Ecotoxicological effects of the herbicide glyphosate in non-target aquatic species: Transcriptional responses in the mussel Mytilus galloprovincialis

Milan, Massimo; Rovere, Giulia Dalla; Smits, Morgan; Ferraresso, Serena; Pastore, Paolo; Marin, María Gabriella; Bogialli, Sara; Patarnello, Tomaso; Bargelloni, Luca; Matozzo, Valerio

doi:10.1016/j.envpol.2018.02.049

Cited by 56 publications

(24 citation statements)

References 82 publications

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“…Following the results of the late Andrés Carrasco and his research group (Paganelli et al, 2010;Carrasco, 2013), recent findings in environmental toxicology of glyphosate and its formulated products include its revealed toxicity on amphibian species (Mann et al, 2009;Relyea and Jones, 2009;Meza-Joya et al, 2013;Wagner et al, 2013;Henao Muñoz et al, 2015;Baier et al, 2016a,b), on mollusks (Conners and Black, 2004) and on earthworms (Zaller et al, 2014;Gaupp-Berghausen et al, 2015). It affected hemocyte parameters and acetylcholinesterase (AChE) activity, but not antioxidant enzyme activities in mussel Mytilus galloprovincialis (Matozzo et al, 2018) with disruption of key biological processes including energy metabolism, Ca 2+ homeostasis and endoplasmic reticulum stress response, as well as cell signaling identified by transcriptome analysis (Milan et al, 2018). Glyphosate exerted acute toxicity on the invasive snail Pomacea canaliculata with a 96-h LC 50 value of 175 mg/l, overly high for control purposes, but indicating oxidative stress, enhanced overall metabolic rate and altered catabolism from protein to carbohydrate/lipid mode (Xu et al, 2017).…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

106

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“…In particular, RNA-Seq is the favored sequencing technique to obtain knowledge on the functional response of organisms to environmental conditions. Indeed, in a multistress context, this technique enables us to depict the alteration of several genes and molecular pathways simultaneously in nonmodel organisms in response to stressful conditions [33][34][35] . ddRAD sequencing is one of the most powerful approaches to resolve fine-scale population structures compared with microsatellites, since ddRAD enables researchers to obtain thousands of markers 36,37 .…”

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confidence: 99%

Omics approaches for conservation biology research on the bivalve Chamelea gallina

Carducci

Biscotti

Trucchi

et al. 2020

Sci Rep

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The striped venus (Chamelea gallina) is an important economic resource in the Mediterranean Basin; this species has exhibited a strong quantitative decline in the Adriatic Sea. The aim of this work was to provide a comprehensive view of the biological status of C. gallina to elucidate the bioecological characteristics and genetic diversity of wild populations. To the best of our knowledge, this investigation is the first to perform a multidisciplinary study on C. gallina based on two omics approaches integrated with histological, ecotoxicological, and chemical analyses and with the assessment of environmental parameters. The results obtained through RNA sequencing indicated that the striped venus has a notable ability to adapt to different environmental conditions. Moreover, the stock reduction exhibited by this species in the last 2 decades seems not to have negatively affected its genetic diversity. Indeed, the high level of genetic diversity that emerged from our ddRAD dataset analyses is ascribable to the high larval dispersal rate, which might have played a “compensatory role” on local fluctuations, conferring to this species a good adaptive potential to face the environmental perturbations. These findings may facilitate the efforts of conservation biologists to adopt ad hoc management plans for this fishery resource.

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“… 43 with Wistar rat testis or Sertoli cells exposed to another glyphosate-based formulation (Roundup®), the authors reported a Ca 2+ imbalance and proposed that the formulation promotes an intracellular Ca 2+ overload and subsequent cell signaling misregulation, potentially causing cellular stress. More recently, these Ca 2+ imbalance effects were also discussed in a study addressing effects of glyphosate in marine invertebrate Mytilus galloprovincialis 44 . After 7 days of exposure, the response of the organisms to a stressful condition was evidenced by over-representation of transcripts involved in oxidation-reduction processes (GO: GO:0055114; Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

Simões

Novais

Natal-da-Luz

et al. 2018

Sci Rep

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The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87–1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction.

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Ecotoxicological effects of the herbicide glyphosate in non-target aquatic species: Transcriptional responses in the mussel Mytilus galloprovincialis

Cited by 56 publications

References 82 publications

Re-registration Challenges of Glyphosate in the European Union

Re-registration Challenges of Glyphosate in the European Union

Omics approaches for conservation biology research on the bivalve Chamelea gallina

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

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