingerprint of US_NmUC isolates was an IS1301 element in the intergenic region separating the capsule ctr-css operons and adjacent deletion of cssA/B/C and a part of csc, encoding the serogroup C capsule polymerase. This resulted in the loss of encapsulation and intrinsic lipooligosaccharide sialylation that may promote adherence to mucosal surfaces. Furthermore, we detected an IS1301-mediated inversion of an ∼20-kb sequence near the cps locus. Surprisingly, these isolates had acquired by gene conversion the complete gonococcal denitrification norB-aniA gene cassette, and strains grow well anaerobically. The cc11 US_NmUC isolates causing urethritis clusters in the United States may have adapted to a urogenital environment by loss of capsule and gene conversion of the Neisseria gonorrheae norB-aniA cassette promoting anaerobic growth.

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“…In fact, it has been suggested that the N. gonorrhoeae species may have arisen from a clone of N. meningitidis that was able to colonize the genital tract. 58,59 Second, the Neisseria species are unusual in that they are fully competent for DNA uptake throughout their entire life cycle. This enables frequent interspecific horizontal genetic exchange within the Neisseria genus.…”

Section: Sources Of Cross-reactionmentioning

confidence: 99%

Nucleic Acid Amplification Testing for Neisseria gonorrhoeae

Whiley

Tapsall

Sloots

2006

The Journal of Molecular Diagnostics

178

163

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Nucleic acid amplification tests (NAATs) for the detection of Neisseria gonorrhoeae became available in the early 1990s. Although offering several advantages over traditional detection methods, N. gonorrhoeae NAATs do have some limitations. These include cost, risk of carryover contamination, inhibition, and inability to provide antibiotic resistance data. In addition, there are sequence-related limitations that are unique to N. gonorrhoeae NAATs. In particular, falsepositive results are a major consideration. These primarily stem from the frequent horizontal genetic exchange occurring within the Neisseria genus, leading to commensal Neisseria species acquiring N. gonorrhoeae genes. Furthermore, some N. gonorrhoeae subtypes may lack specific sequences targeted by a particular NAAT. Therefore, NAAT false-negative results because of sequence variation may occur in some gonococcal populations. Overall, the N. gonorrhoeae species continues to present a considerable challenge for molecular diagnostics. The need to evaluate N. gonorrhoeae NAATs before their use in any new patient population and to educate physicians on the limitations of these tests is emphasized in this review.

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Ecological separation and genetic isolation of Neisseria gonorrhoeae and Neisseria meningitidis

Cited by 59 publications

References 22 publications

Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin

Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin

Emergence of a new Neisseria meningitidis clonal complex 11 lineage 11.2 clade as an effective urogenital pathogen

Nucleic Acid Amplification Testing for Neisseria gonorrhoeae

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